Aquatic ecosystems in Byers Peninsula, Livingston Island. An Antarctic Noah’s Ark in a changing world

第6回極域科学シンポジウム[OB] 極域生物圏11月17日（火）　統計数理研究所 セミナー室1（D305

Integrin linked kinase (ILK) regulates podosome maturation and stability in dendritic cells

AbstractPodosomes are integrin-based adhesions fundamental for stabilisation of the leading lamellae in migrating dendritic cells (DCs) and for extracellular matrix (ECM) degradation. We have previously shown that soluble factors and chemokines such as SDF 1-a trigger podosome initiation whereas integrin ligands promote podosome maturation and stability in DCs. The exact intracellular signalling pathways that regulate the sequential organisation of podosomal components in response to extracellular cues remain largely undetermined. The Wiskott Aldrich Syndrome Protein (WASP) mediates actin polymerisation and the initial recruitment of integrins and associated proteins in a circular configuration surrounding the core of filamentous actin (F-actin) during podosome initiation. We have now identified integrin linked kinase (ILK) surrounding the podosomal actin core. We report that DC polarisation in response to chemokines and the assembly of actin cores during podosome initiation require PI3K-dependent clustering of the Wiskott Aldrich Syndrome Protein (WASP) in puncta independently of ILK. ILK is essential for the clustering of integrins and associated proteins leading to podosome maturation and stability that are required for degradation of the subjacent extracellular matrix and the invasive motility of DCs across connective tissue barriers.We conclude that WASP regulates DCs polarisation for migration and initiation of actin polymerisation downstream of PI3K in nascent podosomes. Subsequently, ILK mediates the accumulation of integrin-associated proteins during podosome maturation and stability for efficient degradation of the subjacent ECM during the invasive migration of DCs

RiSCC Manual Version 2.0

Regional Sensitivity to Climate Change in Antarctic Terrestrial and Limnetic Ecosystems (RiSCC) is an international research program on Antarctic terrestrial and limnetic organisms and ecosystems. It is sponsored by the Scientific Committee on Antarctic Research (SCAR), a committee of the International Council of Science (ICSU). The RiSCC programme was formally adopted as a research programme under the auspices of the SCAR Working Group on Biology during SCAR XXVI, in Tokyo, Japan, July 2000. A recommendation to approve of this programme was made to the Delegates Meeting of SCAR XXVI. The Delegates agreed to this recommendation. In this manual the agreed methods are being described, which are to be used in RiSCC projects. Methods presented here have been tried in polar regions under extreme conditions, or with organisms obtained from high latitudes, and have been considered appropriate, providing reliable data

Animalité

Ce numéro double du Portique est exclusivement consacré à un dossier conçu par Andrea Potestà sur le thème de l'animalité. Exceptionnellement, cette livraison ne comporte pas de résumé, ni nos rubriques habituelles : Marges et Controverses et Recensions. On retrouvera ces rubriques dans le prochain numéro consacré à " L'architecture des Milieux ". Ce numéro est publié avec le concours du CNL (Centre National du Livre). La direction et la rédaction remercient le CNL pour son aide et ses encouragements

Animalité

Ce numéro double du Portique est exclusivement consacré à un dossier conçu par Andrea Potestà sur le thème de l'animalité. Exceptionnellement, cette livraison ne comporte pas de résumé, ni nos rubriques habituelles : Marges et Controverses et Recensions. On retrouvera ces rubriques dans le prochain numéro consacré à " L'architecture des Milieux ". Ce numéro est publié avec le concours du CNL (Centre National du Livre). La direction et la rédaction remercient le CNL pour son aide et ses encouragements