1 research outputs found
Full-aperture extended-depth oblique plane microscopy through dynamic remote focusing
Oblique plane microscopy is a method enabling light-sheet fluorescence
imaging through a single microscope objective lens by focusing on a tilted
plane within the sample. To focus the fluorescence emitted by the oblique plane
on a camera, the light is imaged through a pair of remote objective lenses,
facing each other at an angle. The aperture mismatch resulting from this
configuration limits the effective numerical aperture of the system, reducing
image resolution and signal intensity.
This manuscript introduces an alternative method to capture the oblique plane
on the camera. Instead of relying on angled objective lenses, an electrically
tunable lens is employed. This lens adjusts the focal plane of the microscope
synchronously with the rolling shutter of a scientific CMOS camera. In this
configuration the entire aperture of the objective is effectively employed,
increasing the resolution of the system. Moreover, a variety of objective
lenses can be employed, enabling the acquisition of wider axial fields of view
compared to conventional oblique plane microscopy