ПРОГРАМНЕ ЗАБЕЗПЕЧЕННЯ ВІДНОВЛЕННЯ РОЗПОДІЛІВ ЗА МАЛИМИ ВИБІРКАМИ

The program «SmallSample» for distributions restoration using small samples is developed.The distributions nonparametric restoration methods for small samples are compared.Разработано программное обеспечение «SmallSample» восстановления распределений по малым выборкам. Проведено сравнение работы непараметрических методов восстановления распределений по малым выборкам.Розроблено програмне забезпечення «SmallSample» відновлення розподілів за малими вибірками. Проведено порівняння роботи непараметричних методів відновлення розподілів за малими вибірками

Phosphatidylserine Targets Single-Walled Carbon Nanotubes to Professional Phagocytes In Vitro and In Vivo

Broad applications of single-walled carbon nanotubes (SWCNT) dictate the necessity to better understand their health effects. Poor recognition of non-functionalized SWCNT by phagocytes is prohibitive towards controlling their biological action. We report that SWCNT coating with a phospholipid “eat-me” signal, phosphatidylserine (PS), makes them recognizable in vitro by different phagocytic cells - murine RAW264.7 macrophages, primary monocyte-derived human macrophages, dendritic cells, and rat brain microglia. Macrophage uptake of PS-coated nanotubes was suppressed by the PS-binding protein, Annexin V, and endocytosis inhibitors, and changed the pattern of pro- and anti-inflammatory cytokine secretion. Loading of PS-coated SWCNT with pro-apoptotic cargo (cytochrome c) allowed for the targeted killing of RAW264.7 macrophages. In vivo aspiration of PS-coated SWCNT stimulated their uptake by lung alveolar macrophages in mice. Thus, PS-coating can be utilized for targeted delivery of SWCNT with specified cargoes into professional phagocytes, hence for therapeutic regulation of specific populations of immune-competent cells