A gas-chromatographie study of hydrocarbon formation in natural samples containing sulfate-reducing bacteria

A procedure is described for the gas-chromatographic analysis of hydrocarbons formed in bottom sediment samples by indigenous sulfate-reducing bacteria. The method proved sensitive enough to reliably establish the ability of sulfate-reducing bacteria to synthesize hydrocarbons in sediment samples incubated under CO2 + H2

The ability of sulfate-reducing bacteria of various taxonomic groups to synthesize extracellular hydrocarbons

Sulfate-reducing bacteria of various taxonomic groups were found to synthesize extracellular hydrocarbons during growth on lactate in an atmosphere of H2 + CO2. The most active producers of hydrocarbons were sulfate-reducing bacteria bringing about incomplete oxidation of lactate. Hydrocarbons produced were normal or isomeric alkanes with a chain length from C11 to C24. © 1997 MAHK Hayka/Interperiodica Publishing

Effect of gas phase composition on formation of hydrocarbons by Desulfovibrio desulfuricans | Vliianie sostava gazovoi fazy na obrazovanie uglevodorodov Desulfovibrio desulfuricans.

Changes in the synthesis of extracellular metabolic products generated by sulfate-reducing bacteria Desulfovibrio desulfuricans grown on a lactate-containing mineral medium in the presence of H2 and CO2 at various volume ratios in the gaseous phase were studied. An increase in the amount of extracellular products synthesized by the bacteria was observed at an H2/CO2 ratio of 3:1. High concentrations of molecular hydrogen (80-95%) in the presence of 5-20% CO2 facilitated the synthesis of hydrocarbons (alkanes) whose highest concentrations were produced at an H2/CO2 ratio of 9:1. An increase in the initial CO2 concentration in the gaseous phase above 20% increased the amount of oxygenated compounds in the culture

Comparative characterization of extracellular and intracellular hydrocarbons of Clostridium pasteurianum

Extracellular and intracellular hydrocarbons produced by Clostridium pasteurianum VKM 1774 during cultivation on glucose-containing media in an argon atmosphere or in the presence of carbon dioxide and molecular hydrogen were analyzed by gas-liquid chromatography. Intracellular hydrocarbons were 50-55% (C25-C35) n-alkanes. Carbon dioxide and molecular hydrogen stimulated synthesis of extracellular hydrocarbons, which comprised 90-95% (C11-C24) n-alkanes

Isolation and structural characterization of Rhizoctonia solani fungal lectin

In this paper was isolated and purified the lectin of Rhizoctonia solani according to the scheme, comprising the following stages: obtaining protein extract, salting out proteins by the crystalline ammonium sulfate, dialysis, ion exchange chromatography using Bio-Scale ™ Mini Macro-Prep High Q and DEAE-Sepharose columns, gel filtration on the column with Sephadex G-50. The most complete lectin removal was observed at 65% saturation of fungal mycelium buffer extract by ammonium sulfate in 12 hours of salt acting. Ion exchange chromatography by using Mini Macro-Prep High Q and DEAE-Sepharose columns allowed to increase the degree of lectin purification in 26.5 and 41.8 times, respectively, relative to the initial fraction of the glycoprotein. The highest degree of purification was obtained after gel filtration of R. Solani lectin using column with Sephadex G-50. As a result of the experiments there was obtained lectin preparation with 107.54% degree of purification and specific activity of 1.0×10 5 U/mg. The output on the protein activity was 17.2%. By electrophoresis method in denaturing conditions and by gel filtration using Sephadex G-100 it was revealed that the lectin of Rh. solani is a low molecular weight glycoprotein comprising two subunits of molecular weight 18.0 ± 1.5 kDa. The total molecular weight of the native protein is 36.0 ± 2.0 kDa

Comparative characterization of extracellular and intracellular hydrocarbons of Clostridium pasteurianum

Extracellular and intracellular hydrocarbons produced by Clostridium pasteurianum VKM 1774 during cultivation on glucose-containing media in an argon atmosphere or in the presence of carbon dioxide and molecular hydrogen were analyzed by gas-liquid chromatography. Intracellular hydrocarbons were 50-55% (C25-C35) n-alkanes. Carbon dioxide and molecular hydrogen stimulated synthesis of extracellular hydrocarbons, which comprised 90-95% (C11C24) n-alkanes

Effect of various organic compounds on the growth and hydrocarbon production by sulfur-reducing bacteria | Vliianie razlichnykh organicheskikh veshchestv na rost i obrazovanie uglevodorodov sul'fatredutsiruiushchimi bakteriiami.

