Evaluation of the Effect of Low-Frequency Electromagnetic Fields on in Vitro Growth and Maturation of Mouse Oocytes

Introduction: Access to modern methods for increasing the percentage of in vitro human and animal mature oocytes can be useful in the treatment of some forms of human infertility as well as proliferation of many domestic and wild animals which generation is endangered. Effect of low- frequency electromagnetic fields on in vitro growth and maturation of mouse oocytes is recently considered as a new approach. In this study we evaluated the effect of low- frequency electromagnetic field on in vitro growth and maturation of mouse oocyte. Methods: In this study electromagnetic fields with frequencies of 5, 50 and 100 Hz and 2mT intensity were used. For observation of the effect of electromagnetic field four groups were selected: Group 1 as control group, which included 35 prenatal follicles (immature oocytes). Groups 2, 3 and 4were exposed to 5, 50 and 100 Hz electromagnetic fields, respectively. Results: Prenatal follicles exposed to 5 and 50 Hz frequencies showed no significant changes in diameter and survival rates. In contrast at a frequency of 100 Hz in 72-hour culture period a significant increase in diameter(155μm), follicles livability power(59%), oocyte maturation(52%) and GVBD(39%) was shown in comparison to other experimental groups and control group(P <0.05). Conclusion: Low-frequency magnetic field effects gene expression and thus protein synthesis, cell division, proliferation and behavior. Although this effect can be temporary, it can increase the percentage of ovulation for in vitro environment along with other environmental factors

Laboratory study: evaluation and comparison of physicochemical properties, level of surface charge and cytotoxicity of lipid nanocomposites for carrying nucleic acid in bone cancer

Introdution: The aim of this study was to investigate the physico-chemical properties, surface charge and cytotoxicity of nanocomposite formulations for the design of lipid nano-systems with maximum loading for nucleic acids in bone marrow cancer. Methods For this study, six types of liposomes with different compositions were synthesized using DPPC, cholesterol, DSPE-mPEG (2000) and DOTAP. Then, nanosystems were evaluated for particle size, surface charge, cytotoxicity and loading rate of miRNA in two MG-63 and SaOs2 cell lines, which are human cell bone marrow cell lines. Results: All nano-systems were monodisperse. Among the systems, the F6 formula has the lowest toxicity and the highest load of miRNA-143, due to the presence of a suitable amount of DOTAP and PEG phospholipid in the liposome structure. Conclusion: Liposomal formulation with a suitable percentage of DOTAP cationic phospholipid can be used as a successful carrier for the transport of miRNA in the gene therapy process for the treatment of various cancers, especially metastatic types

Comparative Study of Antimicrobial Activities of TiO 2 and CdO Nanoparticles against the Pathogenic Strain of Escherichia coli

ABSTRACT Background: and Objectives: The aim of this study was to detect the antibacterial properties o