Inactivation of potassium-evoked adrenomedullary catecholamine release in the presence of calcium, strontium or BAY-K-8644

AbstractThe rate of catecholamine release from cat adrenal glands perfused with Krebs solution containing 59 mM K declined exponentially during the first few minutes of depolarization. The rate of decline was considerably slower when Ca was substituted by Sr. The late addition of Ca, Sr or the Ca-channel activator BAY-K-8644 evoked a revival of secretion when catecholamine release was inactivated by prior K depolarization; the revival of secretion was independent of the depolarization time. These data demonstrate that inactivation of catecholamine release is specifically dependent on Ca; the modulatory role of Ca on secretion seems to be exerted at a step distal to the transmembraneous Ca channel

ORQUIECTOMIA PARA A REDUÇÃO DO VOLUME PROSTÁTICO. ESTUDO EXPERIMENTAL EM CÃES

Prostatic disorders like benign  prostatic hyperplasia,  prostatitis, cysts,  and neoplasia are common in older male dogs. The objective of the treatment of such disorders  is to decrease prostatic size aiming to alliviate  their respective symptoms. The aim of the present research work was to evaluate the efficacy of castration in  regard to the prostatic size and stromal tissue of  healthy dogs. The prostate volume was evaluated by  ultrasonography and the measurements performed before (baseline) orchiectomy and at 7, 21, 40 and 90 days after castration. Canine prostatic volumes significantly decreased by 81% in the 90th day after castration. In most of the experimental animals (80%) as much  as 50%  reduction of the prostate volume  has been observed  after  15 days of  castration, showing that orchiectomy is an efficient  therapy to reduce prostatic size. On the other hand, ultra-sonography is a non invasive diagnostic method indicated towards prostatic evaluation.Doenças prostáticas como a hiperplasia prostática benigna (HPB), prostatites, cistos e neoplasias são enfermidades comuns em machos com idade avançada. O aumento prostático promove compressão do reto e uretra causando tenesmo, constipação, disúria e anúria; o tratamento mais eficaz para reduzir o tamanho prostático, principalmente na HPB, é a castração. Este estudo objetivou avaliar as dimensões e a estrutura prostática após a orquiectomia em cães normais; além da eficiência da ultra-sonografia. O volume prostático de 10 cães foi avaliado por meio da ultra-sonografia transabdominal antes e aos 7, 21, 40 e 90 dias após a orquiectomia. A análise dos resultados demonstrou que houve redução significativa de 81% do volume prostático 90 dias após a castração, sendo que a maioria dos cães (80%) apresentou pelo menos 50% de redução do volume prostático após 15 dias da orquiectomia. A orquiectomia é uma terapia eficiente para a redução do volume prostático. O emprego da ultra-sonografia transabdominal é um método não invasivo adequado para avaliação e mensuração prostática em cães

Efficacy of direct current generated by multiple-electrode arrays on F3II mammary carcinoma: experiment and mathematical modeling

BACKGROUND: The modified Gompertz equation has been proposed to fit experimental data for direct current treated tumors when multiple-straight needle electrodes are individually inserted into the base perpendicular to the tumor long axis. The aim of this work is to evaluate the efficacy of direct current generated by multiple-electrode arrays on F3II mammary carcinoma that grow in the male and female BALB/c/Cenp mice, when multiple-straight needle electrodes and multiple-pairs of electrodes are inserted in the tumor. METHODS: A longitudinal and retrospective preclinical study was carried out. Male and female BALB/c/Cenp mice, the modified Gompertz equation, intensities (2, 6 and 10 mA) and exposure times (10 and 20 min) of direct current, and three geometries of multiple-electrodes (one formed by collinear electrodes and two by pair-electrodes) were used. Tumor volume and mice weight were measured. In addition, the mean tumor doubling time, tumor regression percentage, tumor growth delay, direct current overall effectiveness and mice survival were calculated. RESULTS: The greatest growth retardation, mean doubling time, regression percentage and growth delay of the primary F3II mammary carcinoma in male and female mice were observed when the geometry of multiple-pairs of electrodes was arranged in the tumor at 45, 135, 225 and 325o and the longest exposure time. In addition, highest direct current overall effectiveness (above 66%) was observed for this EChT scheme. CONCLUSIONS: It is concluded that electrochemical therapy may be potentially addressed to highly aggressive and metastic primary F3II murine mammary carcinoma and the modified Gompertz equation may be used to fit data of this direct current treated carcinoma. Additionally, electrochemical therapy effectiveness depends on the exposure time, geometry of multiple-electrodes and ratio between the direct current intensity applied and the polarization current induced in the tumor

