A warrior’s journey? Some recent taxonomic, trace element and lead isotope analyses of Mediterranean later Bronze Age metalwork in the Central and West Balkans

The question of mobility between the different geographic and cultural regions of the Balkans, Aegean and Central Mediterranean remains an issue of crucial importance for understanding the development of Bronze Age societies in each region. While mobility can take many forms, in this paper we are particularly concerned with the movement of personal objects as a means to explore personal mobility. To do this, the paper brings together typological, trace element and lead isotope analyses and the role of portable XRF in this process is explored. It is concluded that a multiproxy analyses that includes typologies can offer valuable insights into the nature and directionality of mobility

Conservative Management of Paediatric Clavicle Fractures

Paediatric clavicle fractures have traditionally been treated nonoperatively. Recent studies have recommended operative management for displaced midshaft fractures. We conducted a retrospective review of all clavicle fractures in children aged one to sixteen over a two-year period. We classified fractures and evaluated followup and clinical outcome. We identified 190 fractures. There were 135 boys and 55 girls. 65% of fractures were displaced and 35% undisplaced. Mean radiographic and clinical followup was 35 days and 44 days, respectively. Clavicle fractures in children heal with nonoperative management. Radiographs of clavicle fractures in children are unnecessary in the absence of clinical symptoms

Alkaloids in Australian rye ergot (Claviceps purpurea) sclerotia: implications for food and stockfeed regulations

Rye ergot (Claviceps purpurea) occasionally causes toxicity (chiefly expressed as hyperthermia) in Australian livestock, either as a result of grazing infected annual (Lolium rigidum) and perennial (L. perenne) rye grasses, or if the ergot sclerotia produced in rye grasses contaminate grain crops used as stockfood. Alkaloids in 30 samples of Australian rye ergot sclerotia taken from rye grasses and grain screenings, and some feed samples contaminated with rye grass ergot sclerotia, were assayed by high performance liquid chromatography. Samples originated from across southern Australia. Ergotamine was the dominant alkaloid in all samples, followed by α-ergocryptine, ergocornine, ergosine and their respective -imine epimers. Ergotamine concentrations in sclerotia ranged up to 2257 mg/kg (as received basis). Ergocristine was a very minor component (<50 mg/kg) in all samples. Total alkaloids in freshly collected sclerotia ranged from 1003 to 3321 mg/kg (0.10 to 0.33%), and up to 3766 mg/kg with epimers included, although lower concentrations were found in samples stored for some time. Alkaloid profiles in sclerotia were all very similar, and concentrations did not appear to be related to size of sclerotia, source region, nor to the rye grass or grain from which they were taken. Previous cases of toxicity in livestock are reviewed and several new cases are reported. The implications of variable alkaloid contents of rye ergot sclerotia are discussed in terms of Australian food and stockfeed regulations

Exploring the efficacy of comparative bioarchaeological approaches in providing answers on marginality and networking:The example of Late Bronze Age Achlada in Florina, Northern Greece

