36 research outputs found
Assessing the Impact of Agricultural Research: Data Requirements and Quality of Current Statistics in Europe
Assessing the impact of agricultural research on sustainability targets often implies to face two main issues: the complexity of the causal path, and the lack of appropriate data. In this paper, we discuss which data would be necessary to measure short- and long-term impacts in Europe, and suggest a set of indicators to evaluate their quality, exploiting both available metadata (qualitative indicator) and the evidence stemming from the data themselves (quantitative indicator based on missing values, temporal contiguity and outliers). An application is shown for a subset of variables. According to our results, qualitative and quantitative indicators often provide conflicting information
Assessing the impact of agricultural research: Data requirements and quality of current statistics in Europe
Assessing the impact of agricultural research on sustainability targets often implies to face two main issues: the complexity of the causal path, and the lack of appropriate data. In this paper, we discuss which data would be necessary to measure short- and long-term impacts in Europe, and suggest a set of indicators to evaluate their quality, exploiting both available metadata (qualitative indicator) and the evidence stemming from the data themselves (quantitative indicator based on missing values, temporal contiguity and outliers). An application is shown for a subset of variables. According to our results, qualitative and quantitative indicators often provide conflicting information
The Rapid Identification of Anoplophora chinensis (Coleoptera: Cerambycidae) From Adult, Larval, and Frass Samples Using TaqMan Probe Assay
A molecular diagnostic method using TaqMan probe qPCR is presented for the identification of Anoplophora chinensis (Förster) (Coleoptera: Cerambycidae) from whole body insects (adults and larvae) and frass samples stored under different conditions. The results showed a perfect amplification of DNA from all samples; the repeatability and reproducibility of the protocol were very good, with standard deviations of inter-run and intrarun variability less than or equal to 0.5. The assay allowed to discern all A. chinensis samples from those of the other non-target wood-borer species, with 100% correspondence to the homologous sequences. No amplification or cross reactions were observed with A. glabripennis (Motschulsky) (Coleoptera: Cerambycidae), which is the most related species among those tested. The protocol was validated by an internal blind panel test which showed a good correspondence between the results obtained by different operators in the same lab. The analytical sensitivity for the lab frass with the Probe qPCR, namely the lowest amount of A. chinensis DNA that can be detected (LoD), was 0.64 pg/μl with a Cq of 34.87. The use of indirect evidence for the identification of a pest is an important feature of the method, which could be crucial to detect the presence of wood-boring insects.
This diagnostic tool can help prevent the introduction of A. chinensis into new environments or delimit existing outbreak areas thanks to indirect frass diagnosis
First record of Aleurocanthus camelliae Kanmiya & Kasai, 2011 (Hemiptera, Aleyrodidae) from Italy, on ornamental Camellia spp. plants
This paper provides a first report of Aleurocanthus camelliae, the Camellia spiny whitefly, from Italy. The pest was found on plants of Camellia spp. grown in the nursery. Brief morphological and biological information is provided on this whitefly, as well as some considerations on the phytosanitary measures to be adopted to reduce the potential risk of its spread on ornamental plants in Europe and the EPPO region
A duplex real-time PCR with probe for simultaneous detection of Geosmithia morbida and its vector Pityophthorus juglandis
The expression profiles of CD47 in the tumor microenvironment of salivary gland cancers: a next step in histology-driven immunotherapy
Background: Salivary gland carcinomas (SGC) are extremely rare malignancies with only limited treatment options for the metastatic phase of the disease. Treatment with anti-CD47 antibodies could represent a potent therapy for SGCs by promoting the phagocytic clearance of tumor cells through various mechanisms. However, the efficacy of anti-CD47 therapy is largely dependent on the expression of CD47 within the tumor microenvironment (TME). Materials and Methods: In 43 patients with SGC, we were the first to investigate the CD47 expression in both tumor cells and tumor-infiltrating immune cells (TIIC) in the center and periphery of primary tumors. We also correlated the data with the clinicopathological variables of the patients and offered novel insights into the potential effectiveness of anti-CD47 therapy in SGCs. Results: We observed that the CD47+ tumor cells are outnumbered by CD47+ TIICs in mucoepidermoid carcinoma. In the tumor center, the proportion of CD47+ tumor cells was comparable to the proportion of CD47+ TIICs in most histological subtypes. In low-grade tumors, significantly higher expression of CD47 was observed in TIICs in the periphery of the tumor as compared to the center of the tumor. Conclusion: The reason for a high expression of ‘don’t eat me’ signals in TIICs in the tumor periphery is unclear. However, we hypothesize that in the tumor periphery, upregulation of CD47 in TIICs could be a mechanism to protect newly recruited leukocytes from macrophage-mediated phagocytosis, while also allowing the removal of old or exhausted leukocytes in the tumor center