Guiding stem cell tenogenesis by modulation of growth factor signaling and cell-scale biophysical cues in bioengineered constructs

Tendon injuries and tendinopathies are increasingly prevalent health problems currently lacking effective treatments. Tissue engineering offers promising strategies to boost the low innate regenerative ability of tendons. Within this context, the simultaneous leveraging of both physical and biochemical cues by engineered scaffolding systems can be explored to promote a stronger tenogenic response from stem cells. Here, molecularly imprinted polymeric nanoparticles (MINPs) against transforming growth factor (TGF)-β3 are combined with bioinspired anisotropic hydrogels to produce tenogenesis-inductive constructs. MINPs are first solid phase-imprinted against a TGF-β3 epitope, achieving an affinity comparable to monoclonal antibodies. MINPs and magnetically-responsive microfibers are then encapsulated together with adipose-derived stem cells within gelatin-based hydrogels, applying a magnetostatic field during gelation to align the microfibers. The created anisotropic microstructure guides cell growth and elongation unidirectionally, while MINPs act as artificial receptors for TGF-β3, potentiating its paracrine action in the cellular microenvironment. The combination of both stimuli proves effective at increasing TGF-β signaling, which promotes the expression of tendon-associated genes and corresponding protein synthesis, suggesting that microstructural cues and biomolecule sequestration act in tandem to direct cell fate commitment. Overall, this system recapitulates several elements of tendon development, constituting a promising strategy for the regeneration of this tissue

Effect of abscisic acid applications on cold tolerance in chickpea (Cicer arietinum L.)

A series of field experiments were undertaken at three locations in Khyber PukhtunKhwa (KPK) Province, Pakistan to assess the effects of low temperatures and phytohormone applications on chickpea (Cicer arietinum L.) growth and yield. These trials showed that ABA application (10−4 M) to 40-day-old plants (before the first seasonal frost) offset low temperature-induced growth and yield depression at harvest (200-day-old plants) by up to 17%. These yield improvements were mainly due to an increase in the number of seeds pod−1. Growth room experiments were carried out under controlled environmental conditions to establish how foliar application of 10−4 M ABA to 40-day-old plants might improve seed production at harvest. The foliar application of 10−4 M ABA had no detectable effect on endogenous shoot or root ABA levels four-days after spraying or on biomass when plants were maintained in warm conditions. When exposed to night temperatures of −2 °C, however, the endogenous ABA levels increased dramatically in both control and ABA-treated plants, but this rise was more rapid after ABA application (p < 0.01); after 14 days, these plants had gained significantly more biomass than the unsprayed controls (p < 0.05). No evidence was found to suggest ABA affected the osmotic or water balance of plants, but parallel experiments have shown ABA reduced low temperature-induced cell damage. Analysis of the proteome of the shoot tissues of ABA treated and untreated plants by 2-Dimensional Gel Electrophoresis identified several proteins that are induced by low temperatures and/or by ABA application in chickpea and which may be involved in conferring coldtolerance. Attempts were made to establish the identity of these proteins using mass spectrometry but in all cases the results were ambiguous; a more complete protein data base for legumes is required before the function of these proteins can be inferred

Stateflow-based energy management strategy for hybrid energy system to mitigate load shedding

This study investigates the potential application of Stateflow (SF) to design an energy management strategy (EMS) for a renewable-based hybrid energy system (HES). The SF is an extended finite state machine; it provides a platform to design, model, and execute complex event-driven systems using an interactive graphical environment. The HES comprises photovoltaics (PV), energy storage units (ESU) and a diesel generator (Gen), integrated with the power grid that experiences a regular load shedding condition (scheduled power outages). The EMS optimizes the energy production and utilization during both modes of HES operation, i.e., grid-connected mode and the islanded mode. For islanded operation mode, a resilient power delivery is ensured when the system is subjected to intermittent renewable supply and grid vulnerability. The contributions of this paper are twofold: first is to propose an integrated framework of HES to address the problem of load shedding, and second is to design and implement a resilient EMS in the SF environment. The validation of the proposed EMS demonstrates its feasibility to serve the load for various operating scenarios. The latter include operations under seasonal variation, abnormal weather conditions, and different load shedding patterns. The simulation results reveal that the proposed EMS not only ensures uninterrupted power supply during load shedding but also reduces grid burden by maximizing the use of PV energy. In addition, the SF-based adopted methodology is envisaged to be a useful alternative to the popular design method using the conventional software tools, particularly for event-driven systems