High-intensity exercise training induces morphological and biochemical changes in skeletal muscle

Skeletal muscle shows an elevated plasticity and can adapt its metabolic and contractile properties in response to a variety of stimuli such as physical exercise. This implies a series of biochemical and morphological changes in the recruited muscle, in order to produce the more appropriate functional response dependent on the specific stimulation. To determine the effective role of physical exercise in the muscle plasticity, in the present study we investigated the effect of two different exercise protocols on fiber composition and metabolism of two specific muscles of mice: the quadriceps -a fast-twitch muscle- and the gastrocnemius -a typical slow-twitch muscle. Mice were run daily on a motorized treadmill for 8 weeks, at a velocity corresponding to 60% (low-intensity exercise) or 90% (high-intensity exercise) of the maximal running velocity previously determined by an incremental exercise test. We found that at the end of training the body weight was significantly increased in highintensity exercise mice (18.2 ± 1.4 %) compared to low-intensity exercise (8.7 ± 0.6 %) and control (12.7 ± 0.5 %) groups, and it was lesser in low-intensity exercise mice compared to controls. In contrast, the food intake of both exercise training mice was greater compared to control group. Whereas low-intensity exercise mice, despite consumed significantly more food compared to control mice, increased the weight lesser, the weight increase of high-intensity exercise mice, that consumed significantly more food compared to other experimental groups, was significantly greater. These effects were accompanied by a progressive reduction in blood lactate levels at the end of training in both the exercised mice compared with controls; in particular, blood lactate levels after highintensity exercise were significantly lower than those measured in low-intensity exercise mice. Moreover, in the present study we demonstrated that high-intensity exercise training produced a significant increase in the expression of mitochondrial complex enzymes (significant for the enzymes corresponding to the Complex IV, II and I of mitochondrial chain) both in gastrocnemius and quadriceps muscle, compared with controls. These changes were associated with an increase in the amount of slow fibers in both these muscle of high-intensity exercise mice. No changing in the expression of mitochondrial enzymes and in the percentage of slow fibers were found in low-intensity exercise mice

Variable Admittance Control of a Hand Exoskeleton for Virtual Reality-Based Rehabilitation Tasks

Robot-based rehabilitation is consolidated as a viable and efficient practice to speed up and improve the recovery of lost functions. Several studies highlight that patients are encouraged to undergo their therapies and feel more involved in the process when collaborating with a user-friendly robotic environment. Object manipulation is a crucial element of hand rehabilitation treatments; however, as a standalone process may result in being repetitive and unstimulating in the long run. In this view, robotic devices, like hand exoskeletons, do arise as an excellent tool to boost both therapy's outcome and patient participation, especially when paired with the advantages offered by interacting with virtual reality (VR). Indeed, virtual environments can simulate real-life manipulation tasks and real-time assign a score to the patient's performance, thus providing challenging exercises while promoting training with a reward-based system. Besides, they can be easily reconfigured to match the patient's needs by manipulating exercise intensity, e.g., Assistance-As-Needed (AAN) and the required tasks. Modern VR can also render interaction forces when paired to wearable devices to give the user some sort of proprioceptive force or tactile feedback. Motivated by these considerations, a Hand Exoskeleton System (HES) has been designed to be interfaced with a variable admittance control to achieve VR-based rehabilitation tasks. The exoskeleton assists the patient's movements according to force feedback and following a reference value calculated inside the VR. Whenever the patient grasps a virtual object, the HES provides the user with a force feedback sensation. In this paper, the virtual environment, developed within the Webots framework and rendering a HES digital-twin mapping and mimicking the actual HES motion, will be described in detail. Furthermore, the admittance control strategy, which continuously varies the control parameters to best render the force sensation and adapt to the user's motion intentions, will be investigated. The proposed approach has been tested on a single subject in the framework of a pilot study

The challenge of developing Special Economic Zones in Africa: evidence and lessons learnt

