Neutralizing antibodies in cats infected with feline immunodeficiency virus.

Sera from cats experimentally infected with five isolates of feline immunodeficiency virus (FIV) from various geographical regions and from FIV enzyme-linked immunosorbent assay-seropositive field cats from four European countries neutralized the Petaluma strain of FIV (FIV-P), originally isolated in California, at high titers. In addition, FIV-P and a European isolate proved equally susceptible to neutralization by all sera tested. Coupled with observations by Fevereiro et al. (M. Fevereiro, C. Roneker, A. Laufs, L. Tavares, and F. de Noronha, J. Gen. Virol. 72:617-622, 1991), these findings indicate that most if not all FIV strains circulating in Europe and the United States share important neutralization-inducing epitopes

Space mission design ontology : extraction of domain-specific entities and concepts similarity analysis

Expert Systems, computer programs able to capture human expertise and mimic experts’ reasoning, can support the design of future space missions by assimilating and facilitating access to accumulated knowledge. To organise these data, the virtual assistant needs to understand the concepts characterising space systems engineering. In other words, it needs an ontology of space systems. Unfortunately, there is currently no official European space systems ontology. Developing an ontology is a lengthy and tedious process, involving several human domain experts, and therefore prone to human error and subjectivity. Could the foundations of an ontology be instead semi-automatically extracted from unstructured data related to space systems engineering? This paper presents an implementation of the first layers of the Ontology Learning Layer Cake, an approach to semi-automatically generate an ontology. Candidate entities and synonyms are extracted from three corpora: a set of 56 feasibility reports provided by the European Space Agency, 40 books on space mission design publicly available and a collection of 273 Wikipedia pages. Lexica of relevant space systems entities are semi-automatically generated based on three different methods: a frequency analysis, a term frequency-inverse document frequency analysis, and a Weirdness Index filtering. The frequency-based lexicon of the combined corpora is then fed to a word embedding method, word2vec, to learn the context of each entity. With a cosine similarity analysis, concepts with similar contexts are matched

Generation of virus like particles for epizootic hemorrhagic disease virus

Epizootic hemorrhagic disease virus (EHDV) is a distinct species within the genus Orbivirus, within the family Reoviridae. The epizootic hemorrhagic disease virus genome comprises ten segments of linear, double stranded (ds) RNA, which are packaged within each virus particle. The EHDV virion has a three layered capsid-structure, generated by four major viral proteins: VP2 and VP5 (outer capsid layer); VP7 (intermediate, core-surface layer) and VP3 (innermost, sub-core layer). Although EHDV infects cattle sporadically, several outbreaks have recently occurred in this species in five Mediterranean countries, indicating a potential threat to the European cattle industry. EHDV is transmitted by biting midges of the genus Culicoides, which can travel long distances through wind-born movements (particularly over water), increasing the potential for viral spread in new areas/countries. Expression systems to generate self-assembled virus like particles (VLPs) by simultaneous expression of the major capsid-proteins, have been established for several viruses (including bluetongue virus). This study has developed expression systems for production of EHDV VLPs, for use as non-infectious antigens in both vaccinology and serology studies, avoiding the risk of genetic reassortment between vaccine and field strains and facilitating large scale antigen production. Genes encoding the four major-capsid proteins of a field strain of EHDV-6, were isolated and cloned into transfer vectors, to generate two recombinant baculoviruses. The expression of these viral genes was assessed in insect cells by monitoring the presence of specific viral mRNAs and by western blotting. Electron microscopy studies confirmed the formation and purification of assembled VLPs