Istraživanje patologije i dokaz koinfekcije goveđim papiloma virusima na koži i bradavicama sisa goveda

Bovine papillomas are benign tumors of the cutaneous and mucosal epithelia and are commonly found in cattle. The productivity loss and economic impact depends on the location and degree of infection. The present study was undertaken to investigate the pathology and association of different bovine papilloma virus (BPV) types in cattle cutaneous and teat warts. Grossly, the warts were of variable size and shape (rice grain, cauliflower and finger- like horny growths or irregular). Histopathologically, the warts were diagnosed as fibropapilloma, papilloma, fibrosarcoma and hyperplasia. Fibropapilloma was the most frequent histological type observed and was characterized by hyperkeratosis, parakeratosis and acanthosis. PCR revealed the presence of either BPV-1, -2 and -5 DNA or their co-infections. Transmission electron microscopy on negative staining showed BPV-like particles. Varied copy numbers of viral DNA of BPV-1, -2 and -5 were detected by real-time PCR. Immunohistochemistry revealed the expression of Ki-67 mainly in the proliferating cells of stratum spinosum and a few basal cells in papilloma and fibropapilloma. Cyclooxygenase-2 immunostaining was observed in the cytoplasm and cell membrane of suprabasal cells. In conclusion, cutaneous and teat warts in cattle in India are more frequently associated with BPV-1/ -2 and their mixed infections, with the rare presence of BPV-5. The DNA of BPV-5 was detected for the first time in warts in India. Co-infection with two or three different viral types demonstrated the diversity of BPV types involved in warts. The frequent expression of Ki-67 in suprabasal layers may be indicative of its association with viral replication and that they are as proliferation sites.Papilomi su benigni tumori kožnog i sluzničnog epitela koji se u goveda često nalaze. Proizvodni gubici i utjecaj na ekonomičnost uzgoja ovise o lokaciji i stupnju infekcije. Ovo je istraživanje provedeno kako bi se ustanovila patologija i povezanost različitih tipova goveđih papilomavirusa (BPV) na koži i bradavicama sisa goveda. Bradavice su većinom bile različite veličine i oblika (oblika zrna riže, cvjetače i rožnate izrasline nalik na prste ili nepravilna oblika). Histopatološki su bradavice dijagnosticirane kao fibropapilomi, papilomi, fibrosarkomi i hiperplazije. Fibropapilomi su bili najčešći histološki tip, obilježeni hiperkeratozom, parakeratozom i akantozom. PCR-om je dokazan DNA virusa BPV-1, BPV-2 i BPV-5 DNA ili koinfekcije tim virusima. Negativno bojenje elektronskom transmisijskom mikroskopijom pokazalo je čestice nalik na BPV. PCR-om u stvarnom vremenu otkriveni su različiti brojevi kopija virusne DNA virusa BPV-1, BPV-2 i BPV-5. Imunohistokemijskim je pretragama pronađena ekspresija Ki-67, većinom u proliferativnim stanicama stratum spinosum i nekoliko bazalnih stanica u papilomima i fibropapilomima. Provedeno je imunobojenje ciklooksigenazom 2 u citoplazmi i staničnoj membrani suprabazalnih stanica. Zaključeno je da su u Indiji bradavice na koži i sisama u goveda mnogo češće povezane s BPV-1 i BPV-2 i mješovitim infekcijama tim virusima, dok je prisutnost virusa BPV-5 rijetka. DNA virusa BPV-5 prvi je put pronađena u bradavicama u Indiji. Koinfekcije dvama ili trima različitim virusnim tipovima pokazuje raznolikost tipova BPV-a koji se nalaze u bradavicama. Učestala ekspresija Ki-67 u suprabazalnim slojevima može upućivati na njegovu povezanost s virusnom replikacijom i mjestima proliferacije

Kliničko-patološko istraživanje i liječenje neuobičajenog slučaja sarkoptes-šuge kunića i mačke

Mites cause several skin conditions, such as demodectic, sarcoptic, psoroptic and notoedric mange. This study presents the clinicopathological findings of sarcoptic mange in a rabbit and cat, and its therapeutic management. One non-descript male rabbit and one female cat, housed together in the same premises were presented to the Veterinary Clinical Complex, Lala Lajpat Rai University of Veterinary and Animal Sciences, Hisar with a history of alopecia, anorexia, intense itching, erythema, dandruff and whitish dry crust-like lesions on their ears, nose, face, and the areas surrounding the ears and eyes. Microscopic examination of skin scrapings from different sites confirmed sarcoptic mange. They were successfully treated with ivermectin and fipronil spray in intervals of 15 days, along with supportive therapy.Grinje uzrokuju nekoliko kožnih bolesti među kojima se, ovisno o uzročniku, ističu demodeks-šuga, sarkoptes-šuga, psoroptes-šuga i notoedres-šuga. U radu su prikazani kliničko-patološki nalazi i liječenje neuobičajenog slučaja sarkoptes-šuge kunića i mačke. Mužjak kunića i mačka smješeteni su zajedno u istim prostorijama veterinarske klinike Fakulteta za veterinarske i animalne znanosti, Sveučilišta Lala Lajpat Rai, Hisar. U anamnezi oboljelih životinja su navedeni alopecije, anoreksija, intenzivni svrbež, eritem, perut te bjelkaste, suhe, krastolike lezije na i u okolini ušiju, nosa i lica. Mikroskopski pregled strugotina kože s različitih mjesta pokazao je odrasle parazite iz porodice Sarcoptidae i njihova jajašca. Životinje su tijekom razdoblja od 15 dana, uz potpornu terapiju, uspješno liječene ivermektinom i fipronil sprejom