Pulmonary dysfunction-an overt leprosy sequel: study done in a rehabilitation centre

Background:In India, a total of 1.35 Lakh new cases were detected during the year 2012-13, which gives annual new case detection rate (ANCDR) of 10.78 per 100,000 populations. The proportion of multibacillary cases is 49.92 %. New cases continue to occur in almost all the endemic countries indicating that high burden pocket can exist against a low burden back ground. Leprosy is a mono neuritis multiplex complex of immunological origin that results in autonomic, sensory and motor neuropathy. This impairs pulmonary chemo sensitivity because of the block of vagus and sympathetic plexus in leprosy patients. Even after the treatment, a predisposition to nerve damage continues, as the clearance of bacterial antigens is extremely slow. The study aims at assessing the respiratory function in treated leprosy patients.Methods: 23 treated leprosy patients were selected after written and informed consent according to the inclusion and exclusion criteria. After obtaining the clearance from Institutional ethical committee, pulmonary function test was performed and the lung parameters were measured using computerized spirometry.Results: The statistical analysis revealed significant decrease in FVC%, FEV1%, FEV1/FVC% in lepromatous patients compared with tuberculoid patients. The restrictive pattern predominates in both lepromatous and tuberculoid patients.Conclusions: The study concluded that there is a definite impairment of lung functions in the patients treated for leprosy, causing spontaneous respiratory arrest and unexplained sudden deaths. Since the results have statistical significance, the study warrants early detection of respiratory abnormalities in leprosy patients who don’t have any clinically detectable symptoms.

Pulmonary dysfunction-an overt leprosy sequel: study done in a rehabilitation centre

Background: In India, a total of 1.35 Lakh new cases were detected during the year 2012-13, which gives annual new case detection rate (ANCDR) of 10.78 per 100,000 populations. The proportion of multibacillary cases is 49.92 %. New cases continue to occur in almost all the endemic countries indicating that high burden pocket can exist against a low burden back ground. Leprosy is a mono neuritis multiplex complex of immunological origin that results in autonomic, sensory and motor neuropathy. This impairs pulmonary chemo sensitivity because of the block of vagus and sympathetic plexus in leprosy patients. Even after the treatment, a predisposition to nerve damage continues, as the clearance of bacterial antigens is extremely slow. The study aims at assessing the respiratory function in treated leprosy patients. Methods: 23 treated leprosy patients were selected after written and informed consent according to the inclusion and exclusion criteria. After obtaining the clearance from Institutional ethical committee, pulmonary function test was performed and the lung parameters were measured using computerized spirometry. Results: The statistical analysis revealed significant decrease in FVC%, FEV1%, FEV1/FVC% in lepromatous patients compared with tuberculoid patients. The restrictive pattern predominates in both lepromatous and tuberculoid patients. Conclusions: The study concluded that there is a definite impairment of lung functions in the patients treated for leprosy, causing spontaneous respiratory arrest and unexplained sudden deaths. Since the results have statistical significance, the study warrants early detection of respiratory abnormalities in leprosy patients who don't have any clinically detectable symptoms. [Int J Res Med Sci 2016; 4(7.000): 2843-2849

Cytological Method of Barr Body Expression in Dental Pulp Tissue at Varying Temperature

Introduction: Natural disasters in large population need major attention in person identification. It can be performed by means of simple techniques that are easier to use. Aim: To identify the Barr body in female dental pulp by cytological method, after exposing at varying temperature. Objective: The expression of Barr bodies was compared among different age groups, various temperature, and two types of stain. Materials and Methods: Teeth obtained from 60 female individuals were divided into two groups based on their age. In each group, 30 teeth were exposed at three different temperatures (200°C, 300°C, and 400°C), 10 teeth in each temperature. The pulp tissue obtained from teeth was centrifuged and stained with hematoxylin and eosin (H&E) and Papanicolaou stain (PAP). Presence of visible sex chromatin in the nuclear periphery was considered positive for Barr chromatin test. The results obtained were analyzed by both descriptive analysis and independent t-test. Result: The peripherally condensed Barr body in hyperchromatic nuclear outline was clearly visible in younger individuals at 200°C and is more accurately expressed in H&E stain. Conclusion: In our study, based on the results obtained from the smear of pulp tissue, expression of Barr bodies’ decreases as age and temperature increases. Further study with increase in sample size is mandatory to confirm the above conclusion