6 research outputs found
Role of quercetin and arginine in ameliorating nano zinc oxide-induced nephrotoxicity in rats
BACKGROUND: Nanoparticles are small-scale substances (<100 nm) with unique properties. Therefore, nanoparticles pose complex health risk implications. The objective of this study was to detect whether treatment with quercetin (Qur) and/or arginine (Arg) ameliorated nephrotoxicity induced by two different doses of nano zinc oxide (n-ZnO) particles. METHOD: ZnO nanoparticles were administered orally in two doses (either 600 mg or 1 g/Kg body weight/day for 5 conscutive days) to Wister albino rats. In order to detect the protective effects of the studied antioxidants against n-ZnO induced nepherotoxicity, different biochemical parameters were investigated. Moreover, histopathological examination of kidney tissue was performed. RESULTS: Nano zinc oxide-induced nephrotoxicity was confirmed by the elevation in serum inflammatory markers including: tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6); and C-reactive protein (CRP). Moreover, immunoglobulin (IGg), vascular endothelium growth factor (VEGF), and nitric oxide (NO) were significantly increased in rat serum. Serum urea and creatinine levels were also significantly increased in rats intoxicated with n-ZnO particles compared with the control group. Additionally, a significant decrease in the non-enzymatic antioxidant reduced glutathione (GSH) was shown in kidney tissues and serum glucose levels were increased. These biochemical findings were supported by a histopathological examination of kidney tissues, which showed that in the animals that received a high dose of n-ZnO, numerous kidney glomeruli underwent atrophy and fragmentation. Moreover, the renal tubules showed epithelial desquamation, degeneration and necrosis. Some renal tubules showed casts in their lumina. Severe congestion was also observed in renal interstitium. These effects were dose dependent. Cotreatment of rats with Qur and/or Arg along with n-ZnO significantly improved most of the deviated tested parameters. CONCLUSIONS: The data show that Qur has a beneficial effect against n-ZnO oxidative stress and related vascular complications. Also, its combination with Arg proved to be even more effective in ameliorating nano zinc oxide nephrotoxicity
Role of quercetin and arginine in ameliorating nano zinc oxide-induced nephrotoxicity in rats
Abstract Background Nanoparticles are small-scale substances ( Method ZnO nanoparticles were administered orally in two doses (either 600 mg or 1 g/Kg body weight/day for 5 conscutive days) to Wister albino rats. In order to detect the protective effects of the studied antioxidants against n-ZnO induced nepherotoxicity, different biochemical parameters were investigated. Moreover, histopathological examination of kidney tissue was performed. Results Nano zinc oxide-induced nephrotoxicity was confirmed by the elevation in serum inflammatory markers including: tumor necrosis factor alpha (TNF-α), interleukin-6 (IL-6); and C-reactive protein (CRP). Moreover, immunoglobulin (IGg), vascular endothelium growth factor (VEGF), and nitric oxide (NO) were significantly increased in rat serum. Serum urea and creatinine levels were also significantly increased in rats intoxicated with n-ZnO particles compared with the control group. Additionally, a significant decrease in the non-enzymatic antioxidant reduced glutathione (GSH) was shown in kidney tissues and serum glucose levels were increased. These biochemical findings were supported by a histopathological examination of kidney tissues, which showed that in the animals that received a high dose of n-ZnO, numerous kidney glomeruli underwent atrophy and fragmentation. Moreover, the renal tubules showed epithelial desquamation, degeneration and necrosis. Some renal tubules showed casts in their lumina. Severe congestion was also observed in renal interstitium. These effects were dose dependent. Cotreatment of rats with Qur and/or Arg along with n-ZnO significantly improved most of the deviated tested parameters. Conclusions The data show that Qur has a beneficial effect against n-ZnO oxidative stress and related vascular complications. Also, its combination with Arg proved to be even more effective in ameliorating nano zinc oxide nephrotoxicity.</p
Lisinopril has a cardio-protective effect on experimental acute autoimmune myocarditis in rats
The present study investigated the effect of
lisinopril on experimental autoimmune myocarditis
(EAM) in rats, a histologically similar model to human
acute myocarditis.
Animals and methods. Twenty four, six week-old
male Wistar rats were randomly allocated into 4 groups
of 6 rats each. Group I received no treatment. Group II
received lisinopril at a dose of 15 mg/kg/day suspended
in 1 ml of 2% gum acacia daily, from day 1 to day 21. To
induce myocarditis, animals of groups III and IV were
injected by 1 mg of porcine cardiac myosin on days 1
and 8. In addition, animals of group IV received
lisinopril in gum acacia daily, from day 1 to day 21. All
rats were sacrificed on day 21. Serum levels of creatine
phosphokinase, troponin-T, tumor necrosis factor-α and
interleukin-6 were estimated. Hearts were processed for
histopathological, as well as immunohistochemical study
for thioredoxin (TRX) immunoreactivity.
Results. The wall of hearts from rats of myocarditislisinopril group showed mild focal myocarditis and a
significant decrease of the mean percentage of pyknotic
nuclei in cardiomyocytes, coincident with a significant
decrease in serum biomarkers levels and TRX
immunoreactivity, compared to myocarditis group.
Conclusion. The present study suggested a cardioprotective effect of lisinopril on acute EAM in rats,
probably through a mechanism related to its suppressive
effect on angiotensin II formation