Microsatellite Markers Isolated From Cabomba Aquatica Sl (cabombaceae) From An Enriched Genomic Library

Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Premise of the study: Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Methods and Results: Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Virua population. Conclusions: The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.311Post-Graduate program in Plant Biology of the Instituto de Biologia (UNICAMP)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)CAPES [PNADB 457/2010

Microsatellite markers isolated from Cabomba Aquatica S.l. (cabombaceae) from an enriched genomic library

Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Using a selective hybridization method, we designed and tested 30 simple sequence repeat loci using two natural populations of C. aquatica s.l., resulting in 13 amplifiable loci. Twelve loci were polymorphic, and alleles per locus ranged from two to four across the 49 C. aquatica s.l. individuals. Observed heterozygosity, expected heterozygosity, and fixation index varied from 0.0 to 1.0, 0.0 to 0.5, and -1.0 to -0.0667, respectively, for the Manaus population and from 0.0 to 1.0, 0.0 to 0.6, and -1.0 to 0.4643 for the Viruá population. The developed markers will be used in further taxonomic and population studies within Cabomba. This set of microsatellite primers represents the first report on rapid molecular markers in the genus.Microsatellite primers were designed for the submersed aquatic plant Cabomba aquatica s.l. (Cabombaceae) and characterized to estimate genetic diversity parameters. Using a selective hybridization method, we designed and tested 30 simple sequence repeat l31113CNQP - CONSELHO NACIONAL DE DESENVOLVIMENTO CIENTÍFICO E TECNOLÓGICOCAPES - COORDENAÇÃO DE APERFEIÇOAMENTO DE PESSOAL DE NÍVEL SUPERIORSEM INFORMAÇÃO457/2010The authors thank S. M. Costa for help during fieldwork, the staff of the Parque Nacional do Viruá and Instituto Nacional de Pesquisas da Amazônia (INPA) for field support, and A. P. Souza for technical support for the laboratory infrastructure. T.D.M.B.

Analysis of the relationship between perceived organizational support, exhaustion and organizational commitment.

Esta pesquisa teve como objetivo analisar a influ?ncia que o suporte organizacional percebido exerce sobre o n?vel de exaust?o emocional e o comprometimento organizacional dos trabalhadores. Realizou-se um levantamento de campo nas cidades de Mariana e Ouro Preto, Estado de Minas Gerais, Brasil. Para a an?lise de dados, foi utilizada a t?cnica de Modelagem de Equa??es Estruturais. Os resultados encontrados constataram que h? uma rela??o negativa entre o suporte organizacional percebido e a exaust?o do trabalhador, e uma rela??o positiva entre o suporte organizacional percebido e o comprometimento do trabalhador. Tais resultados evidenciam que as organiza??es que se preocupam com o bem-estar do funcion?rio contribuem para o aumento do compromisso e redu??o do esgotamento por ele demonstrados. Al?m disso, verificou-se uma rela??o positiva entre a exaust?o e o comprometimento do trabalhador. Essa ?ltima rela??o foi explicada levantando-se a hip?tese de que os indiv?duos mais esgotados podem se apegar ? organiza??o, esperando que ela reconhe?a e recompense o seu esfor?o no futuro.This research aimed to analyze the influence that the perceived organizational support exerts on the level of emotional exhaustion and the organizational commitment of the workers. A survey was conducted in the cities of Mariana and Ouro Preto, State of Minas Gerais, Brazil. For the data analysis, the Structural Equation Modeling technique was used. The results showed that there is a negative relationship between perceived organizational support and worker exhaustion, and a positive relationship between perceived organizational support and worker commitment. These results show that organizations concerned with the well-being of their employees contribute to the increase of commitment and reduction of their exhaustion. In addition, a positive relationship between worker exhaustion and commitment was identified. This last relation was explained by proposing the hypothesis that the most exhausted individuals can become attached to the organization, hoping that it will recognize and reward its effort in the future

Portuguese propolis antitumoral activity in melanoma involves ROS production and induction of apoptosis

Melanoma is the most aggressive and life-threatening skin cancer type. The melanoma genome is the most frequently mutated, with the BRAF mutation present in 40–60% of melanoma cases. BRAF-mutated melanomas are characterized by a higher aggressiveness and progression. Adjuvant targeted treatments, such as BRAF and MEK inhibitors, are added to surgical excision in BRAF-mutated metastatic melanomas to maximize treatment effectiveness. However, resistance remains the major therapeutic problem. Interest in natural products, like propolis, for therapeutic applications, has increased in the last years. Propolis healing proprieties offer great potential for the development of novel cancer drugs. As the activity of Portuguese propolis has never been studied in melanoma, we evaluated the antitumoral activity of propolis from Gerês (G18.EE) and its fractions (n-hexane, ethyl acetate (EtOAc), and n-butanol) in A375 and WM9 melanoma cell lines. Results from DPPH•/ABTS• radical scavenging assays indicated that the samples had relevant antioxidant activity, however, this was not confirmed in the cell models. G18.EE and its fractions decreased cell viability (SRB assay) and promoted ROS production (DHE/Mitotracker probes by flow cytometry), leading to activation of apoptotic signaling (expression of apoptosis markers). Our results suggest that the n-BuOH fraction has the potential to be explored in the pharmacological therapy of melanoma.Foundation for Science and Technology (FCT)—projects UIDB/04050/2020, UDBI/04033/2020, UIDB/50026/2020, UIDP/50026/2020, UIDB/50006/2020, UIDP/50006/2020, and by the project NORTE-01-0145-FEDER-000055, supported by the Norte Portugal Regional Operational Programme (NORTE 2020), under the PORTUGAL 2020 Partnership Agreement, through the European Regional Development Fund (ERDF). S.P.C., C.B.M. and A.S.F. are recipients of FCT grants (2020.05779.BD, SFRH/BD/145955/2019, and PD/BD/128276/2017, respectively

Sensor Surface Design with NanoMaterials: A New Platform in the Diagnosis of COVID-19

Mass testing for COVID-19 is essential to defining patient management strategies, choosing the best clinical management, and dimensioning strategies for controlling viral dissemination and immunization strategies. Thus, it is of utmost importance to search for devices that allow a quick and reliable diagnosis of low cost that can be transposed from the bench to the bedside, such as biosensors. These devices can help choose the correct clinical management to minimize factors that lead to infected patients developing more severe diseases. The use of nanomaterials to modify biosensors’ surfaces to increase these devices’ sensitivity and their biofunctionality enables high-quality nanotechnological platforms. In addition to the diagnostic benefits, nanotechnological platforms that facilitate the monitoring of anti-SARS-CoV-2 antibodies may be the key to determining loss of protective immune response after an episode of COVID-19, which leads to a possible chance of reinfection, as well as how they can be used to assess and monitor the success of immunization strategies, which are beginning to be administered on a large scale and that the extent and duration of their protection will need to be determined. Therefore, in this chapter, we will cover nanomaterials’ use and their functionalities in the surface design of sensors, thus generating nanotechnological platforms in the various facets of the diagnosis of COVID-19