2 research outputs found
Analysis of polyacetylenes by HPLC in hairy root cultures of Lobelia inflata cultivated in bioreactor
Lobelia inflato L. contains pharmacologically important secondary
metabolites. The main alkaloid is lobeline which has a stimulatory
effect on the respiratory centre. Recently high quantity of
polyacetylenes was determined in the plant and hairy root cultures.
With the aim of increasing of the secondary metabolite production hairy
root cultures were produced (by direct infection of the sterile plants
with Agrobacterium rhizogenes strain R1601). A new HPLC method was
developed for the determination of lobetyolin and lobetyolinin, the
main polyacetylene compounds of L. inflata hairy roots. The analysis
was performed on Hypersil MOS column (250 x 4 mm I.D.). The mobile
phase was 15:85 (v/v) mixture of acetonitrile and water. Peaks were
identified by addition of standards and by DAD. The external
standardized method allowed the determination of lobetyolin and
lobetyolinin with good sensitivity and reliability. This method was
used to measure the polyacetylene contents of hairy roots grew
intensively in bioreactor. High contents of lobetyolin (3.6%) and
lobetyolinin (0.8-1.6%) were detected in the hairy roots because of
their great biosynthetic capacity
Essential oil constituents of intact plants and in vitro cultures of Tagetes patula L.
The occurrence of volatile compounds in flowers and roots of intact
plants and in vitro hairy root cultures of Tagetes patula L. was
investigated by GC and GC/MS. The flowers oil was dominated by mono-
and sesquiterpenes. The main component was the sesquiterpene
hydrocarbon beta-caryophyllene (50.2%). However, the oils of normal
roots and hairy root cultures were found to contain sulfur-containing
thiophene structures. The following four compounds were identified:
5-(3-buten-1-ynyl)-2,2'-bithiophene (BBT),
5-(3-penten-1-ynyl)-2,2'-bithiophene (PBT), 2,2':5',2"-terthiophene
(alpha-terthiophene) and 5-(4-acetoxy-1-butynyl)-2,2'-bithiophene
(BBTOAc). The main components were BBT (47.0%) and alpha-terthiophene
(19.8%) in the normal root oil and BBT (25.0%), alpha-terthiophene
(14.3%) and BBTOAc (13.7%) in hairy root oil, respectively