Chronic ouabain treatment increases the contribution of nitric oxide to endothelium-dependent relaxation

The aim of this study was to analyze the contribution of nitric oxide, prostacyclin and endothelium-dependent hyperpolarizing factor to endothelium-dependent vasodilation induced by acetylcholine in rat aorta from control and ouabain-induced hypertensive rats. Preincubation with the nitric oxide synthase inhibitor N-omega-nitro-L-arginine methyl esther (L-NAME) inhibited the vasodilator response to acetylcholine in segments from both groups but to a greater extent in segments from ouabain-treated rats. Basal and acetylcholine-induced nitric oxide release were higher in segments from ouabain-treated rats. Preincubation with the prostacyclin synthesis Inhibitor tranylcypromine or with the cyclooxygenase inhibitor indomethacin inhibited the vasodilator response to acetylcholine in aortic segments front both groups. The Ca(2+)-dependent potassium channel blocker charybdotoxin inhibited the vasodilator response to acetylcholine only In segments from control rats. These results indicate that hypertension induced by chronic ouabain treatment is accompanied by increased endothelial nitric oxide participation and impaired endothelium-dependent hyperpolarizing factor contribution In acetylcholine-induced relaxation. These effects might explain the lack of effect of ouabain treatment oil acetylcholine responses in rat aorta.Instituto de Salud Carlos IIIInstituto de Salud Carlos III[PI051767]Instituto de Salud Carlos III[RD06/0014/0011]Instituto de Salud Carlos IIIMinisterio de Educacion y Ciencia[SAF2006-02376]Ministerio de Educacion y CienciaMinisterio de Educacion y Ciencia[SAF2006-07888]Ministerio de Educacion y CienciaFundacion Medica Mutua MadrilenaFundacion Medica Mutua Madrilena[MMA-06]Branco de Santander Central HispanoBranco de Santander Central Hispan