Effects of aeration and mineral supply on growth and mineral content of shoots and roots of apple trees (var. 'Golden Delicious' on M.IX).

One- or 2-year-old Golden Delicious apple trees on M.IX were grown either in a clay loam (in 60-1 containers) or in nutrient solution and the effects were studied of several aeration regimes. With the soil-grown trees these comprised flooding to various soil depths and for different times. With the trees grown in nutrient solution the aeration regimes compared were nutrient solution without aeration, tap water with or without aeration, and aerated nutrient solution (control). Shoot growth was depressed by high water tables, and that of trees receiving non-aerated nutrient solution or tap water (whether aerated or not) ceased after about 2 weeks. The root growth of trees receiving non-aerated nutrient solution or tap water was similarly depressed, but, by contrast, aerated tap water enhanced root growth. Details are given of treatment effects on the Ca, K, N and soluble sugar contents of the young and old leaves and of the young roots. The uptake of all mineral elements was similarly affected by lack of aeration, suggesting that the relationship between low Ca content and increased bitter pit incidence is not associated with differential absorption but rather with differential Ca distribution. (Abstract retrieved from CAB Abstracts by CABI’s permission

Treatment of Chronic Hepatitis C: the Benelux Studies

__Abstract__ In the eighties and early nineties of the last century, acute hepatitis occurred in 5-10% of patients receiving blood transfusions in the USA, and in more than 90% of cases this could not be attributed to hepatitis A or B (“Non-A, Non-B hepatitis”). More than 50% of the hepatitis infections became chronic, and Non-A, Non-B hepatitis led to liver cirrhosis in about 20% of patients, thereby being a serious health burden. In 1989 the hepatitis C virus was discovered as the major cause of this post-transfusion hepatitis, but it was also found in many cases of unknown chronic hepatitis without known blood contacts in the past (sporadic HCV). It is estimated that more than 170 million people are infected with hepatitis C worldwide, leading to more than 280.000 deaths each year due to decompensated liver cirrhosis and liver cancer

Changes in anti-viral effectiveness of interferon after dose reduction in chronic hepatitis C patients: a case control study

BACKGROUND: High dose interferon induction treatment of hepatitis C viral infection blocks viral production over 95%. Since dose reduction is often performed due to clinical considerations, the effect of dose reduction on hepatitis C virus kinetics was studied. METHODS: A new model that allowed longitudinal changes in the parameters of viral dynamics was used in a group of genotype-1 patients (N = 15) with dose reduction from 10 to 3 million units of interferon daily in combination with ribavirin, in comparison to a control group (N = 9) with no dose reduction. RESULTS: Dose reduction gave rise to a complex viral kinetic pattern, which could be only explained by a decrease in interferon effectiveness in blocking virion production. The benefit of the rapid initial viral decline following the high induction dose is lost after dose reduction. In addition, in some patients also the second phase viral decline slope, which is highly predictive of success of treatment, was impaired by the dose reduction resulting in smaller percentage of viral clearance in the dose reduction group. CONCLUSIONS: These findings, while explaining the failure of many induction schedules, suggest that for genotype-1 patients induction therapy should be continued till HCVRNA negativity in serum in order to increase the sustained response rate for chronic hepatitis C

Sequence analysis of the 5' untranslated region in isolates of at least four genotypes of hepatitis C virus in The Netherlands

The RNAs of hepatitis C virus (HCV) isolates from 62 patients with chronic HCV infection were analyzed by direct sequencing of the 5' untranslated region. Two important sequence motifs were recognized: one between positions -170 and -155 and the other between positions -132 and -117. These motifs are partly complementary. All three previously published genotypes were observed; 34 (55%) isolates were classified as type 1 (including prototype [from the United States] and HCV-BK [from Japan] sequences), 11 (18%) were classified as type 2 (including HC-J6 and HC-J8), and 12 (19%) were classified as type 3 (including EB1); one patient was infected with genotypes 1 and 2. Four (6%) isolates showed aberrant sequences and were therefore provisionally classified as genotype 4. These results indicate the significance of sequence variation among the 5' untranslated regions of different HCV genotypes and indicate that this region could possibly be used for consistent genotyping of HCV isolates

Rapid genotyping of hepatitis C virus RNA-isolates obtained from patients residing in Western Europe

Two rapid genotyping methods for hepatitis C virus (HCV), the line probe assay (Inno‐LiPA) and the subtype‐specific core amplification system [Okamoto et al., (1992b) Journal of General Virology 73:673‐679], were applied to 58 HCV isolates which were typed as type 1 (n=37) and type 2 (n=21) by sequence analysis of the 5′ untranslated region (5′UTR). The line probe assay targets the 5′UTR and recognized 12 subtype 1a, 25 subtype 1b, 18 subtype 2a, 2 subtype 2b and 1 subtype 2d in accordance with sequence analysis of this region. Subtype‐specific core amplification revealed 7 discrepancies among the 37 type 1 isolates when compared to LiPA. A different subtype was observed in 3 isolates (la versus 1b), 2 isolates remained untyped and 2 isolates showed a coinfection of subtype la and 1b. The first 5 discrepancies were confirmed by sequence analysis of the core region whereas the coinfection could not be confirmed. Of the 21 type 2 isolates only one could be typed by subtype‐specific core amplification. HCV RNA was detected in all 21 cases after the general first round of polymerase chain reaction (PCR). Direct sequencing of the core region indicated sequence variation as a source of failure. It is concluded that LiPA results are conclusive for typing of HCV. However, LiPA is hampered occasionally for subtyping by lack

Prospective comparative study of spiral computer tomography and magnetic resonance imaging for detection of hepatocellular carcinoma

