On the inexplicability of the implicit: differences in the information provided by implicit and explicit tests

Implicit measures are often preferred to overt questioning in many areas of psychology. Their covert nature allows them to circumvent conscious expectations and biases, theoretically providing more objective indicators of people's true attitudes and bel iefs. However, we argue that implicit and explicit measures tap into different memory systems, so that the interpretation of implicit measures is not as straightforward as the interpretation of explicit measures. We conducted an experiment investigating the relation between implicit and explicit measures of person impressions. The results demonstrate that a single stimulus can have opposite effects on implicit and explicit measures, supporting the theory that the measures reflect the contents of different memory systems. We suggest that implicit measures reflect simple associations stored in a "slow-learning" memory system, while explicit measures reflect a combination of these associations with contextually dependent memories stored in a "fast-binding" memory system

From Bjorken Scaling to pQCD--Experimental techniques from p-p collisions of the 1970's with application to Au+Au collisions at RHIC

Hard scattering in p-p collisions was discovered at the CERN ISR in 1972, by the method of leading particles, which proved that the partons of Deeply Inelastic Scattering strongly interacted with each other. Further ISR measurements utilizing inclusive single or pairs of hadrons established that high pT particles are produced from states with two roughly back-to-back jets which are the result of scattering of constituents of the nucleons as described by Quantum Chromodynamics. These techniques, which are the only practical method to study hard-scattering and jet phenomena in Au+Au collisions at RHIC, will be reviewed.Comment: 10 pages, 6 figures, Proceedings of the 18th International Nuclear Physics Divisional Conference of the European Physical Society (NPDC18) Prague, Czech Republic, August 22-28, 2004, to appear in NP

The dual phosphodiesterase 3 and 4 inhibitor RPL554 stimulates CFTR and ciliary beating in primary cultures of bronchial epithelia

Cystic fibrosis (CF), a genetic disease caused by mutations in the CFTR gene, is a life-limiting disease characterized by chronic bacterial airway infection and severe inflammation. Some CFTR mutants have reduced responsiveness to cAMP/PKA signaling; hence, pharmacological agents that elevate intracellular cAMP are potentially useful for the treatment of CF. By inhibiting cAMP breakdown, phosphodiesterase (PDE) inhibitors stimulate CFTR in vitro and in vivo. Here, we demonstrate that PDE inhibition by RPL554, a drug that has been shown to cause bronchodilation in asthma and chronic obstructive pulmonary disease (COPD) patients, stimulates CFTR-dependent ion secretion across bronchial epithelial cells isolated from patients carrying the R117H/F508del CF genotype. RPL554-induced CFTR activity was further increased by the potentiator VX-770, suggesting an additional benefit by the drug combination. RPL554 also increased cilia beat frequency in primary human bronchial epithelial cells. The results indicate RPL554 may increase mucociliary clearance through stimulation of CFTR and increasing ciliary beat frequency and thus could provide a novel therapeutic option for CF

A new enzyme-linked immunosorbent assay to measure anti-endothelial antibodies after cardiac transplantation demonstrates greater inhibition of antibody formation by tacrolimus compared with cyclosporine.

Objective: The transfer of recombinant genes to donor organs may allow for novel therapeutic approaches to the challenges of acute and chronic rejection. Adenoviral vectors are capable of efficient gene transfer, but use of these vectors during donor organ preservation may be less efficient due to the low temperature. This study was designed to examine the effect of temperature on the efficiency of adenovirus-mediated gene transfer. Methods: Gene transfer to human endothelial cells, porcine vascular smooth muscle cells and cultured rat thoracic aortas was examined. Incubation with an adenoviral vector encoding for E. coli beta-galactosidase was performed for 1 h at three different temperatures: 4 degrees C, 10 degrees C and 37 degrees C. Transgene expression was assessed by histochemical staining for beta-galactosidase in transduced cells and by evaluation of beta-galactosidase activity in organ cultures. Results: Both in human endothelial cells and vascular smooth muscle cells the percentage of positively staining cells following transduction at 37 degrees C was significantly greater than at 4 degrees C and at 10 degrees C (30.55 +/- 7.26% vs. 14.29 +/- 3.79% and 12.43 +/- 2.47%, respectively for endothelial cells, P < 0.01 vs. 4 degrees C and 10 degrees C; and 28.25 +/- 4.52% vs. 17.91 +/- 3.76% and 16.63 +/- 3.92%, respectively for smooth muscle cells, P < 0.05 vs. 4 degrees C, P < 0.01 vs. 10 degrees C). beta-galactosidase activity was significantly greater in aortas transduced at 37 degrees C than in vessels transduced at 4 degrees C and 10 degrees C (289700 +/- 113300 vs. 149600 +/- 54390 and 108800 +/- 23140 relative chemiluminesce units/mg of total protein, respectively; P < 0.05 vs. 4 degrees C, P < 0.001 vs. 10 degrees C). Conclusions: The present study demonstrates that the efficiency of adenovirus-mediated gene transfer is significantly reduced at lower temperatures. The need for cold preservation of donor organs may render efficient adenovirus-mediated gene transfer more difficult in the transplantation setting. (C) 1998 Elsevier Science B.V. All rights reserved

Diagnostics of Shiva Nova produced high yield thermonuclear events

Experiments with the Shiva Nova laser facility which produce yield levels of scientific breakeven and above will result in neutron, x-ray and particle fluxes which will require specific attention to the survivability of diagnostic instrumentation. These yield levels will also allow the utilization of new diagnotics techniques which can provide detailed information on the state of the imploded fuel and pusher shells

A phage-displayed mimotope inhibits tumour necrosis factor-α-induced cytotoxicity more effectively than the free mimotope

A phage-displayed peptide library was screened by direct interaction with human tumour necrosis factor-α (TNF-α) to identify novel antagonistic molecules of its biological activities. After several rounds of affinity selection, a phage displaying a mimotope sequence was shown to strongly inhibit, in a dose-dependent fashion, both mouse and human TNF-α-mediated cytotoxicity in L929 cells. The identified mimotope did not bear any sequence homology to the primary structures of the extracellular domains of either the 55 000 MW or the 75 000 MW TNF-α receptors, suggesting that it represents or mimics a conformational epitope involved with binding to TNF-α. The free 15-mer mimotope weakly inhibited TNF-α-induced cytotoxicity in vitro, and it did not bind to TNF-α as assessed by surface plasmon resonance, demonstrating the importance of mimotope presentation for its biological activities. In conclusion, this study highlights the potential of random combinatorial peptide libraries for the identification of novel inhibitors, which may serve as important tools in research that could lead to the development of TNF-α antagonists with therapeutic potential