The effects of lactate, pyruvate and ethanol on growth and formation of extracellular hydrocarbons by Desulfovibrio desulfuricans 1799 cultivated in H2 + CO2 atmosphere were studied. It was shown that sulfate reducing bacteria grow and produce hydrocarbons on all studied carbon and energy sources. Substitution of lactate in the medium for pyruvate or ethanol decreased only insignificantly the amount of synthesized hydrocarbons with concomitant increase in the content of isoform

Mycelial and extracellular lectins of lower fungi

Screening of 27 fungi belonging to Rhizoctonia, Fusarium, Aspergillus and Penicillum on their ability of mycelial and intracellular lectins biosynthesis was carried out. It was revealed that the majority of isolates synthesized lectins with different degree of activity. Micromycet Rh. solani stood out among other strains due to the pronounced ability to produce highly active mycelial lectins (titer of 16384). Extracellular lectins of studied strains possessed significantly lower agglutinative activity compared to lectins from mycelial extracts, or did not have it at all. The highest activity of extracellular lectins was observed in isolates of Rh.solani (titer 512) and A.flavus (titer 512). A lot of fungi lectins lacked specificity against red blood cells of 1-3 groups of human blood, but did not cause agglutination of red blood cells of sheep. The exception was mycelial lectins of F. sporotrichioides and Penicillum 4 isolates and an extracellular lectin of F. redolens 1 isolate, which showed specificity only to the 1st group of human blood and extracellular lectin of A. niger 2-to the 2 group. Surface modification of erythrocytes with trypsin or pronase significantly increased the ability of lectins to hemagglutination, and red blood cell pronase treatment was far more effective

Experience in using Aterixen® in clinical practice. Results of the "SUPRA" observation program

Background. The new medical product Aterixen (XC221GI) has appeared on the pharmaceutical market in 2022. It was revealed, at preclinical stage, that the main anti-inflammatory drug's effect was assured by its effect on production of anti-inflammatory cytokines IL-6, IL-8 and chemokines IP-10 (CXCL10), MIG (CXCL9). The results of double-blind randomized placebo-controlled studies have shown high efficacy of the drug in the management of patients with new coronavirus infection (COVID-19) of different severity. Aim. To evaluate experience of Atherixen practical use among physicians and general practitioners. Materials and methods. The observational program included patients aged from 18 to 60 years with confirmed diagnosis of mild COVID-19. All patients have signed voluntary informed consent to participate in the study. The study consisted of 2 periods: screening period and drug administration period. All patients received Aterixen (100 mg tablets), 1 tablet 2 times per day for 14 days within the standard therapy outlined in the Temporary methodological recommendations on prevention, diagnosis, and treatment of COVID-19. Efficacy was assessed by mean disease duration and physician and patient treatment satisfaction via five-point Likert scale. Results. The average disease duration did not exceed 9.5 days. It indicates the ability of Aterixen to prevent the transition of the disease to moderate and severe forms. The degree of physician and patient treatment satisfaction via five-point Likert scale in the vast majority of cases corresponded to the highest grades. Moreover, no adverse events were reported during the study and all patients had high tolerability

Isolation,selection and molecular identification of biosurfactant-producing extremophilicbacteries from crude oil polluted soil

© 2016,International Journal of Pharmacy and Technology. All rights reserved.Ten bacterial strains with hydrocarbon degrading capacity were isolated from a soil sample that had been polluted with crude oil from the area of Surgut in Russian Federation. This area is characterized by its long winters,with an annual average temperature of-1.7ºC. The isolated bacterial strains live and thrive at a very low temperature,which makes them ideal to be used in unfavorable environmental conditions for the majority of surfactants.In order to evaluate the biosurfactant production of these strains,the following methods were used: surface tension measurements,drop dispersion,hemolytic capacities assay and emulsification rate assay.These analysis showed that two of the strains,Bacillus subtilis and Klebsiellaoxytoca,was efficient biosurfactant producers. The surface tension decrease when using B.subtilisand K. oxytoca was 64.3% and 57.1% respectively. Drop dispersion was 33mm with B.subtilis and 28mm with K.oxytoca. The emulsification rate when using B.subtilis and K.oxytoca was 78.4% and 59.2% respectively. Our research has prospects to be applied both for microbial enhanced oil recovery (MEOR) and for bioremediation