3,6,8-Tribromo-7-ethyl­amino-4-methyl-2H-chromen-2-one

In the title mol­ecule, C12H10Br3NO2, the 2H-chromen ring is essentially planar (r.m.s. deviation = 0.022 Å) with the ethyl­amino group oriented at 13.9 (5)° with respect to the ring. The mol­ecular structure is stabilized by intra­molecular N—H⋯Br and C—H⋯Br interactions

3,6-Dibromo-7-ethyl­amino-4-methyl-2H-chromen-2-one

In title compound, C12H11Br2NO2, the coumarin ring system is almost planar, the two rings being inclined to one another by 1.40 (15)°. There are two short intra­molecular inter­actions (N—H⋯Br and C—H⋯Br) involving the Br atoms. In the crystal, mol­ecules stack along the a-axis direction via π–π inter­actions; the centroid–centroid distances vary from 3.6484 (19) to 3.7942 (19) Å

A pilot study on acute inflammation and cancer: a new balance between IFN-γ and TGF-β in melanoma

Recent data have redefined the concept of inflammation as a critical component of tumor progression. However, there has been little development on cases where inflammation on or near a wound and a tumor exist simultaneously. Therefore, this pilot study aims to observe the impact of a wound on a tumor, to build a new mouse tumor model with a manufactured surgical wound representing acute inflammation, and to evaluate the relationship between acute inflammation or wound healing and the process of tumor growth. We focus on the two phases that are present when acute inflammation influences tumor. In the early phase, inhibitory effects are present. The process that produces these effects is the functional reaction of IFN-γ secretions from a wound inflammation. In the latter phase, the inhibited tumor is made resistant to IFN-γ through the release of TGF-β to balance the inflammatory factor effect on the tumor cells. A pair of cytokines IFN-γ/TGF-β established a new balance to protect the tumor from the interference effect of the inflammation. The tumor was made resistant to IFN-γ through the release of TGF-β to balance the inflammatory effect on the tumor cells. This balance mechanism that occurred in the tumor cells increased proliferation and invasion. In vitro and in vivo experiments have confirmed a new view of clinical surgery that will provide more detailed information on the evaluation of tumors after surgery. This study also provides a better understanding of the relationship between tumor and inflammation, as well as tumor cell attacks on inflammatory factors

Graphene oxide nanoparticles for enhanced photothermal cancer cell therapy under the irradiation of a femtosecond laser beam

Nano-sized graphene and graphene oxide (GO) are promising for biomedical applications, such as drug delivery and photothermal therapy of cancer. It is observed in thiswork that the ultrafast reduction of GO nanoparticles (GONs)with a femtosecond laser beam creates extensive microbubbling. To understand the surface chemistry of GONs on the microbubble formation, the GONs were reduced to remove most of the oxygen-containing groups to get reduced GONs (rGONs). Microbubbling was not observed when the rGONs were irradiated by the laser. The instant collapse of the microbubbles may produce microcavitation effect that brings about localized mechanical damage. To understand the potential applications of this phenomenon, cancer cells labeled with GONs or rGONs were irradiated with the laser. Interestingly, the microbubbling effect greatly facilitated the destruction of cancer cells. When microbubbles were produced, the effective laser power was reduced to less than half of what is needed when microbubbling is absent. This finding will contribute to the safe application of femtosecond laser in the medical area by taking advantage of the ultrafast reduction of GONs. It may also find other important applications that need highly localized microcavitation effects

Determination of Five Synthetic Colorants in Grain Food by High Performance Liquid Chromatography-tandem Mass Spectrum

A new application of baking soda was developed, the synthetic colorant in cereal was extracted by adding with methanol solution in this paper.To develop new uses of edible bicarbonate,synthetic colorants were extracted from cereals by adding methanol water solution of edible bicarbonate in this paper, Ultra-high performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)was used to test standard recovery.The extraction effect of edible bicarbonate of soda on five synthetic colorants of sunset yellow, amaranth red, carmine red, alluvium red and bright blue in cereals was investigated.The sample was extracted with 70 mL 40% methanol water vortexed with 1g edible baking soda, purified with Strata X-AW SPE column and determined.The results showed that, a good linearity was observed between 0.1 and 1.4μg/mL, and for the 5 synthetic colorants, the limit of detection was between 0.07 and 0.50 μg/g.The recoveries of the 5 synthetic colorants of different food substrates at different spiked levels (1, 2 and 10 μg/g) were all found to be in the recovery range specified in GB/T27404.The recoveries for the 5 synthetic colorants were found between 80.0% and 110% with RSD from 0.7% to 5.1% (n=6).Hence, baking soda added to 40% methanol water solution can be used to extract synthetic colorants from grain products with simple operation, accurate results, high efficiency and environmental protection

Association between acute kidney injury and prognoses of cardiac surgery patients: Analysis of the MIMIC-III database

BackgroundAcute kidney injury (AKI) is the most common major complication of cardiac surgery field. The purpose of this study is to investigate the association between acute kidney injury and the prognoses of cardiac surgery patients in the Medical Information Mart for Intensive Care III (MIMIC-III) database.MethodsClinical data were extracted from the MIMIC-III database. Adult (≥18 years) cardiac surgery patients in the database were enrolled. Multivariable logistic regression analyses were employed to assess the associations between acute kidney injury (AKI) comorbidity and 30-day mortality, 90-day mortality and hospital mortality. Different adjusting models were used to adjust for potential confounders.ResultsA total of 6,002 patients were involved, among which 485 patients (8.08%) had comorbid AKI. Patients with AKI were at higher risks of prolonged ICU stay, hospital mortality, 90-day mortality (all P < 0.001), and 30-day mortality (P = 0.008). AKI was a risk factor for hospital mortality [Model 1, OR (95% CI) = 2.50 (1.45–4.33); Model 2, OR (95% CI) = 2.44 (1.48–4.02)], 30-day mortality [Model 1, OR (95% CI) = 1.84 (1.05–3.24); Model 2, OR (95% CI) = 1.96 (1.13–3.22)] and 90-day mortality [Model 1, OR (95% CI) = 2.05 (1.37–3.01); Model 2, OR (95% CI) = 2.76 (1.93–3.94)]. Higher hospital mortality, 30-day mortality and 90-day mortality was observed in higher KDIGO grade for cardiac surgery patients with AKI (all P < 0.05).ConclusionComorbid AKI increased the risk of hospital mortality, 30-day mortality, and 90-day mortality of cardiac surgery patients in the MIMIC-III database