Trypsin digest of cancer cells surface stimulates anti-tumor immune response better than cancer cells themselves

Antigens expressed on the surface of cancer cells are accessible targets for both humoral and cell-mediated immune responses, and are therefore potential candidates for vaccine development. Treating surface of live human breast adenocarcinoma cells (MCF-7) with trypsin yields a digest that contains 0.7% of total cell protein. Despite this difference, the trypsin digest stimulates in cytotoxicity assays anti-tumor response which kills 10-40% more cancer cells than those stimulated with cells themselves. From these results, we concluded that trypsin digest obtained from live cancer cells contains the essential antigens to induce an immune-mediated anti-tumor effect, and therefore, is candidate for anti-tumor vaccine development

Cell Proteomic Footprint

The authentication of mammalian cell cultures and their subpopulations are of tremendous demand in biotechnology and cell therapy. However, current techniques are either not efficient or can be very complex and expensive. Here we report a simple and straightforward approach for authentication of biological cells and their subpopulations with high speed, high throughput, low sample cost, and high sensitivity. We discovered that cell cultures treated with protease at soft, “non-killing” conditions release fragments of cell surface proteins, which composition is a strong characteristic of the cells. Mass spectrometric analysis of the released fragments allows a direct comparison of the produced mass spectrum with the mass spectrum of known cells. As an example, we applied this technique to verify subpopulations of human fibroblasts which have different origins and exhibit different medical characteristics

Granulomatous Interstitial Nephritis in Children Resulting from Wegener’s Granulomatosis, Crohn’s Disease, or Sarcoidosis

Granulomatous interstitial nephritis (GIN) is a rare type of kidney disease, the precise etiology of which is obscure, but is most commonly seen following drug therapy and infection. The main infections seen in the onset of this pathology, especially in immunocompromised patients, include mycobacteria and fungi. Granulomatous interstitial nephritis can be a manifestation of systemic diseases, such as Wegener’s granulomatosis, Crohn’s disease, or sarcoidosis. We present our experience with GIN diagnosis and management

Structure-Aware Shape Synthesis

We propose a new procedure to guide training of a data-driven shape generative model using a structure-aware loss function. Complex 3D shapes often can be summarized using a coarsely defined structure which is consistent and robust across variety of observations. However, existing synthesis techniques do not account for structure during training, and thus often generate implausible and structurally unrealistic shapes. During training, we enforce structural constraints in order to enforce consistency and structure across the entire manifold. We propose a novel methodology for training 3D generative models that incorporates structural information into an end-to-end training pipeline.Comment: Accepted to 3DV 201

Russisches Arbeitsrecht: Große Fortschritte bei der Formalisierung

Mit der Neuregelung 2002 und den gerade erfolgten Nachbesserungen hat Russland nun ein umfassend reformiertes Arbeitsrecht, das nun auch in der Praxis zunehmend durchgesetzt wird. Die in den 1990er Jahren üblichen weitgehend unregulierten Beschäftigungsverhältnisse und Schwarzgeldzahlungen verlieren drastisch an Bedeutung. Das Arbeitsrecht ist in vielen seiner Bestimmungen sehr arbeitnehmerfreundlich. Der Mangel an qualifizierten Fachkräften führt zumindest in den großen Metropolen auch zu steigenden Löhnen

Norwegian Experience as a Promising Measure for the Russian Energy System Development

At the moment, Arctic is in the interest of many countries, and not only near the Arctic. A high degree of the region promising in the context of undiscovered oil and gas resources is the explanation for it. Russia possessing the most significant share of these reserves has a fairly low rate of investigation of the Arctic shelf. Arctic hydrocarbon resources occupy the important strategic place in the development of the fuel-energy complex of Russia, ensuring its energy security. Hence, the goal of this research is to identify effective model of the offshore fields development in the Arctic zone of the Russian Federation. The Norwegian model of formation of consortia for the development of the offshore fields is analyzed and it is examined in the context of the Russian Arctic. The effectiveness of the introduction of this model in Russia is proved. Keywords: consortium, energy system, Arctic shelf, Arctic zone of the Russian Federation. JEL Classifications: Q43, Q48, R1