Phytochemical screening, mineral analysis and evaluation of the diuretic effect of aqueous extract of Pericopsis laxiflora from male Sprague Dawley rats

Pericopsis laxiflora is among the medicinal plants used by the traditional practitioner in the treatment of many diseases. However, data on its diuretic power are lacking. Moreover, the effect of the preparation of traditional galenic formulations on the composition of phytocompounds and essential minerals and metals is unknown. The aim of this study was to show the effect of treatment on the phytocompounds   and minerals composition and to evaluate diuretic effect of Pericopsis laxiflora aqueous axtracts. The tube characterization reactions revealed flavonoids, sterols, terpenes, catechin tannins polyphenols and saponins in aqueous extracts. Gallic tannins  and quinones are absent, All compounds were generally present in same proportions in two aqueous extracts. Chemical analysis of all aqueous extracts by atomic absorption spectrometry (AAS) showed effect of model extraction on minerals. Copper (0.3116 mg / 100g), magnesium (260.7 mg /100 g), selenium (0.00904 mg /100 g), zinc (4.52 mg /100 g), manganese (75.029 mg /100g) are more concentrated in maceration than a   decoction. For decoction of same plant, the situation is reversed with sodium (58.6mg /100g), calcium (12670 mg/100g), iron (18.51mg/100g) and potassium (2781 mg /100g). For pharmacological investigation, 32 rats Sprague Dawley male weighing 385-433g are isolated each in metabolic cages from 6 pm to 8am  and treated with 100mg/kg of Pericopsis laxiflora aqueous extracts. From 8am to 2pm the animals received only tap water in metabolic cages. The volumes of water intake and urine were not significantly different in the two groups (treated and untreated).The diuretic effect was not observed with 100mg/kg (b/w) . In addition   polyphenols, flavonoids, saponins and minerals such as potassium, magnesium and selenium are indicators of the vasodilatory and cardioprotective functions of Pericopis laxiflora aqueous extracts. The prescription of these extracts in traditional medicine must take into account the toxicity of the plant associated with a calcium and manganese content. Keywords:  Pericopsis laxiflora, dueresis, decocted extract, macerated extract, minerals, Rats Sprague Dawele

Comparative study of the anticoagulant powers of the aqueous macerate, ethanolic macerate and aqueous decoction of the leaves of Uvaria chamae P. Beauv. (Annonaceae)

Data on the anticoagulant power of Uvaria chamae, a plant used in traditional medicine, remain unknown despite its many therapeutic and biological virtues. The present study aimed to compare the anticoagulant effects of aqueous macerate, ethanolic macerate and aqueous decoction of U. chamae leaves. The anticoagulant activity of macerated and decocted U. chamae was evaluated in vitro against endogenous and exogenous coagulation pathways in a pool of displaced normal plasmas. These two pathways are explored using two global and chronometric tests including Cephalin-Kaolin Time (TCK) and Prothrombin Time (TQ). The results obtained revealed that the incubation time of U. chamae macerates and decoctions with plasma significantly influences their anticoagulant power. This evaluation showed that these macerated and decocted products exerted anticoagulant power at concentrations dependent on these two coagulation pathways. This anticoagulant power at the level of the aqueous decocted was more significant (P ˂ 0.001) on these two coagulation pathways but it was more marked on the endogenous pathway than on the exogenous pathway. This anticoagulant power at the level of the aqueous decocted was more significant (P ˂ 0.001) on these two coagulation pathways but it was more marked on the endogenous pathway than on the exogenous pathway. This Ivorian plant could thus be considered as a natural source of anticoagulant agents for medicinal purposes. Keywords: Uvaria chamae, Anticoagulant, Aqueous macerate, Ethanolic macerate, Aqueous decocte