This paper investigates the efficacy of comparative bioarchaeological approaches in exploring the impact of economic marginality on human lifeways. Skeletal remains from the Late Bronze Age cemetery of Achlada in Northern Greece were chosen to address this, as this specific community was probably less well networked, evident in its location away from major communication routes and the paucity of grave goods at the site. A biocultural methodology combining comparative data on funerary practices and lifestyle was implemented. Sex differences were found within the community and seem to agree with the differential burial placement of the sexes possibly representing the different roles that society symbolically attributed to men and women in deathways. Comparative intercemetery data did not reveal poorer health and diet, or more intense physical activity, compared to well-networked sites. Nonetheless, Achlada, as well as numerous, mostly north communities of the wider context, probably faced more physiological challenges during growth, at least of a mild to moderate level, compared to a number of populations connected by major communication routes. The current study highlights the importance of implementing comparative bioarchaeological approaches as a means of identifying the impact of marginality on human lifeways, particularly in settings with limited material culture information.Limitations linked to preservation issues and the multifactorial nature of lifestyle indicators could be dealt with by future biomolecular and isotopic analyses. Η παρούσα εργασία έχει στόχο να διερευνήσει το κατά πόσο οι συγκριτικές βιοαρχαιολογικές προσεγγίσεις είναι εφικτό να δώσουν απαντήσεις ως προς την επίδραση της οικονομικής περιθωριοποίησης στον ανθρώπινο τρόπο διαβίωσης. Επιλέχθηκαν σκελετικά κατάλοιπα της Ύστερης Εποχής Χαλκού από το νεκροταφείο της Αχλάδας στη Βόρεια Ελλάδα ώστε να απαντηθεί το εν λόγω ερώτημα, καθώς η συγκεκριμένη κοινωνία -βάσει της θέσης της μακριά από τα μεγάλα δίκτυα επικοινωνίας και της παρουσίας πολύ λίγων ταφικών ευρημάτων-ήταν πιθανώς λιγότερο καλά δικτυωμένη. Ακολουθήθηκε μια συνδυαστική βιοπολιτισμική προσέγγιση συγκριτικών ταφικών δεδομένων και συγκριτικών αποτελεσμάτων δεικτών τρόπου διαβίωσης. Βρέθηκαν διαφορές μεταξύ των δύο φύλων στον εν λόγω πληθυσμό οι οποίες φαίνεται να συμφωνούν με την διαφορετική πλευρά κατάκλισής τους, η οποία πιθανώς να συμβόλιζε τη διαφορετικότητα των ρόλων που η κοινωνία απέδιδε σε άντρες και γυναίκες στο ταφικό περιβάλλον. Συγκριτικά αποτελέσματα μεταξύ νεκροταφείων δεν φανέρωσαν χαμηλότερο επίπεδο υγείας και διατροφής, ούτε πιο έντονη εργασιακή καταπόνηση, σε σχέση με καλά δικτυωμένες θέσεις. Παρόλα αυτά, η Αχλάδα, όπως και μια σειρά –κυρίως βόρειων- κοινωνιών τουευρύτερου πλαισίου, πιθανότητα αντιμετώπισαν περισσότερα φαινόμενα καταπόνησης (στρες) κατά τη διάρκεια της ανάπτυξης, τουλάχιστον ήπιου και μετρίου επιπέδου, συγκριτικά με καλύτερα δικτυωμένους πληθυσμούς. Η παρούσα εργασία τονίζει τη σημασία της εφαρμογής συγκριτικών βιοαρχαιολογικών προσεγγίσεων ως μέσο μελέτης της επίδρασης της περιθωριοποίησης στους ανθρώπινους πληθυσμούς, ιδιαιτέρως σε θέσεις με περιορισμένες πληροφορίες υλικού πολιτισμού. Μεθοδολογικοί περιορισμοί οι οποίοι συνδέονται με ζητήματα διατήρησης αλλά και με τον πολυπαραγοντικό χαρακτήρα των δεικτών τρόπου διαβίωσης, ενδεχομένως να αντιμετωπιστούν μέσω των επερχόμενων βιομοριακών και ισοτοπικών αναλύσεων.European Commission Horizon 202

Replication and exploratory analysis of 24 candidate risk polymorphisms for neural tube defects.

BackgroundNeural tube defects (NTDs), which are among the most common congenital malformations, are influenced by environmental and genetic factors. Low maternal folate is the strongest known contributing factor, making variants in genes in the folate metabolic pathway attractive candidates for NTD risk. Multiple studies have identified nominally significant allelic associations with NTDs. We tested whether associations detected in a large Irish cohort could be replicated in an independent population.MethodsReplication tests of 24 nominally significant NTD associations were performed in racially/ethnically matched populations. Family-based tests of fifteen nominally significant single nucleotide polymorphisms (SNPs) were repeated in a cohort of NTD trios (530 cases and their parents) from the United Kingdom, and case-control tests of nine nominally significant SNPs were repeated in a cohort (190 cases, 941 controls) from New York State (NYS). Secondary hypotheses involved evaluating the latter set of nine SNPs for NTD association using alternate case-control models and NTD groupings in white, African American and Hispanic cohorts from NYS.ResultsOf the 24 SNPs tested for replication, ADA rs452159 and MTR rs10925260 were significantly associated with isolated NTDs. Of the secondary tests performed, ARID1A rs11247593 was associated with NTDs in whites, and ALDH1A2 rs7169289 was associated with isolated NTDs in African Americans.ConclusionsWe report a number of associations between SNP genotypes and neural tube defects. These associations were nominally significant before correction for multiple hypothesis testing. These corrections are highly conservative for association studies of untested hypotheses, and may be too conservative for replication studies. We therefore believe the true effect of these four nominally significant SNPs on NTD risk will be more definitively determined by further study in other populations, and eventual meta-analysis

Folate status assessment history: implications for measurement of biomarkers in NHANES12345

This article presents a historical perspective on the different methods used to measure folate status in populations and clinical settings. I discuss some of the advantages and limitations of these procedures. For >50 y researchers have used microbiological assay methods to assess folate status in clinical settings and in population-based studies, such as NHANES. Serum and red blood cell folate values obtained with the Lactobacillus casei assay have formed the basis for current ranges and cutoffs for the establishment of folate sufficiency and for the current dietary reference intakes for folate. Over the past 30 y competitive folate protein binding assays, which are available in kit form, have supplanted microbiological assays in many clinical laboratories because of their ease of use. Several NHANES cycles have used these assays. Folate concentrations obtained with these kits are lower than those from microbiological assays and show a wide variation between different protein binding assay kits. This variation has complicated the setting of values for normal ranges of folate status and the comparison of status changes between different NHANES cycles. The recent development of mass spectrometry methods for folate opens up the possibility of measurement of individual folate vitamers such as folic acid. Past experience with microbiological and competitive protein binding assays indicates some of the technical problems that research will need to address before this promise becomes reality

Remission in psoriatic arthritis: is it possible and how can it be predicted?

10.1186/ar3021Arthritis Research and Therapy123R9