6 research outputs found
Significance of survivin immunoreactivity and morphometric analysis of HPV-induced cervical dysplasia
Genomic integration of high-risk human papilloma virus in the nucleus of
cervical epithelial mucosal cells leads to epithelial dysplasia. The aim of
this study was to determine the relevance of correlation between epithelial
survivin expression and the degree of human papilloma virus (HPV)-induced
cervical epithelial dysplasia, and to establish the significance of
morphometric analysis of the nuclear area in the assessment of the degree of
cervical dysplasia. This retrospective study included 99 women with primary,
previously untreated lesions, and colposcopic findings indicating dysplasia,
in whom a cytological test by Papanicolaou method was interpreted according
to the Bethesda criteria as lowgrade squamous intraepithelial lesion (LSIL),
high-grade squamous intraepithelial lesion (HSIL), and atypical squamous
cells of undetermined significance (ASCUS). We performed human papilloma
virus (HPV) typing by PCR for evidence of viruse types 16, 18, 31, 33. After
biopsy of the cervical mucosa, we performed hematoxylin-eosin (H-E) and
Periodic Acid Schiff (PAS) staining, and immunohistochemical and morphometric
analysis of tissue samples. The control group consisted of 12 women without
dysplasia and without a verified infection of cervical high-risk HPV. A high
statistical correlation between the degree of dysplasia and expression of
survivin was found in patients with different types of cervical dysplasia (p
= 0.003). We observed a high statistical difference between the area of
nuclei at different degrees of cervical dysplasias (p = 0.000). The
high-grade cervical dysplasia had a more than 2-fold higher level of ranking
in comparison to low-grade dysplasia, and a more than 10-fold higher ranking
than the control group without cervical dysplasia
Eozinofilna upala u alergijskom rinitisu i nosnoj polipozi
On histopathological examination, nasal polyps and nasal mucosa in allergic rhinitis show different forms of pseudostratified respiratory epithelium, whereas the dominant characteristic of lamina propria is an eosinophilic infiltration. The aim of this study was to compare interleukin (IL)-5 and eosinophilic cationic protein (ECP) levels in the nasal fluid of 42 patients: 12 with allergic rhinitis and nasal septal deviation, 17 non-atopic patients with nasal polyposis, and 13 atopic nasal polyp patients were enrolled in this cross-sectional study. Nasal secretion samples were collected a few days before surgery. The levels of IL-5 were measured using fl ow cytometry and the ECP using a commercial ELISA kit. In addition, we counted eosinophils in hematoxylin-and-eosin-stained sections of all nasal polyp and all nasal mucosa samples taken from the inferior nasal turbinates during septoplasty. A significantly higher concentration of IL-5 was found in the nasal fluid of atopic patients with nasal polyposis than in non-atopic nasal polyp patients (p=0.025) and patients with allergic rhinitis (p=0.05). ECP was higher in atopic nasal polyp patients than in patients with allergic rhinitis (p<0.0001) and than in non-atopic nasal polyp patients (p<0.0001). Polyp eosinophils were higher in atopicā than in non-atopic patients (p<0.0001) and higher than in the mucosa of patients with allergic rhinitis (p<0.0001). These however had signifi cantly more mucosal eosinophils than was found in the polyps of non-atopic patientsā (p=0.025). ECP levels in nasal fl uid and eosinophil counts in tissue specimens correlated well in all three groups of patients. Our study has shown that atopic nasal polyp patients have a higher level of eosinophilic inflammation than non-atopic patients with nasal polyps and patients with allergic rhinitis.Na histopatoloÅ”kim pregledima nosni polipi, kao i nosna sluznica u alergijskom rinitisu pokazuju razliÄite oblike pseudoslojevitog respiracijskog epitela, dok je osnovna karakteristika lamine proprije infiltracija eozinofilima. Cilj ove studije bio je usporediti koncentracije interleukina 5 (IL-5) i eozinofilnoga kationskog proteina (ECP) u nosnome sekretu pacijenata s alergijskim rinitisom, neatopiÄnih i atopiÄnih pacijenata s nosnom polipozom, kao i usporediti broj eozinofi la u tkivu nosnih polipa/nosne sluznice ovih pacijenata. Äetrdeset dvoje (n=42) pacijenata, 12-ero s alergijskim rinitisom i devijacijom nosnog septuma, 17-ero neatopiÄnih pacijenata s nosnom polipozom i 13-ero atopiÄnih pacijenata s nosnom polipozom bilo je ukljuÄeno u ovu presjeÄnu studiju. Uzorci nosnog sekreta bili su skupljeni iz nosnih Å”upljina svih ispitanika nekoliko dana prije kirurÅ”kog lijeÄenja. Koncentracije IL-5 mjerene su metodom protoÄne citometrije, dok su koncentracije ECP mjerene komercijalnim ELISA-kitom. Eozinofi li su brojeni u svim uzorcima tkiva nosnih polipa, kao i u svim uzorcima tkiva sluznice uzetih s donje nosne Å”koljke tijekom septoplastike. ZnaÄajno viÅ”e koncentracije IL-5 izmjerene su u nosnom sekretu atopiÄnih pacijenata s nosnom polipozom u usporedbi s neatopiÄnim pacijentima s nosnom polipozom (p=0,025) i pacijentima s alergijskim rinitisom (p=0,05). NaÅ”i su rezultati pokazali viÅ”e koncentracije ECP u atopiÄnih pacijenata s nosnom polipozom nego u pacijenata s alergijskim rinitisom (p<0,0001) i u usporedbi s nealergiÄnim pacijentima s nosnom polipozom (p<0,0001). VeÄi broj eozinofi la izbrojen je u tkivu polipa atopiÄnih pacijenata nego u tkivu polipa neatopiÄnih pacijenata (p<0,0001), kao i u sluznici pacijenata s alergijskim rinitisom (p<0,0001). U nosnoj sluznici pacijenata s alergijskim rinitisom naÅ”li smo znaÄajno veÄi broj eozinofila nego u polipima neatopiÄnih pacijenata s nosnim polipima (p=0,025). KonaÄno, naÅ”li smo pozitivnu korelaciju izmeÄu nivoa ECP u nosnom sekretu i broja eozinofila u uzorcima tkiva u sve tri skupine ispitanika. ZakljuÄili smo da atopiÄni pacijenti s nosnom polipozom imaju viÅ”i stupanj eozinofilne upale u usporedbi s neatopiÄnim pacijentima s nosnim polipima i s pacijentima s alergijskim rinitisom
Citizenship and Social Welfare in Croatia: Clientelism and the Limits of āEuropeanisationā
The influence of demogeographic development on spatial contents and models of primary education in Istria
This work explores the influence of demographic processes, especially
birth rateās movements, on the changes in the features and
organisation of primary education in Istria between years 2001
and 2017. The analyses were implemented on the level of municipalities
and cities, and greater spatial units of Istria ā coastal area,
the inland and the city of Pula. We analysed the changes in population
and student contingent, fluctuations of birth rate, net
migration and the changes in primary education (number of students
and classes, number of teachers and schools). The results
have pointed out to the existence of a positive connection
between birth rateās change and number of students in primary
schools, but also that birth rate has a postponed effect on the
size of the student contingent. Spatial disparities between the
Istriaās inland and coast are determined also in the features of primary
education that generally adapts to demographic processes
more slowl