Genome-wide association analysis identifies 30 new susceptibility loci for schizophrenia

We conducted a genome-wide association study (GWAS) with replication in 36,180 Chinese individuals and performed further transancestry meta-analyses with data from the Psychiatry Genomics Consortium (PGC2). Approximately 95% of the genome-wide significant (GWS) index alleles (or their proxies) from the PGC2 study were overrepresented in Chinese schizophrenia cases, including ∼50% that achieved nominal significance and ∼75% that continued to be GWS in the transancestry analysis. The Chinese-only analysis identified seven GWS loci; three of these also were GWS in the transancestry analyses, which identified 109 GWS loci, thus yielding a total of 113 GWS loci (30 novel) in at least one of these analyses. We observed improvements in the fine-mapping resolution at many susceptibility loci. Our results provide several lines of evidence supporting candidate genes at many loci and highlight some pathways for further research. Together, our findings provide novel insight into the genetic architecture and biological etiology of schizophrenia

Quality Evaluation of Ophiopogon japonicus from Two Authentic Geographical Origins in China Based on Physicochemical and Pharmacological Properties of Their Polysaccharides

Ophiopogon japonicus is widely used as a tonic herb in China. According to the origins, MaiDong of Chinese materia medica can be classified as Zhe MaiDong (Ophiopogon japonicus in Zhejiang), Chuan MaiDong (Ophiopogon japonicus in Sichuan), Duanting Shan MaiDong (Liriope muscari), and Hubei MaiDong (Liriope spicata). In terms of quality control, polysaccharides-based evaluations have not yet been conducted. In this study, microwave-assisted extraction (MAE) was used for the preparation of polysaccharides from 29 batches of MaiDong. HPSEC-MALLS-RID and HPAEC-PAD were employed to investigate their molecular parameters and compositional monosaccharides, respectively. The ability to scavenge ABTS radicals and immune promotion abilities, in terms of nitric oxide releasing and phagocytosis on RAW 264.7 macrophages, were also compared. The results showed that polysaccharides in different MaiDong varied in molecular parameters. All polysaccharides mainly contained fructose and glucose with small amounts of arabinose, mannose, galactose, and xylose. For polysaccharides of Zhe MaiDong and Chuan MaiDong, the molar ratio of Fru to Glc was roughly 15:1 and 14:1, respectively. Zhe MaiDong exhibited better antioxidant and immune promotion activity, and so did that of fibrous roots. The pharmacological activity, however, did not account for the variation in growth years. Finally, indicators for quality control based on multivariate statistical analysis included: yield, antioxidant activity, the content of fructose, and RI signal. It was concluded that MaiDong’s fibrous roots had similar components to the root, and their quality was not significantly affected by growth age. This may provide some guidance for the cultivation and use of MaiDong

Characterization and Comparison of Bioactive Polysaccharides from <i>Grifola frondosa</i> by HPSEC-MALLS-RID and Saccharide Mapping Based on HPAEC-PAD

Grifola frondosa polysaccharides (GFPs) from different regions in China were characterized and compared using HPSEC-MALLS-RID and saccharide mapping based on HPAEC-PAD analysis for achieving and improving its quality control. The results showed that HPSEC chromatograms and molecular weight distributions of GFPs were similar. The average contents of each polysaccharide fraction (Peaks 1, 2, and 3) showed that Peak 3 was the main component and much higher than the other two polysaccharide fractions, which also contained protein. The result of saccharide mapping showed that α-1,4-glycosidic, β-1,4-glycosidic and few β-1,3-glycosidic linkages were existed in GFPs. The similarity result showed that HPAEC-PAD fingerprints of the oligosaccharide fragments after hydrolysis by endoglycosidase were certainly different, especially α-amylase with a mean similar index of only 0.781 ± 0.207. The result of hierarchical cluster analysis (HCA) showed that different batches of GFPs from China can be divided into different clusters. Furthermore, immune-enhancing activity based on RAW 264.7 cells showed significant differences among different GFPs. Based on grey relational analysis (GRA), the fractions of Peak 3 were regarded as the major contributors to its immuno-enhancing activity in GFPs. Overall, the implications from these results were found to be stable, comprehensive, and valid for improving the quality control of GFPs

Numerical Investigation of the Effect of Hub Gaps on the 3D Flows Inside the Stator of a Highly Loaded Axial Compressor Stage

Both the compressor performance and the 3D flows inside the stator passage are significantly impacted by the stator hub gap. The interplay between leakage flow and corner separation within a cantilevered stator of a highly loaded, low-speed axial compressor with a succession of stator hub gaps was examined numerically in this paper. Firstly, the simulated results were compared with the measured results, including the compressor characteristics, the 3D flow structures, and the flow fields at the stator outlet. The results revealed that the used CFD solver, as well as the corresponding setup, can reproduce the flow not only in terms of the trend along with the stator hub gap, but also in terms of the specific scale of the 3D flow structure. Hence, it is feasible enough to be applied in the present investigation. Secondly, the flow mechanisms of the interplay between the corner separation and the leakage flow with different stator hub gaps were analyzed. It was found that the velocity of the leakage flow is the key parameter that dominates the flow structures as well as the compressor performance. Additionally, a simple metric was proposed to be used to choose the optimum stator hub gap. By comparing our results with those from published research, this metric was proven to be feasible. Finally, it is also discussed how the stator hub gap affected the stator inlet flow and rotor performance. It is demonstrated that the stator passage flow blockage can affect the upstream flow field. As a result, the performance of the rotor tends to vary in the opposite direction to that of the stator

