Dinâmica de adubos verdes no sistema solo-planta.

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Molecular Profiling of Lymphatic Endothelial Cell Activation In Vitro

The lymphatic vascular system plays a key role in cancer progression. Indeed, the activation of lymphatic endothelial cells (LECs) through the lymphangiogenic process allows for the formation of new lymphatic vessels (LVs) that represent the major route for the dissemination of solid tumors. This process is governed by a plethora of cancer-derived and microevironmental mediators that strictly activate and control specific molecular pathways in LECs. In this work we used an in vitro model of LEC activation to trigger lymphangiogenesis using a mix of recombinant pro-lymphangiogenic factors (VFS) and a co-culture system with human melanoma cells. Both systems efficiently activated LECs, and under these experimental conditions, RNA sequencing was exploited to unveil the transcriptional profile of activated LECs. Our data demonstrate that both recombinant and tumor cell-mediated activation trigger significant molecular pathways associated with endothelial activation, morphogenesis, and cytokine-mediated signaling. In addition, this system provides information on new genes to be further investigated in the lymphangiogenesis process and open the possibility for further exploitation in other tumor contexts where lymphatic dissemination plays a relevant role

Virulência de isolados de Magnaporthe oryzae do trigo e poáceas invasoras.

Editores técnicos: Joseani Mesquita Antunes, Ana Lídia Variani Bonato, Márcia Barrocas Moreira Pimentel

Virulência de isolados de Magnaporthe oryzae do trigo e poáceas invasoras.

Orientador: João Leodato Nunes Maciel

Adubação foliar em tomateiro estaqueado. (Lycopersicum esculentum, mill). Santa Cruz - Kada.

Experimento realizado em solo latossol vermelho escuro-fase arenosa intergra de Terra roxa estruturada.Resumo

Variabilidade genética de Magnaporthe oryzae do trigo e os tipos compatíveis de populações simpátricas do patógeno.

Coorientador: João Leodato Nunes Maciel

Raças de Magnaporthe oryzae do trigo em 2013.

Editores técnicos: Joseani Mesquita Antunes, Ana Lídia Variani Bonato, Márcia Barrocas Moreira Pimentel

Cana-de-açúcar cultivada em solo adubado com lodo de esgoto: nutrientes, metais pesados e produtividade.

A pesquisa objetivou avaliar o uso de lodo de esgoto (Le) na adubação de soqueira (2o corte) de cana-de-açúcar (Saccharum spp., var. RB72-454). Aplicou-se Le ao solo, localizando-o no fundo de um sulco com 15 cm de profundidade e distando 40 cm da linha de cana. Avaliaram-se os efeitos das doses do Le (0, 15 e 30 t.ha-1) nas produtividades de biomassa e de açúcar, nos teores de nutrientes e de metais pesados do solo e da planta. O Le diminuiu a acidez potencial (H + Al) do solo e forneceu nutrientes para a cana-de-açúcar, principalmente P, S, Ca, Cu e Zn, o que refletiu em aumentos de produtividades de colmos e de açúcar por hectare. O Le causou aumentos de exportações de P, S, Ca, Cu, K, Mg e Ni pela parte aérea da cana-de-açúcar; tais aumentos, por sua vez, foram motivados pelos aumentos dos teores destes elementos no tecido vegetal, e da produtividade em biomassa. Os metais pesados (Cd, Cr, Ni e Pb), contidos no Le, não apresentaram perigo à cadeia trófica à curto prazo

Effects of benzydamine and mouthwashes containing benzydamine on Candida albicans adhesion, biofilm formation, regrowth, and persistence

Objectives To assess the effects of benzydamine and mouthwashes (MoWs) containing benzydamine on different stages of Candida albicans biofilm: adhesion, formation, persistence, and regrowth (if perturbed). Materialsandmethods C.albicansCA1398,carryingthebioluminescenceACT1p-gLUC59fusionproduct,wasemployed. Fungal cells were exposed for 1\u2032, 5\u2032, or 15\u2032 to 4 different benzydamine concentrations (0.075 to 0.6%) to 2 mouthwashes (MoWs) containing benzydamine and to a placebo MoW (without benzydamine). Treated cells were tested for adhesion (90 min) and biofilm formation (24-h assay). Next, 24- and 48-h-old biofilms were exposed to benzydamine and MoWs to assess regrowth and persistence, respectively. The effects of benzydamine, MoWs containing benzydamine, and placebo on different biofilm stages were quantified by bioluminescence assay and by the production of quorum sensing (QS) molecules. Results Benzydamine and MoWs containing benzydamine impaired C. albicans ability to adhere and form biofilm, counter- acted C. albicans persistence and regrowth, and impaired a 48-h-old biofilm. Some of these effects paralleled with alterations in QS molecule secretion. Conclusions Our results show for the first time that benzydamine and MoWs containing benzydamine impair C. albicans capacity to form biofilm and counteract biofilm persistence and regrowth. Clinical relevance Benzydamine and MoWs containing benzydamine capacity to affect C. albicans biofilm provides an interesting tool to prevent and treat oral candidiasis. Likely, restraining C. albicans colonization through daily oral hygiene may counteract colonization and persistence by other critical oral pathogens, such as Streptococcus mutans, whose increased virulence has been linked to the presence of C. albicans biofilm