Dietary Fibre and Micronutrient Potential of Underutilized Green Leafy Vegetable Sprinkles on Selected Foods

Considering the rural poor in developing countries like Nigeria, diets that are deficient in dietary fibre and micronutrients are common. Food  diversification or dietary modifications such as increased consumption of vegetables may solve the problem. This study assessed the dietary fibre and micronutrient levels of selected starchy foods (jollof rice and yam porridge) using three underutilized green leafy vegetables, namely, Ebolo (Crassocephalum crepidiodes), Odu (Solanum nigrum L), and Yanrin (Launaea teracifolia L) as sprinkles. Fresh samples of Crassocephalum crepidioides, Solanum nigrum L, and Launaea teracifolia were purchased from Ota market. The leafy parts of the vegetables were washed, cut, oven-dried at 90°C for 6 h and pulverized. Jollof rice and porridge were cooked and 2g each of dried ebolo, odu and yanrin were sprinkled on 100g of each food and stirred thoroughly. Total dietary fibre, as well as iron (Fe) and Zinc (Zn) content of the resultant diets, were determined using standard methods. The highest total dietary fibre of 8.45% was obtained in jollof rice sprinkled with Yanrin. Zn (31.75mg/kg) was highest in jollof rice sprinkled with Yanrin, and Fe (87.75mg/kg) in porridge sprinkled with Ebolo. The underutilized vegetables added more nutritional values to jollof rice and yam porridge as Ebolo, Yanrin and Odu were sprinkled on them. Keywords: Underutilized vegetables, dietary fibre, micronutrient, zinc, iro

Preliminary in vitro screening of some spices and medicinal plants from Edo and Rivers States, Nigeria for reducing enteric methane production in ruminants

Methane gas produced by ruminants during feed fermentation contributed to global warming as well as poor efficiency of food utilization.  Mitigating ruminal methane production through anti-microbial feed additive has serious health implications. A preliminary study of the potential of medicinal plants and spices from Edo and Rivers State, as possible replacement to antibiotic feed additives was carried out. Medicinal plants and  spices were purposefully sampled, dried at 40oC, milled and 10 mg was added with 190 mg of substrate in a preweighed nylon incubation bags. Bags and their contents were incubated using 30 mL of buffered rumen liquor in 100 mL syringes following the standard procedure of in vitro fermentation. Variables monitored were 24h total gas volume, short chain fatty acids (SCFA), methane (CH ) gas, dry matter degradability (DMD) as well as some chemical, cell wall and 4 anti-nutritive components. Results revealed that adding the medicinal plants and spices as feed additives did not depress gas production in most cases (19.5 – 29.50 mL, 17.75 – 30.00 mL and 12.00 – 29.00 mL for Edo medicinal plants, Edo spices and Rivers  medicinal plants respectively) and also did not depress DMD (> 50%). Azadirachta indica stem reduced CH4 production mostly by 53.56% while Dennttia tripetala did by 47.69% among the Edo medicinal plants and spices, respectively. In Rivers State, Amaranthus spinosus gave the highest value of 60.33% CH reduction. In the same bracket of similar and good CH4 reduction potentials were Alstonia boonei leaves (49.06%),  Newbouldia laevis root (41.50%), Euphorbia heterophyla (37.97%), Allium crispum (47.31%), Allium cepa (46.80%), Aframomum mueguata (46.54%), Capsicum annum (47.32%), Ageratum conyzoides (56.52%), Lagenaria breviflora (48.03%) and Centrosema molle (45.08%). All the medicinal plant and spices with potential for CH4 reduction had corresponding high SCFA 4 values (0.46 – 0.65 Mmol) with tannin (%) and saponin (%) contents of  0.011 – 17.50 and 0.01 – 2.40 respectively. Conclusively, the medicinal plants and spices demonstrated good potentials for reducing rumen methane production in vitro and may be subjected to further in vivo studies. Keywords: in vitro, spices, medicinal plants, methane production, ruminant