MORPHOGENESIS AND HlSTOMORPHOLOGY OF THE SCLERA IN LAYER AND BROILER CHICKEN*

ABSTRAC

Histogenesis of Peyer’s patches in Ovine foetus (Ovis aries)

Tissue pieces of jejunum and ileum from different prenatal age groups of sheep were collected from Corporation slaughter house, Perambur, Chennai. By three months of foetal age in sheep, the Peyer’s patches appeared as an aggregation of lymphocytes in the propria submucosa of the jejunum and ileum. The lymphocytic aggregation appeared only in the antimesenteric part of the jejunum and ileum. By four months of foetal age, the circumscribed nodular aggregations of lymphocytes were found enlarged giving a follicle-like appearance. The capsular connective tissue was predominated by reticular fibres and a few collagen fibres. The dome area of the follicle consisted of closely packed small-sized lymphocytes which appeared darker than the basal area. The smooth muscle fibres of muscularis mucosae were not continuous throughout and were absent in the follicle having domes. In five months-old foetuses, the jejunal and ileal Peyer’s patches were distinctly observed as follicles. The follicle showed a distinct outer cortex and an inner lighter medulla. Numerous small-sized lymphocytes were observed in the outer cortex and few lymphoblasts, medium sized lymphocytes and reticular cells were observed in the medulla. Keywords: Histogenesis; Peyer’s patches; Ovine foetus

POSTNATAL DIFFERENTIATION OF SPERMATOGENIC CELLS IN THE TESTIS OF RAM*

ABSTRAC

Age-related changes in cell yield and viability of feline Adipose Tissue-Derived Mesenchymal Stem (fAD-MSCs)

In the present study, omental adipose tissue was collected from, the animals that underwent ovariectomy and ovariohysterectomy, surgical procedures, at the age of seven months to 11/2 years of age groups. The sample was digested with 0.1% (W/V) collagenase type I and transferred to a beaker with a magnetic stirrer and kept in a stirrer with 600 rpm at 37 °C for 30 minutes. The viability of the cell was evaluated by the trypan blue exclusion method using a hemocytometer. Trypan blue had a high affinity to nuclear DNA, which traverse the member in a dead cell and dye it blue. In the present study, the cell yield of fAD-MSCs was 8.15 ± 0.68, 6.55 ± 0.26, 4.85 ± 0.42, 3.90 ± 0.34, and 3.51 ± 0.43 in different age groups viz., 7,8,9 month 1 and 1½ year respectively. In younger age groups, cell yield and viability percentage were more than in animals of higher age groups. In the younger age group, stem cells proliferation status is considered potent for therapeutic application