Study of mast cell density, distribution and morphology in skin lesions of leprosy

Background: Mast cells are found at all levels of dermis, grouped around blood vessels, nerves and appendages. An increase in the mast cell number has been reported in various cutaneous diseases. Mast cells respond to diverse range of stimuli like neuronal impulses, allergens, antigens, hormones, cytokines from T cells and keratinocytes and thus play a role in manifestation of dermatological disorders. The objective was to study mast cell density in various subsets of Hansen’s disease and analyse whether the distribution and density vary amongst the subsets. Methods: A total of 60 skin biopsies diagnosed as leprosy were included in this study. 10 control slides were also studied for mast cell density and distribution. Histopathological diagnosis was made on the biopsy and mast cell density and distribution was assessed on toluidine blue stained sections and compared with those of controls. Results: All the subsets of Hansen’s disease studied showed an increase in the number of mast cells. The highest mean mast cell count was noted in lepromatous leprosy (66/mm2), followed by erythema nodosum leprosum (42.6/mm2) and borderline lepromatous leprosy (40.8/mm2). The mean mast cell count showed progressive increase over the immunological spectrum of tuberculoid leprosy to lepromatous leprosy. The mast cells showed spindle and ovoid morphology in all the lesions (active) while round morphology was seen in controls. Conclusion: Tissue mast cell number, distribution and morphology vary depending on the degree of activation in different leprosy lesions. This may have a bearing on the diagnosis and management

Functional hard-boiled candy formulation employing Plackett Burman design

Plackett-Burman experimental design was employed as a screening experiment with five variables for preparation of hard boiled candy. The effect of varying the levels of hard-boiled candy ingredients such as ratio of liquid glucose (30-40%): sugar (60-70%) addition of nutraceuticals namely green tea extract (0.5-1%) and Amla powder (0.5-1%) alongwith salt (0.1 to 0.2%) on the physicochemical responses such as hardness, colour, total polyphenol, antioxidant radical scavenging, vitamin C and overall quality were studied. Results indicated that a formulation of: sugar 70%, liquid glucose 30%, amla powder and green tea extract 1% and salt 0.1% was considered as the optimum for obtaining a hard-boiled candy (HBC) with highest overall quality. The present study showed that the hard-boiled candy with added green tea extract and Amla powder were natural sources of polyphenols, vitamin C and antioxidants

Frequency Offset Locked, Dual Carrier Excitation of Phase modulated, Electro-optic Frequency Combs for Bandwidth Scaling and Nonlinear Spectral Broadening

DWDM with/without superchannel based photonic networks require the use of optical carriers with equalised amplitudes and frequency stabilization of adjacent carriers to realise reliable high bandwidth optical communication systems with high spectral efficiency and long reach. Cascading of electro-optic (EO) modulators is a versatile method for generating tuneable, high repetition rate frequency combs which can be used as sources for the carriers. However, the number of lines produced with this technique is limited by the number of phase modulators. Nonlinear spectral broadening is an attractive option for bandwidth scaling; however, bandwidth scaling of single carrier combs through four wave mixing suffers from unequal comb lines or power limitations due to Brillouin scattering. A simpler technique to increase the number of comb lines would involve using multicarrier excitations for comb generation which would result in a proportional increase in the comb lines. Further, dual-carrier excitation enables an excellent temporal profile for nonlinear spectral broadening. However, since the two carriers have uncorrelated drifts, the resultant frequency combs would be unsuitable for most applications. This issue can be overcome by frequency offset locking the two lasers. Here, we demonstrate frequency offset locking (MHz accuracy) of two diode lasers spaced by 100GHz by using an optical phase locked loop which locks one laser to a RF harmonic of the other. This allows for the generation of frequency comb lines locked to each other even post nonlinear broadening. Using this technique, we demonstrate a 25GHz frequency comb with >90 lines (2THz) in the C-band

A scalable, self-sustaining model for screening and treatment of diabetic retinopathy in rural Karnataka.

The Indian health infrastructure is struggling to handle the burgeoning number of people with diabetes. Managing the complications of diabetes in an organized manner through the government health programs is still a distant reality. Here, we describe a program aimed at addressing the problem of diabetic retinopathy in rural areas of Tumkur district in Karnataka. By amalgamating telescreening and our own novel distributive care model, we were able to screen 85% of the registered diabetics in the Government noncommunicable disease clinics and treat 95% of those needing laser therapy. We also describe the importance of using electronic medical records in public health programs which not only increase the efficiency in screening for disease but help in increasing uptake of treatment by tracking defaulters