10 research outputs found
Comparative assessment of selected intraoral microorganisms – potential factors for peri-surgical management complications
In this research, a comparative analysis of results of investigations involving different human
populations, in terms of a relation between the oral cavity health and the species composition of mouth microbiota is
reported. The purpose of this analysis was to identify and assess microorganisms that could cause health complications
in patients with neoplasm requiring dental problem-related surgical management. The patients with the oral cancer
surgically treated and those without neoplasm were assessed for their oral health: status of teeth, gingiva, periodontium,
and occurrence of inflammatory processes. From each patient, microorganisms isolated of periodontium, dental plaque,
and dental pocket swabs were identified in wet and stained microscopic preparations; standard microbiology in vitro
techniques were also applied to determine the fungal and bacterial strains. The comparative analysis of results of direct
microscopic examinations and in vitro cultures assessment indicated significant differences in prevalences of fungi,
parasitic oral protozoans and bacteriae in particular patient’s groups. Yeast-like fungi belonging to Candida genus,
mostly of C. albicans group, were identified in 93.75% patients with the oral cancer, while in 25% of individuals
assessed without neoplasm. E. gingivalis amoebae were only found in 12.5% patients with the serious disease; no
trichomonads were detected in all patients analyzed. Among bacteria species, potentially pathogenic Enterobacteriaceae
were found in the patients with oral cancer. The pronounced shift in the microbiota species composition in the patients
who needed prolonged treatment due to oral cavity cancer, compared to other generally healthy persons has been
showed in this analysis
Evaluation of selected oral cavity microbiota – risk factors of management complications in patients with masticatory system disorders
The retrospective analysis of data on oral cavity clinical status in relation to microbiota species
composition is presented. The research regards patients of different age, with and without congenital malformation,
pretreatment assessed for occurrence of pathological changes in the masticatory system. Samples of the swabs collected
from each patient (from dental plaque, periodontium and dental pockets) were used for identification of oral protozoans
in wet slides and stained preparations; additionally, transmission electron microscope examination was performed. The
material was used for in vitro cultures to identify bacteria strains. Clinically, intensity of tissue deteriorations was higher
in patients with a congenital disease. Alive Trichomonas tenax and Entamoeba gingivalis, species with confirmed
pathogenic impact on oral cavity and neighboring structures, were detected with higher prevalence in older patients.
Enterococci, Staphylococcus aureus, various Enterobacteriaceae were more frequently detected in patients with somatic
and mental retardations; in mouths of those patients, Klebsiella pneumonia and Pseudomonas aeruginosa opportunistic
strains occurred. Masticatory system abnormalities favor colonization of oral cavity by exogenous species and
dissemination of infections, especially dangerous for patients with congenital diseases. Oral microbiota assessment and
preventive measures may be helpful to avoid subsequent peri-surgery complications
Evaluation of in vitro effect of selected contact lens solutions conjugated with nanoparticles in terms of preventive approach to public health risk generated by Acanthamoeba strains
Introduction. Various Acanthamoeba species are free-living organisms widely distributed in the human environment.
Amphizoic amoebae as facultative parasites may cause vision-threatening eye disease – Acanthamoeba keratitis, mostly
among contact lens wearers. As the number of cases is increasing, and applied therapy often unsuccessful, proper hygienic
measures and effective contact lenses disinfection are crucial for the prevention of this disease. Available contact lens solutions
are not fully effective against amphizoic amoebae; there is a need to enhance their disinfecting activity to prevent amoebic
infections. The use of developing nanotechnology methods already applied with success in the prevention, diagnostic and
therapy of other infectious diseases might be helpful regarding amoebic keratitis. This study assesses the in vitro effect of
selected contact lens solutions conjugated with nanoparticles against Acanthamoeba trophozoites.
Materials and method. Three selected contact lens solutions conjugated with silver and gold nanoparticles in concentration
of 0.25–2.5 ppm were used in vitro against the axenically cultured ATCC 30010 type Acanthamoeba castellanii strain. The
anti-amoebic efficacy was examined based on the oxido-reduction of AlamarBlue. The cytotoxicity tests based on the
measurement of lactate dehydrogenase (LDH) activity were performed using a fibroblast HS-5 cell line.
Results. Enhancement of the anti-amoebic activity of contact lens solutions conjugated with selected nanoparticles
expressed in the dose dependent amoebic growth inhibition with a low cytotoxicity profile was observed.
Conclusions. Results of the study showed that conjugation of selected contact lens solutions with silver nanoparticles
might be a promising approach to prevent Acanthamoeba keratitis among contact lens users