Special economic zones (SEZs) are mushrooming across the developing world. Increasingly, policymakers resort to zones with the aim of turning around their countries’ economic fortunes. Zones are expected to deliver greater innovation, exports, knowledge and technological spillovers. Yet, little is known about the state of play of SEZs in Africa, where almost half of SEZ programmes are less than 10 years old. The recent proliferation of SEZs in the continent has rendered the need to ensure that SEZs deliver on their objectives more impelling, given the often non-negligible opportunity costs associated with SEZ development. This article addresses this knowledge gap and sheds light on African SEZ practices. The analysis of a novel dataset highlights that (i) African SEZs are on a steep upward trend and are changing in nature; (ii) the ability of African SEZs to attract industrial activity, proxied by firms, and generate employment remains limited; and (iii) African SEZ governance policies (over)rely on fiscal incentives and performance requirements. Case studies from Ethiopia, Morocco and South Africa suggest that those African SEZ programmes that have a well-targeted strategic focus, promote institutional collaboration and take a proactive approach to create linkages with the local economy are more likely to succeed

Molecular genetic features and risk assessment in a series of 30 patients who underwent an operation for gastrointestinal stromal tumours

Background: The objective of the study was to investigate the relationship between molecular genetic features and the standard criteria of risk assessment in patients affected by gastrointestinal stromal tumours (GISTs). Methods: A review was conducted of a series of 30 patients, with a mean age of 67 years, who underwent surgery for primary GISTs. R0 resection was accomplished in 27 patients. CD117, CD34 desmin, vimentin, S-100 and smooth muscle actin were immunohistochemically tested to achieve a diagnosis of GIST. The loss of wild-type KIT or platelet-derived growth factor receptor alpha (PDGFRα) genes was investigated by sequencing the tumour DNA. Results: Tumour genes mutations were reported in 23 patients (77%), and wild-type in seven. Mutations on the KIT gene occurred in 18 patients, and mutations on the PDGFRα gene in five. The average sizes of the GIST were 8.7 cm, 5.4 cm and 5.9 cm for KIT gene-mutated, PDGFRα gene-mutated and wild-type tumours, respectively. KIT gene mutations were detected in 50% of gastric and in 70% of extragastric GISTs. Moreover, 70% of tumours with a mitotic rate ≥ 5 x 50 highpower fields (HPFs) underwent KIT gene mutations. Conversely, PDGFRα mutations were observed only in gastric GISTs with a mitotic rate ≤ 5 x 50 HPFs. By stratifying GISTs according to classes of risk, KIT mutation was shown in most of the high-risk tumours. PDGFRα mutations occurred exclusively in lower classes of risk. Conclusion: Molecular analysis data might have a role as a prognostic variable in models of risk assessment for patients with GISTs

A weak lensing analysis of the PLCK G100.2-30.4 cluster

We present a mass estimate of the Planck-discovered cluster PLCK G100.2-30.4, derived from a weak lensing analysis of deep SUBARU griz images. We perform a careful selection of the background galaxies using the multi-band imaging data, and undertake the weak lensing analysis on the deep (1hr) r-band image. The shape measurement is based on the KSB algorithm; we adopt the PSFex software to model the Point Spread Function (PSF) across the field and correct for this in the shape measurement. The weak lensing analysis is validated through extensive image simulations. We compare the resulting weak lensing mass profile and total mass estimate to those obtained from our re-analysis of XMM-Newton observations, derived under the hypothesis of hydrostatic equilibrium. The total integrated mass profiles are in remarkably good agreement, agreeing within 1$\sigma$ across their common radial range. A mass $M_{500} \sim 7 \times 10^{14} M_\odot$ is derived for the cluster from our weak lensing analysis. Comparing this value to that obtained from our reanalysis of XMM-Newton data, we obtain a bias factor of (1-b) = 0.8 $\pm$ 0.1. This is compatible within 1$\sigma$ with the value of (1-b) obtained by Planck Collaboration XXIV from their calibration of the bias factor using newly-available weak lensing reconstructed masses.Comment: 11 pages, 12 figures, accepted for publication on Astronomy & Astrophysics; updates in affiliation