Experimental ion mobility measurements for the LCTPC collaboration - Ar-CF4 mixtures

In this paper we present the results of the ion mobility measurements made in pure carbon tetrafluoride (CF4) and gaseous mixtures of argon with carbon tetrafluoride (Ar-CF4) for pressures ranging from 6 to 10 Torr (8-10.6 mbar) and for low reduced electric fields in the 10 Td to 25 Td range (2.4-6.1 kV center dot cm(-1)center dot bar(-1)), at room temperature. The time of arrival spectra revealed only one peak throughout the entire range studied which was attributed to CF3+. However, for Ar concentrations above 70%, a bump starts to appear at the left side of the main peak for reduced electric fields higher than 15 Td, which was attributed to impurities. The reduced mobilities obtained from the peak centroid of the time-of-arrival spectra are presented for Ar concentrations in the 5%-95% range.RD51 Collaboration/CERNFundacao para a Ciencia e a Tecnologia (FCT) - SFRH/BD/52333/201

Towards a nanospecific approach for risk assessment.

In the current paper, a new strategy for risk assessment of nanomaterials is described, which builds upon previous project outcomes and is developed within the FP7 NANoREG project. NANoREG has the aim to develop, for the long term, new testing strategies adapted to a high number of nanomaterials where many factors can affect their environmental and health impact. In the proposed risk assessment strategy, approaches for (Quantitative) Structure Activity Relationships ((Q)SARs), grouping and read-across are integrated and expanded to guide the user how to prioritise those nanomaterial applications that may lead to high risks for human health. Furthermore, those aspects of exposure, kinetics and hazard assessment that are most likely to be influenced by the nanospecific properties of the material under assessment are identified. These aspects are summarised in six elements, which play a key role in the strategy: exposure potential, dissolution, nanomaterial transformation, accumulation, genotoxicity and immunotoxicity. With the current approach it is possible to identify those situations where the use of nanospecific grouping, read-across and (Q)SAR tools is likely to become feasible in the future, and to point towards the generation of the type of data that is needed for scientific justification, which may lead to regulatory acceptance of nanospecific applications of these tools.The research leading to these results has been partially funded by the European Union Seventh Framework Programme (FP7/ 2007e2013) under the project NANoREG (A common European approach to the regulatory testing of nanomaterials), grant agreement 310584.info:eu-repo/semantics/publishedVersio

Consonance and Cantor set-selectors

It is shown that every metrizable consonant space is a Cantor set-selector. Some applications are derived from this fact, also the relationship is discussed in the framework of hyperspaces and Prohorov spaces.peer-reviewe

Chronic Hepatitis C treatment for genotype 2 or 3 in Brazil: cost effectiveness analysis of peginterferon plus ribavirin as first choice treatment

Brazilian Guidelines to HCV treatment (2007) recommended that the first choice treatment for patients with chronic hepatitis C (CHC) and genotype 2 or 3 is interferon alpha (IFN) plus ribavirin (RBV) for 24 weeks. The aim of this study is compare the cost and effectiveness to Hepatitis C treatment in patients with genotype 2 or 3 of peginterferon alpha (PEG) as the first choice of treatment within PEG for those that do not respond to IFN. The target population is CHC patients with genotype 2 or 3 in Brazil. The interventions are: PEG-SEC (first IFN plus RBV for 24 weeks, after, for non-responders and relapsers subsequently PEG plus RBV for 48 weeks); PEG-FIRST24 (PEG+RBV for 24 weeks). The type of the study is cost-effectiveness analysis. The data sources are: Effectiveness data from meta-analysis conducted on the Brazilian population. Treatment cost from Brazilian micro costing study is converted into USD (2010). The perspective is the Public Health System. The outcome measurements are Sustained Viral Response (SVR) and costs. PEG-FIRST24 (SVR: 87.8%, costs: USD 8,338.27) was more effective and more costly than PEG-SEC (SVR: 79.2%, costs: USD 5,852.99). The sensitivity analyses are: When SVR rates with IFN was less than 30% PEG-FIRST is dominant. On the other hand, when SVR with IFN was more then 75% PEG-SEC is dominant (SVR=88.2% and costs USD $ 3,753.00). PEG-SEC is also dominant when SVR to PEG24 weeks was less than 54%. In the Brazilian context, PEG-FIRST is more effective and more expensive than PEG-SEC. PEG-SEC could be dominant when rates of IFN therapy are higher than 75% or rates of PEG24 therapy are lower than 54%