Effect of Cultivars and Season on Grafting Success in Sapota under Paschim Midnapur Conditions of West Bengal

Two sets of experiments were carried out during 2007-08 to assess incompatibility of sapota cultivars to softwood grafting, and to find out the best time for softwood grafting, in a private orchard at Jhargram of Paschim Midnapore, West Bengal. Considerable variation in success of softwood grafting among sapota cultivars was observed. Among ten cultivars studied, CO-2 showed highest compatibility with Khirnee rootstock to softwood grafting, followed by Cricket Ball and DSH-2. There was a total failure in graft-take in cultivars CO-1, DSH-1 and Guthi. Softwood grafting success was highest in sapota when carried out on 1stJuly (72%) followed by 15th August (70%), 5th June (62%) and 15th June (56%)

A fluorescence anisotropy study of tetramer-dimer equilibrium of λ repressor and its implication for function

Tetramer-dimer equilibrium of λ repressor has been studied by fluorescence anisotropy techniques. We have chosen 1-dimethylamino naphthalene-5-sulfonyl chloride (dansyl chloride)-labeled repressor to study the dissociation-association equilibrium, because of relatively long life-time of the probe (> 10 ns). Polarization of the dansyl-labeled repressor decreases with decreasing protein concentrations in the range of 20 to 0.2 μM. The decrease of anisotropy was shown to be due to reversible dissociation of the protein. Size exclusion high-performance liquid chromatography studies and polyacrylamide gel electrophoresis under native conditions (Ferguson plot) confirmed that at around 20 μM concentrations the repressor exists in predominantly tetrameric form, whereas in lower concentrations it exists in predominantly dimer form. A dissociation constant of 2.3 ± 0.9 μM was estimated in 0.1 M potassium phosphate, pH 8.0, at 25° C. A stoichiometric amount of isolated single operator shifted the tetramer-dimer equilibrium toward the dimer. Increased ionic strength had only a modest effect on the dissociation constant. The thermodynamic constants for the dissociation reaction calculated from the Van't Hoff plot was +26.6 kcal/mol for ΔH and +64.7 e.u. for ΔS. The rotational correlation times derived from isothermal Perrin plot indicated elongated dimers and tetramers

Sequence sensitivity of breathing dynamics in heteropolymer DNA

We study the fluctuation dynamics of localized denaturation bubbles in heteropolymer DNA with a master equation and complementary stochastic simulation based on novel DNA stability data. A significant dependence of opening probability and waiting time between bubble events on the local DNA sequence is revealed and quantified for a biological sequence of the T7 bacteriophage. Quantitative agreement with data from fluorescence correlation spectroscopy (FCS) is demonstrated.Comment: 4 pages, 5 figures, to appear in Physical Review Letter

An operator-induced conformational change in the C-terminal domain of the λ repressor

4,4'-bis(1-anilino-8-naphthalenesulfonic acid (Bis-ANS), an environment-sensitive fluorescent probe for hydrophobic region of proteins, binds specifically to the C-terminal domain of λ repressor. The binding is characterized by positive cooperativity, the magnitude of which is dependent on protein concentration in the concentration range where dimeric repressor aggregates to a tetramer. In this range, positive cooperativity becomes more pronounced at higher protein concentrations. This suggests a preferential binding of Bis-ANS to the dimeric form of the repressor. Binding of single operator OR1 to the N-terminal domain of the repressor causes enhancement of fluorescence of the C-terminal domain bound Bis-ANS. The binding of single operator OR1 also leads to quenching of fluorescence of tryptophan residues, all of which are located in the hinge or the C-terminal domain. Thus two different fluorescent probes indicate an operator-induced conformational change which affects the C-terminal domain. The significance of this conformational change with respect to the function of λ repressor has been discussed

Bubble coalescence in breathing DNA: Two vicious walkers in opposite potentials

We investigate the coalescence of two DNA-bubbles initially located at weak segments and separated by a more stable barrier region in a designed construct of double-stranded DNA. The characteristic time for bubble coalescence and the corresponding distribution are derived, as well as the distribution of coalescence positions along the barrier. Below the melting temperature, we find a Kramers-type barrier crossing behaviour, while at high temperatures, the bubble corners perform drift-diffusion towards coalescence. The results are obtained by mapping the bubble dynamics on the problem of two vicious walkers in opposite potentials.Comment: 7 pages, 4 figure

Lamb Wave Modes in Coal-Tar-Coated Steel Plates

In order to study the feasibility of using ultrasonic Lamb wave modes for detection and sizing of corrosion-related flaws in buried steel pipelines, we have calculated Lamb wave modes and performed numerous experiments on steel plates coated on one side with coal-tar enamel. The purpose of this paper is to discuss these theoretical and experimental results

Theory of Adiabatic fluctuations : third-order noise

We consider the response of a dynamical system driven by external adiabatic fluctuations. Based on the `adiabatic following approximation' we have made a systematic separation of time-scales to carry out an expansion in $\alpha |\mu|^{-1}$, where $\alpha$ is the strength of fluctuations and $|\mu|$ is the damping rate. We show that probability distribution functions obey the differential equations of motion which contain third order terms (beyond the usual Fokker-Planck terms) leading to non-Gaussian noise. The problem of adiabatic fluctuations in velocity space which is the counterpart of Brownian motion for fast fluctuations, has been solved exactly. The characteristic function and the associated probability distribution function are shown to be of stable form. The linear dissipation leads to a steady state which is stable and the variances and higher moments are shown to be finite.Comment: Plain Latex, no figures, 28 pages; to appear in J. Phys.

Exotic phases in compact stars

We discuss how the co-existence of hyperons, antikaon condensate and color superconducting quark matter in neutron star interior influences the gross properties of compact stars such as, the equation of state and mass-radius relationship. We compare our results with the recent observations. We also discuss about superdense stars in the third family branch which may contain a pure color-flavor-locked (CFL) core.Comment: 6 pages, presented in "Strange Quarks in Matter" (SQM2003) conference, Atlantic Beach, NC, USA, March 12-17, 2003 and to be published in J. Phys.