A newly identified galaxy group thanks to tidal streams of intragroup light

In the accretion-driven growth scenario, part of the intracluster light is formed in the group environment. We report the serendipitous discovery of a group of galaxies with signs of diffuse light in the foreground of the known galaxy cluster MACS J0329-0211 at z=0.45. Our investigation began with the detection of diffuse light streams around a pair of bright galaxies in the southeastern region of a Suprime-Cam image of the galaxy cluster MACS J0329-0211. Our analysis is based on the extended CLASH-VLT redshift catalog and on new spectroscopic data obtained ad hoc with the Italian Telescopio Nazionale Galileo. We use the density reconstruction method to analyze the redshift distribution of the galaxies in the region around the galaxy pair. We also use available photometric and X-ray data to better characterize the properties of the group. Thanks to the large amount of redshift data collected in this region, we have been able to discover the existence of a group of galaxies, here called GrG J0330-0218, which is associated with the pair of galaxies. These are the two brightest group galaxies (BGG1 and BGG2). We extracted 41 group members from the redshift catalog and estimate a mean redshift z=0.1537 and a line-of-sight velocity dispersion sigmav=370 km/s. In the phase-space diagram, the distribution of the galaxies of GrG J0330-0218 follows the characteristic trumpet-shaped pattern, which is related to the escape velocity of galaxy clusters, suggesting that the group is a virialized structure. Under this assumption, the mass of the group is M200 about 6E13 Msun. We also measured a mass-to-light ratio of 130 Msun/Lsun and a luminosity fraction of diffuse light of about 20% within 0.5 R200. We conjecture that galaxy pairs that are surrounded by diffuse light, probably due to tidal interactions, can serve as signposts for groups.Comment: Astronomy & Astrophysics accepted, 13 pages, 10 figure

The JAK2V617 mutation induces constitutive activation and agonist hypersensitivity in basophils of polycythemia vera.

BACKGROUND: The JAK2V617F mutation has been associated with constitutive and enhanced activation of neutrophils, while no information is available concerning other leukocyte subtypes. DESIGN AND METHODS: We evaluated correlations between JAK2V617F mutation and the count of circulating basophils, the number of activated CD63(+) basophils, their response in vitro to agonists as well as the effects of a JAK2 inhibitor. RESULTS: We found that basophil count was increased in patients with JAK2V617F -positive myeloproliferative neoplasms, particularly in those with polycythemia vera, and was correlated with the V617F burden. The burden of V617F allele was similar in neutrophils and basophils from patients with polycythemia vera, while total JAK2 mRNA content was remarkably greater in the basophils; however, the content of JAK2 protein in basophils was not increased. The number of CD63(+) basophils was higher in patients with polycythemia vera than in healthy subjects or patients with essential thrombocythemia or primary myelofibrosis and was correlated with the V617F burden. Ultrastructurally, basophils from patients with polycythemia vera contained an increased number of granules, most of which were empty suggesting cell degranulation in vivo. Ex vivo experiments revealed that basophils from patients with polycythemia vera were hypersensitive to the priming effect of interleukin-3 and to f-MLP-induced activation; pre-treatment with a JAK2 inhibitor reduced polycythemia vera basophil activation. Finally, we found that the number of circulating CD63(+) basophils was significantly greater in patients suffering from aquagenic pruritus, who also showed a higher V617F allele burden. CONCLUSIONS: These data indicate that the number of constitutively activated and hypersensitive circulating basophils is increased in polycythemia vera, underscoring a role of JAK2V617F in these cells’ abnormal function and, putatively, in the pathogenesis of pruritus

A radio bubble shredded by gas sloshing?

We report on the detection of diffuse radio emission with peculiar morphology in the central region of the galaxy cluster Abell 2657. The most striking feature identified in our 144 MHz LOFAR image is a bifurcated radio arc that extends for a projected size of 150-200 kpc. From the analysis of XMM-Newton data, we find clear evidence of gas sloshing in the cluster and a possible dip in X-ray surface brightness between the two radio arcs which deserves confirmation. Interestingly, the synchrotron emission of the bifurcated radio arc is stretched along the sloshing spiral. We compare our observational results with numerical simulations of non-thermal components interacting with gas motions. We suggest that the detected emission may trace a radio bubble shredded by gas sloshing, where relativistic electrons and magnetic fields are expected to be stretched and stirred as a consequence of tangential flows induced by the spiralling gas motion. Lastly, we report on the presence of two thin (6-7 kpc in width) and parallel strands of radio emission embedded in the outer arc that are morphologically similar to the emerging population of non-thermal filaments observed in galaxy clusters, radio galaxies, and the Galactic centre. While this work further demonstrates the complex interplay between thermal and non-thermal components in the intracluster medium, follow-up observations in radio and X-rays are required to firmly determine the origin of the features observed in Abell 2657.Comment: 12 pages, 9 figures, 1 table. Accepted for publication in MNRA