1 research outputs found
AMPEROMETRIC BIOSENSORS BASED ON IMMOBILIZED ENZYMES AND CHEMICALLY MODIFIED ELECTRODES
Amperometric biosensors based on two different reaction mechanisms
are presented. Common to both types is the combination of a selective
catalytic reaction that can be followed amperometrically at 0 mV vs
SCE and below.
One type is based on the chemical modification of carbon pastes with
a dehydrogenase, the necessary cofactor NAD’, and a redox mediator.
In the presence of the enzyme substrate NADH will be produced. The
high overvoltage for the electrochemical oxidation of the NADH is
decreased by the addition of the redox mediator to the paste. The
redox mediators used are phenoxazine derivatives making the
electrocatalytic oxidation of NADH possible at 0 mV vs SCE and below.
A glucose sensor based on glucose dehydrogenase is described.
Another type is based on the co-immobilization of a hydrogen
peroxide producing oxidase with horse radish peroxidase on the
surface of heat-treated graphite. The detection is based on an
apparent direct electron transfer from the electrode to the
immobilized peroxidase starting at +600 mV and reaching a maximum at
about O mV vs SCE. The co-immobilized enzyme layer is stabilised by
the addition of bovine serum albumin and glutaraldehyde to the
reaction mixture. A glucose sensor based on glucose oxidase is
presented