Synthesis and Optical Properties of Highly Stabilized Peptide- Coated Silver Nanoparticles

The interaction between the silver nanoparticle and peptide surfaces has been of increased interest for the applications of bionanotechnology and tissue engineering. In order to completely understand such interactions, we have examined the optical properties of peptide-coated silver nanoparticles. However, the effect of peptide binding motif upon the silver nanoparticles surface characteristics and physicochemical properties of these nanoparticles remains incompletely understood. Here, we have fabricated sodium citrate stabilized silver nanoparticles and coated with peptide IVD (ID3). The optical properties of these peptide-capped nanomaterials were characterized by UV-visible, transmission electron microscopy (TEM), and z-potential measurement. The results indicate that the interface of silver nanoparticles (AgNP)-peptide is generated using ID3 peptide and suggested that the reactivity of peptide is governed by the conformation of the bound peptide on the silver nanoparticle surface. The interactions of peptide-nanoparticle would potentially be used to fabricate specific functionality into the various peptide-capped nanomaterials and antibacterial applications

Engineering micro/nano-fibrous scaffolds with silver coating for tailored wound repair applications

Electrospun scaffolds originating from polymeric amalgams, specifically poly(glycerol sebacate/poly(ε-caprolactone) (PGS/PCL) and poly(methyl methacrylate)–poly(ε-caprolactone) (PMMA/PCL), have emerged as a versatile substrate within the realm of biomedical tissue engineering. Their salience is underscored by their remarkable thermal, optical, and mechanical attributes. In this investigation, we harnessed conventional electro-spinning methodologies to fabricate nano/micro-fibrous scaffolds from a hybrid composite, amalgamating PMMA/PCL and PGS/PCL fibers. A pivotal innovation lay in the precise deposition of silver nanoparticles (AgNPs) on one facet of these scaffolds, endowing them with anti-bacterial functionality. This AgNP coating not only forestalled melting proclivities but also meticulously tuned structural facets, engendering a diminution in pore diameter and augmentation in fiber diameter, thereby engendering an elevation in thermo-mechanical performance. Comparative scrutiny delineated that the PMMA/PCL composite fibrous scaffolds manifested superior mechanical attributes, including augmented modulus (E) and ultimate tensile strength (UTS), accompanied by attenuated tensile strain, obviating the requisite for supplementary post-processing steps. These AgNP-endowed composite fibrous scaffolds engender sanguine prospects for biomedical applications, encompassing surgical meshes, bandages, and band-aids, underpinned by their amplified anti-bacterial characteristics, which are instrumental in the context of wound healing

Facile Synthesis of Silver Nanoparticles Using Green Tea Leaf Extract and Evolution of Antibacterial Activity

The scientific society is exploiting the use of nanoparticles in nano-medicine and biomedical applications. In the field of biomaterial and bio-nanotechnology, silver nanoparticles (AgNPs) are playing an important role due to their potential physical, chemical, and biological properties ranging in activities from antibacterial, antiviral, antifungal, and anticancer treatment. Green synthesis technology is one of the most cost-effective, eco-friendly, and biologically safe methods. Green tea leaf extract can reduce silver to AgNPs and enhance antibacterial activity. In this work, we demonstrate the antibacterial activity effect employing green synthesis of AgNPs with green tea leaf extract. The UV–Vis and FTIR results showed, confirming the formation of AgNPs and the presence of chemical groups enhancing the antibacterial activity of AgNPs. The synthesized AgNPs with green tea leaf extract were crystalline with a quasi-spherical shape with a diameter from 30 to 150 nm. The antibacterial activity of the AgNPs in three different concentrations showed that the 120 mg/ml sample possesses higher antibacterial activity (significantly high killing ability) against E. coli than chemically produced AgNPs. These results confirm a more significant antibacterial effect of the biogenic AgNPs with low cytotoxicity than the AgNPs produced chemically. These findings can be used to treat chronic infections, diseases, and other biomedical applications. Graphical Abstract: [Figure not available: see fulltext.]