Aislamiento de RNA mensajeros que codifican antígenos recombinantes desplegados diferencialmente en estados virulento y no virulento por Entamoeba Histolytica

Tesis ( Doctorado en Ciencias con Especialidad en Biotecnología) UANLUANLhttp://www.uanl.mx

Diseño de un curso enfocado al desarrollo humano integral para estudiantes del nivel superior de la Universidad Autónoma de Nuevo León : propuesta didáctica

[Tesis] ( Maestría en la Enseñanza de las Ciencias con Especialidad en Biología) U.A.N.L.UANLhttp://www.uanl.mx

Analysis of mortality during the winter seasons in Mexico from 2000 to 2012

To analyze mortality from all causes in Mexico during the winter months. Methods: Data was extracted and tabulated on monthly all-cause mortality in the general population from 2000 to 2012 from the INEGI database. Coeficients of seasonal variation in mortality were calculated. Results: In Mexico there was an increase of 14 % in mortality in the general population during winter. The more susceptible age groups were older people and children, with increases of 18% and 13% respectively. The months with low levels of mortality were April, October and September for children, adolescents and older people respectively. Conclusions: Important increases in winter mortality occur in Mexico

Stereochemical preference toward oncotarget: Design, synthesis and <i>in vitro</i> anticancer evaluation of diastereomeric &#x3B2;-lactams

ABSTRACT Purpose: In the battle against cancer discovery of new and novel chemotherapeutic agent demands extreme obligation. Development of anticancer compounds with higher potency and reduced side-effects is timely and challenging. Experimental Design: A small series of fourteen diastereomeric β-lactams (seven pairs) were synthesized through multi-step process exploring [2+2] ketene-imine cycloaddition as the key step. Comparative stereochemical preferences were studied through computational docking and validated by in vitro evaluation. β-tubulin was considered as possible molecular target and in vitro anticancer evaluation was conducted against SiHa, B16F10, K562 and Chang cell lines. Caspase-3 activation assay and hematoxylin/eosin staining of the cells were also accomplished. Results: Better docking scores of the cis- over the trans-β-lactams indicated favorable β-lactam—β-tubulin interactions in cis-geometry. In vitro (IC50) evaluation confirmed better anticancer activity of the cis-diastereoisomers. Apoptosis-induced cell death was supported by caspase-3 activation study. A cis-β-lactam [(±)-Cis3-amino-1-phenyl-4-(p-tolyl) azetidin-2-one, 6C] was found to be more active (in vitro) than the marketed natural drug colchicine against SiHa and B16F10 (six times higher potency) cell lines. Reduced toxicity (compared to colchicine) in Chang cells confirmed better site-selectivity (accordingly less side-effects) of 6C than colchicine. Aside from 6C, most of the reported molecules demonstrated good to strong in vitro anticancer activity against SiHa and B16F10 cancer cell lines. Conclusions: Stereochemical preferences of the cis-β-lactams over their transcounterparts, toward the molecular target β-tubulin, was confirmed by docking studies and in vitro anticancer evaluation. Apoptosis was identified as the cause of cell death. The lead 6C exhibited higher potency and selectivity than the marketed drug colchicine both in silico as well as in vitr