Distribution and penetration of reactive oxygen and nitrogen species through a tissue phantom after Plasma Gun treatment

International audienceThe transport and distribution phenomena of reactive oxygen and nitrogen species (RONS) into biological tissue following non-thermal plasma treatment have received much attention. The aim of this study is to test the efficacy of Plasma Gun (PG) on transporting the reactive species in an agar composed tissue phantom. RONS are generated on the agar gel by the plasma treatment and they continue to spread in the depth after plasma exposure. The amount of RONS after passing through the tissue phantom are strongly depending on discharge parameters such as distance to the target and capillary shape

Fluorescein-agarose gel as a tissue model to visualize and measure local CAP-induced acidification

International audienceCold Atmospheric Plasmas (CAPs) have been shown to lower the pH of the treated target. CAP-induced acidification can have beneficial effects on the treated tissue (e.g. disinfection and healing). Conversely a tissue hyper acidification can lead to permanent damages with dramatic consequences. Before starting in vivo plasma treatments, preliminary tests should be carried in order to tune the acidifying effect induced by CAP exposition. Here we propose a tissue model that can be a useful tool to visualize and measure pH changes induced by plasma treatment

HPV16 E7-Dependent Transformation Activates NHE1 through a PKA-RhoA-Iinduced Inhibition of p38alpha

Background: Neoplastic transformation originates from a large number of different genetic alterations. Despite this genetic variability, a common phenotype to transformed cells is cellular alkalinization. We have previously shown in human keratinocytes and a cell line in which transformation can be turned on and followed by the inducible expression of the E7 oncogene of human papillomavirus type 16 (HPV16), that intracellular alkalinization is an early and essential physiological event driven by the up-regulation of the Na/H-+(+) exchanger isoform 1 (NHE1) and is necessary for the development of other transformed phenotypes and the in vivo tumor formation in nude mice.Methodology: Here, we utilize these model systems to elucidate the dynamic sequence of alterations of the upstream signal transduction systems leading to the transformation-dependent activation of NHE1.Principal Findings: We observe that a down-regulation of p38 MAPK activity is a fundamental step in the ability of the oncogene to transform the cell. Further, using pharmacological agents and transient transfections with dominant interfering, constitutively active, phosphorylation negative mutants and siRNA strategy to modify specific upstream signal transduction components that link HPV16 E7 oncogenic signals to up-regulation of the NHE1, we demonstrate that the stimulation of NHE1 activity is driven by an early rise in cellular cAMP resulting in the down-stream inhibition of p38 MAPK via the PKA-dependent phosphorylation of the small G-protein, RhoA, and its subsequent inhibition.Conclusions: All together these data significantly improve our knowledge concerning the basic cellular alterations involved in oncogene-driven neoplastic transformation

Sustainable Development Goals: Integrating sustainable initiatives with long-term value creation

Our planet continues to face many global economic, social, and environmental challenges and uncertainties. To help deal with them, in September 2015 many governments worldwide agreed to pursue 17 Sustainable Development Goals (SDGs). Promoted by the United Nations, these SDGs define global priorities and aspirations for 2030 and rely on the important and value-creating role of business organizations in delivering on the promise of sustainable and inclusive development. SDGs will be both an opportunity and a challenge in the years ahead. Several business organizations across the globe have started this journey by identifying and executing sustainable strategies as key drivers of their visions and business models. The SDGs present an opportunity for business-led solutions and technologies to be developed, and they offer an overarching framework to shape, guide, measure, and report the value created through business objectives, initiatives, and performance. Measuring and reporting on these goals enable business organizations to contribute to the SDGs while capitalizing on a range of benefits such as identifying future business opportunities and strengthening stakeholder engagement. What are the Sustainable Development Goals? Where do they come from? Are companies ready to engage with them? How? What are the possible roles of management accountants in this space? We address (and perhaps answer) these questions by providing examples of a number of organizations—such as PepsiCo and Eni—that have been pioneering a certain degree of attention to the SDGs when measuring and reporting their business performances

Etude du stress oxydant dans les conditions réelles d'oxygénation de la peau : la physioxie.

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Study of oxydative stress under the true oxygenation of the skin, physioxia

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Rôle du taux d’oxygénation sur l’activité antioxydante des cellules épidermiques

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