BACKGROUND: Hepatocellular carcinoma (HCC) is often detected at a relatively late stage when tumour size prohibits curative surgery. Screening to detect HCC at an early stage is performed for patients at risk. AIM: The aim of this study was to compare prospectively the diagnostic accuracy and classification for management of the two state of the art secondline imaging techniques: triphasic spiral computer tomography (CT) and super paramagnetic iron oxide (SPIO) enhanced magnetic resonance imaging (MRI). PATIENTS: Sixty one patients were evaluated between January 1996 and January 1998. Patients underwent CT and MRI within a mean interval of 6.75 days. METHODS: CT and MRI were evaluated blindly for the presence and number of lesions, characterisation of these lesions, and classification for management. For comparison of the data on characterisation, the CT and MRI findings were compared with histopathological studies of the surgical specimens and/or follow up imaging. Data of patients not lost to follow up were available to January 2001. RESULTS: SPIO enhanced MRI detected more lesions and overall smaller lesions than triphasic spiral CT (number of lesions 189 v 124; median diameter 1.0 v 1.8 cm; Spearman rank's correlation coefficient 0.63, p<0.001). There was no significant difference in accuracy between CT and MRI for lesion characterisation. The agreement in classification for management was very good (weighted kappa 0.91, 95% CI 0.83-0.99). CONCLUSION: SPIO enhanced MRI detects more and smaller lesions, but both techniques are comparable in terms of classification for management. SPIO enhanced MRI may be preferred as there is no exposure to ionising radiation

Absence of a metallic phase in random-bond Ising models in two dimensions: applications to disordered superconductors and paired quantum Hall states

When the two-dimensional random-bond Ising model is represented as a noninteracting fermion problem, it has the same symmetries as an ensemble of random matrices known as class D. A nonlinear sigma model analysis of the latter in two dimensions has previously led to the prediction of a metallic phase, in which the fermion eigenstates at zero energy are extended. In this paper we argue that such behavior cannot occur in the random-bond Ising model, by showing that the Ising spin correlations in the metallic phase violate the bound on such correlations that results from the reality of the Ising couplings. Some types of disorder in spinless or spin-polarized p-wave superconductors and paired fractional quantum Hall states allow a mapping onto an Ising model with real but correlated bonds, and hence a metallic phase is not possible there either. It is further argued that vortex disorder, which is generic in the fractional quantum Hall applications, destroys the ordered or weak-pairing phase, in which nonabelian statistics is obtained in the pure case.Comment: 13 pages; largely independent of cond-mat/0007254; V. 2: as publishe

Network models for localisation problems belonging to the chiral symmetry classes

We consider localisation problems belonging to the chiral symmetry classes, in which sublattice symmetry is responsible for singular behaviour at a band centre. We formulate models which have the relevant symmetries and which are generalisations of the network model introduced previously in the context of the integer quantum Hall plateau transition. We show that the generalisations required can be re-expressed as corresponding to the introduction of absorption and amplification into either the original network model, or the variants of it that represent disordered superconductors. In addition, we demonstrate that by imposing appropriate constraints on disorder, a lattice version of the Dirac equation with a random vector potential can be obtained, as well as new types of critical behaviour. These models represent a convenient starting point for analytic discussions and computational studies, and we investigate in detail a two-dimensional example without time-reversal invariance. It exhibits both localised and critical phases, and band-centre singularities in the critical phase approach more closely in small systems the expected asymptotic form than in other known realisations of the symmetry class.Comment: 14 pages, 15 figures, Submitted to Physical Review

Bosonic Excitations in Random Media

We consider classical normal modes and non-interacting bosonic excitations in disordered systems. We emphasise generic aspects of such problems and parallels with disordered, non-interacting systems of fermions, and discuss in particular the relevance for bosonic excitations of symmetry classes known in the fermionic context. We also stress important differences between bosonic and fermionic problems. One of these follows from the fact that ground state stability of a system requires all bosonic excitation energy levels to be positive, while stability in systems of non-interacting fermions is ensured by the exclusion principle, whatever the single-particle energies. As a consequence, simple models of uncorrelated disorder are less useful for bosonic systems than for fermionic ones, and it is generally important to study the excitation spectrum in conjunction with the problem of constructing a disorder-dependent ground state: we show how a mapping to an operator with chiral symmetry provides a useful tool for doing this. A second difference involves the distinction for bosonic systems between excitations which are Goldstone modes and those which are not. In the case of Goldstone modes we review established results illustrating the fact that disorder decouples from excitations in the low frequency limit, above a critical dimension $d_c$, which in different circumstances takes the values $d_c=2$ and $d_c=0$. For bosonic excitations which are not Goldstone modes, we argue that an excitation density varying with frequency as $\rho(\omega) \propto \omega^4$ is a universal feature in systems with ground states that depend on the disorder realisation. We illustrate our conclusions with extensive analytical and some numerical calculations for a variety of models in one dimension

Hidden degree of freedom and critical states in a two-dimensional electron gas in the presence of a random magnetic field

We establish the existence of a hidden degree of freedom and the critical states of a spinless electron system in a spatially-correlated random magnetic field with vanishing mean. Whereas the critical states are carried by the zero-field contours of the field landscape, the hidden degree of freedom is recognized as being associated with the formation of vortices in these special contours. It is argued that, as opposed to the coherent backscattering mechanism of weak localization, a new type of scattering processes in the contours controls the underlying physics of localization in the random magnetic field system. In addition, we investigate the role of vortices in governing the metal-insulator transition and propose a renormalization-group diagram for the system under study.Comment: 17 pages, 16 figures; Figs. 1, 7, 9, and 10 have been reduced in quality for e-submissio