337 research outputs found
Microbiota of Deciduous Endodontic Infections Analyzed by MDA and Checkerboard DNA-DNA Hybridization
Aims To evaluate the microbiota of endodontic infections in deciduous teeth by checkerboard DNA-DNA hybridization after uniform amplification of DNA in samples by multiple displacement amplification (MDA). Methodology Forty samples from the root canal system of deciduous teeth exhibiting pulp necrosis with or without radiographically detectable periradicular/interadicular bone resorption were collected and 32 were analyzed, with 3 individuals contributing 2 samples; these were MDA- amplified and analyzed by Checkerboard DNA-DNA hybridization for levels of 83 bacterial taxa. Two outcome measures were used: the percentage of teeth colonized by each species; and the mean proportion of each bacterial taxon present across all samples were computed. Results The mean amount of DNA in the samples prior to amplification was 5.2 (± 4.7) ng and 6.1 (± 2.3) ÎŒg after MDA. The mean number of species detected per sample was 19 (± 4) (range: 3â66) to the nearest whole number. The most prevalent taxa were Prevotella intermedia (96.9%), Neisseria mucosa (65.6%), Prevotella nigrescens (56.2%) and Tannerella forsythia (56.2%). Aggregatibacter (Haemophilus) aphrophilus and Helicobacter pylori were not detected. P. intermedia (10%), Prevotella tannerae (7%) and Prevotella nigrescens (4.3%) presented the highest mean proportions of the target species averaged across the positive samples. Conclusion Root canals of infected deciduous teeth had a diverse bacterial population. Prevotella sp were commonly found with P. intermedia, Prevotella tannerae and Prevotella nigrescens among the most prominent species detected
Regularization Independent Analysis of the Origin of Two Loop Contributions to N=1 Super Yang-Mills Beta Function
We present a both ultraviolet and infrared regularization independent
analysis in a symmetry preserving framework for the N=1 Super Yang-Mills beta
function to two loop order. We show explicitly that off-shell infrared
divergences as well as the overall two loop ultraviolet divergence cancel out
whilst the beta function receives contributions of infrared modes.Comment: 7 pages, 2 figures, typos correcte
Efeito da idade do touro e do perĂodo de colheita de sĂȘmen sobre as caracterĂsticas fĂsicas e morfolĂłgicas do sĂȘmen de bovinos de raças europĂ©ias e zebuĂnas
Ordering and Fluctuation of Orbital and Lattice Distortion in Perovskite Manganese Oxides
Roles of orbital and lattice degrees of freedom in strongly correlated
systems are investigated to understand electronic properties of perovskite Mn
oxides such as La_{1-x}Sr_{x}MnO_{3}. An extended double-exchange model
containing Coulomb interaction, doubly degenerate orbitals and Jahn-Teller
coupling is derived under full polarization of spins with two-dimensional
anisotropy. Quantum fluctuation effects of Coulomb interaction and orbital
degrees of freedom are investigated by using the quantum Monte Carlo method. In
undoped states, it is crucial to consider both the Coulomb interaction and the
Jahn-Teller coupling in reproducing characteristic hierarchy of energy scales
among charge, orbital-lattice and spin degrees of freedom in experiments. Our
numerical results quantitatively reproduce the charge gap amplitude as well as
the stabilization energy and the amplitude of the cooperative Jahn-Teller
distortion in undoped compounds. Upon doping of carriers, in the absence of the
Jahn-Teller distortion, critical enhancement of both charge compressibility and
orbital correlation length is found with decreasing doping concentration. These
are discussed as origins of strong incoherence in charge dynamics. With the
Jahn-Teller coupling in the doped region, collapse of the Jahn-Teller
distortion and instability to phase separation are obtained and favorably
compared with experiments. These provide a possible way to understand the
complicated properties of lightly doped manganites.Comment: 22 pages RevTeX including 25 PS figures, submitted to Phys.Rev.B,
replaced version; two figures are replaced by Fig.17 with minor changes in
the tex
Thrips species (Insecta: Thysanoptera) associated with flowers in a restinga fragment in northeastern Brazil
A Biosensor Using Poly(4-Aminophenol)/acetylcholinesterase modified graphite electrode for the detection of dichlorvos
Efeito de diferentes proporçÔes de resĂduo Ășmido de cervejaria sobre as caracterĂsticas da carcaça de cordeiros terminados em confinamento
Characterization of potential CO2 emissions in agricultural areas using magnetic susceptibility
Etiology, antimicrobial susceptibility profile of Staphylococcus spp. and risk factors associated with bovine mastitis in the states of Bahia and Pernambuco
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