Evaluation of a Virus-like Replicon Particle Vaccine Expressing Proteins of Swine Influenza Virus in Pigs With and Without Maternally Derived Antibodies

A major hurdle to swine influenza vaccination of young piglets is maternal antibody interference. This interference is transient as it disappears when pigs reach about 3 months of age. We vaccinated piglets without and with interfering maternal antibody using a recombinant vector vaccine. In the absence of interfering maternal antibody, the vaccine was effective in inducing a strong immune response and greatly reduced the amount of virus. However, this same recombinant vaccine was not effective when interfering maternal antibodies were present. We are currently trying a higher dose of vaccine and different genes from SIV in hopes we can overcome this maternal antibody. Preliminary data from these new studies are promising

Quantitative Real-time PCR (qPCR) for the Determination of Salmonella Levels in Lairage

The sensitivity of the quantitative polymerase chain reaction assay (qPCR) was shown to be 10,000 Salmonella colony forming units (cfu) of bacteria per gram of fecal matter; this means that this rapid and specific test is a useful tool for the industry. The study found a trend toward a reduced, but not statistically significant, amount of Salmonella in lairage pens from standard hygiene practices of washing pens between groups of swine. This reduction of Salmonella in lairage pens may result in decrease transmission between groups, and, ultimately, in fewer numbers of contaminated carcasses at slaughter. It might also allow one to determine if on farm and transport methods are reducing Salmonella fecal incidence in lairage pens. The lack of significance is partially due to the low number of positive pens in this study before washing. Due to the low numbers found in pens in this study, samples were not collected from transport as they would have not lead to significant differences between various treatments

The wage utilisation relationship and the structure of labour costs

SIGLELD:6227.743(65) / BLDSC - British Library Document Supply CentreGBUnited Kingdo

Shiftworking and perceived working conditions An empirical analysis

LD:6227.743(66) / BLDSC - British Library Document Supply CentreSIGLEGBUnited Kingdo