Guizhi Fuling Capsule Exhibits Antidysmenorrhea Activity by Inhibition of Cyclooxygenase Activity

Guizhi Fuling capsule (GZFLc) is a modern preparation from traditional Chinese Medicine. Guizhi Fuling was first prescribed by Zhang Zhongjing almost two thousand years ago for the treatment of primary dysmenorrhea. It has also been used to treat uterine fibroids, dysfunctional uterine bleeding, and endometriosis. Although effective against dysmenorrhea clinically, there are limited information on the mechanism of its action. The major components responsible for the activity are not well defined. The aim of this study has been to elucidate a mechanism that may facilitate the development of a bioactivity-based assay for quality control during drug formulation and manufacturing. Using an oxytocin-induced mouse dysmenorrhea model, we showed that oral administration of GZFLc at 150 and 300 mg/kg, dosages relevant to clinic usages, significantly suppressed oxytocin-induced writhing response. The antidysmenorrhea effect was also demonstrated by a rotarod assay. We showed that GZFLc treatment significantly prolonged the hanging time of mice on the rotating rod. Histological studies showed that GZFLc treatment reduced lamina propria edema, while no effect on COX2 expression was detected. GZFLc instead exhibited direct inhibitory effect against COX2, a critical enzyme that catalyzes arachidonic acid conversion to prostaglandins. By HPLC profiling, we showed that paeoniflorin, paeonol, and cinnamaldehyde are the major components from the corresponding plants. At 5 and 10 mg/kg, both paeoniflorin and paeonol were active against induced dysmenorrhea. The study not only links GZFLc antidysmenorrhea activity to COX2 inhibition but also uncovers a mechanism of action by which an assay can be developed for bioefficacy evaluation of GZFLc

Heterojunction solar cells with asymmetrically carrier-selective contact structure of molybdenum-oxide/silicon/magnesium-oxide

New functional materials that are constantly introduced into carrier-selective contacts (CSCs), which allow one type of carrier passing through while blocking the other type via energy band alignment at contact region, promote the fabrication of crystalline silicon (c-Si) solar cells towards low-temperature and dopant-free. Here, electron-beam-evaporated molybdenum oxide (MoOx) and magnesium oxide (MgOx) are directly deposited upon the front and rear surface of c-Si substrates, respectively, to form CSCs with asymmetric band offset for holes and electrons. Contact resistivity, passivation effect, interfacial structures and chemical states for both MoOx/c-Si and MgOx/c-Si are systematically characterized. Considering good carrier-selectivity at the front and the rear side, the optimum thickness in terms of contact resistivity and photovoltaic performance is 10 nm for MoOx and 1.5 nm for MgOx, respectively. Finally, an efficiency over 14% for the planar MoOx/c-Si/MgOx heterojunction solar cells is achieved, demonstrating huge economic potential in fabrication procedure over conventional high temperature diffused homojunction solar cells

Engineering of hole-selective contact for high-performance perovskite solar cell featuring silver back-electrode

Engineering of hole-selective contact for high-performance perovskite solar cell featuring silver back-electrod

Breeding and Agronomic Evaluation of Jilv 20, a New Mungbean (Vigna radiata L.) Cultivar

Mungbean (Vigna radiata L.), one of the most widely grown edible legumes in Asia, plays important roles in the improvement of agricultural cultivation systems as well as human diets. As an understudied crop, however, most mungbean varieties are characterized by unstable yields, poor disease resistance, and unsuitability for mechanical harvesting, thereby leading to a low production income. We, therefore, developed Jilv 20, a new mungbean cultivar with a dull seed coat, by crossing Bao 942-34 with Weilv 9002-341. After 11 years of selection and evaluation, Jilv 20 has shown wide adaptability, early maturity, high yield, halo blight resistance, and suitability for mechanical harvesting. The growth period of Jilv 20 is 79.5 and 66.7 days in the spring and summer sowing seasons, respectively, and the average plant height is approximately 56.4 cm. In this study, average yields were 1737.9, 1532.3, and 2045.1 kg/hm2 in northern spring-sowing, northern summer-sowing, and southern sites, respectively; these values were respectively 27.83%, 28.48%, and 6.96% higher than those of control cultivar Zhonglv 5, which has been popular in past decades because of its wide adaptability. The average protein and starch contents of Jilv 20 seeds were 25.0% and 49.56%, respectively. Further application and extension of Jilv 20 in China should contribute to mungbean production, breeding, and industrial development