Age Determination from Biological Stains Using Messenger RNS Profiling Analysis

In the present work, we report the development of two reverse transcription-quantitative polymerase chain reacton (RT-qPCR) assays for the definitve identification of newborn individuals. The methodology is based upon gene expresiion profiling analysis in which specific age groups are identified by detecting the relative quantity of expression of appropriate mRNA species. RNA is isolated from dried bloodstains is analyzed using the two duplex real-time PCR reactions for two variant novel forms of newborn specific gamma hemoglobin

Success for pacific learners : the impact of tertiary education strategies

This project examined the impact of the 3 successive tertiary education strategies (2002-2007; 2007-2012 and 2010-2015) on how tertiary education institutions have developed their support for Pacific learners. Findings show that the Pasifika objectives within each of the 3 strategies are considered to be an important signal to Tertiary Education Institutions (TEIs) that success for Pasifika learners is a significant and continuing government priority and that the strategies are seen as an important enabler of change. These objectives have raised the priority for action to support Pasifika learners, unlocked resources for Pasifika initiatives and promoted the inclusion of Pasifika priorities on the strategic agendas of TEIs. It was noted however, this influence is only one of a number of internal and external, and local and national influences on enhancing success for Pasifika learners

mRNA Profiling: Body Fluid Identification Using Multiplex Real-Time PCR

We have developed real-time PCR triplexes that are composed of two body fluid-genes and one housekeeping gene (glyceraldhyde-3-phosphate dehydrogenase, or (GAPDH), and have been optimized for the detection of blood, saliva, semen, and menstrual blood as single or mixed stains

Age Determination from Biological Stains Using Messenger RNS Profiling Analysis. DIV

Reverse transcription-quantitative polymerase chain reaction (RT-qPCR) assays, systems, methods and kits for the age determination of an individual from bloodstains or samples of unknown origin. The methodology is based on gene expression profiling analysis in which novel human newborn fetal specific genes are identified by detecting the presence of appropriate messenger RNA species

Method for Determining the Origin of a Sample

A method for determining the nature of a sample is provided, wherein the presence or absence of at least one marker small non-coding RNA in the sample is detected. Suitable marker small non-coding RNAs for different samples such as blood, saliva, semen and vaginal secretions are provided. Also provided are suitable kits and methods for identifying marker small-non coding RNAs

Forensic trace DNA: A review

DNA analysis is frequently used to acquire information from biological material to aid enquiries associated with criminal offences, disaster victim identification and missing persons investigations. As the relevance and value of DNA profiling to forensic investigations has increased, so too has the desire to generate this information from smaller amounts of DNA. Trace DNA samples may be defined as any sample which falls below recommended thresholds at any stage of the analysis, from sample detection through to profile interpretation, and can not be defined by a precise picogram amount. Here we review aspects associated with the collection, DNA extraction, amplification, profiling and interpretation of trace DNA samples. Contamination and transfer issues are also briefly discussed within the context of trace DNA analysis. Whilst several methodological changes have facilitated profiling from trace samples in recent years it is also clear that many opportunities exist for further improvements

Local fusion graphs of finite groups

Let G be a group, with X a G-conjugacy class of involutions. The local fusion graph F(G,X) has X as its vertex set, with vertices x, y in X joined by an edge if, and only if, x is not equal to y and the order of the product xy is odd. In this thesis we study these, and other related graphs, for a variety of finite groups, paying particular attention to the cases where G is a finite group. We also present a computational algorithm regarding centralisers of involutions, which makes use of local fusion graphs.EThOS - Electronic Theses Online ServiceGBUnited Kingdo

Novel thermostable Y-family polymerases: applications for the PCR amplification of damaged or ancient DNAs

For many years, Taq polymerase has served as the stalwart enzyme in the PCR amplification of DNA. However, a major limitation of Taq is its inability to amplify damaged DNA, thereby restricting its usefulness in forensic applications. In contrast, Y-family DNA polymerases, such as Dpo4 from Sulfolobus solfataricus, can traverse a wide variety of DNA lesions. Here, we report the identification and characterization of five novel thermostable Dpo4-like enzymes from Acidianus infernus, Sulfolobus shibatae, Sulfolobus tengchongensis, Stygiolobus azoricus and Sulfurisphaera ohwakuensis, as well as two recombinant chimeras that have enhanced enzymatic properties compared with the naturally occurring polymerases. The Dpo4-like polymerases are moderately processive, can substitute for Taq in PCR and can bypass DNA lesions that normally block Taq. Such properties make the Dpo4-like enzymes ideally suited for the PCR amplification of damaged DNA samples. Indeed, by using a blend of Taq and Dpo4-like enzymes, we obtained a PCR amplicon from ultraviolet-irradiated DNA that was largely unamplifyable with Taq alone. The inclusion of thermostable Dpo4-like polymerases in PCRs, therefore, augments the recovery and analysis of lesion-containing DNA samples, such as those commonly found in forensic or ancient DNA molecular applications

Neurological impairment in nephropathic cystinosis: motor coordination deficits

Nephropathic cystinosis is a rare genetic metabolic disorder that results in accumulation of the amino acid cystine in lysosomes due to lack of a cystine-specific transporter protein. Cystine accumulates in cells throughout the body and causes progressive damage to multiple organs, including the brain. Neuromotor deficits have been qualitatively described in individuals with cystinosis. This study quantitatively examined fine-motor coordination in individuals with cystinosis. Brain magnetic resonance imaging (MRI) scans were also performed to determine whether structural changes were associated with motor deficits. Participants were 52 children and adolescents with infantile nephropathic cystinosis and 49 controls, ages 2–17 years, divided into preacademic and school-age groups. Results indicated that both the preacademic and school-age cystinosis groups performed significantly more poorly than their matched control groups on the Motor Coordination Test. Further, the level of performance was not significantly different between the preacademic and school-age groups. There were no significant differences in motor coordination scores based on MRI findings. This is the first study to document a persistent, nonprogressive, fine-motor coordination deficit in children and adolescents with cystinosis. The fact that these difficulties are present in the preschool years lends further support to the theory that cystinosis adversely affects neurological functioning early in development. The absence of a relationship between brain structural changes and motor function suggests that an alternative cause for motor dysfunction must be at work in this disorder

Exposure-age constraints on the extent, timing and rate of retreat of the last Irish Sea ice stream

We report 23 cosmogenic isotope exposure ages (10Be and 36Cl) relating to the maximum extent and deglaciation chronology of the Irish Sea Ice Stream (ISIS), which drained the SW sector of the last British-Irish Ice Sheet. These show that the ISIS failed to reach the Preseli Hills of North Pembrokeshire yet extended southwards to impinge on northern Isles of Scilly (50°N) during the last glacial maximum. Four samples from western Anglesey demonstrate deglaciation of the southern Irish Sea Basin by c. 20-18 ka, and two from the Llŷn Peninsula in northwest Wales, if valid, suggest deglaciation by c. 23-22 ka followed by gradual oscillatory northwards retreat of the ice margin for over 3000 years. An alternative interpretation of our data suggests that ice reached Scilly as late as 22-21 ka then retreated 450 km northwards within the following three millennia, possibly in response to sea level rise and/or intrinsic reorganisation within the last British-Irish Ice Sheet. Samples from upland source areas of the ISIS in NW England and SW Scotland produced exposure ages ≤14.3 ka, suggesting possible persistence of ice in such areas into the Lateglacial Interstade of 14.7-12.9 ka