Phosphorylation Provides a Negative Mode of Regulation for the Yeast Rab GTPase Sec4p

The Rab family of Ras-related GTPases are part of a complex signaling circuitry in eukaryotic cells, yet we understand little about the mechanisms that underlie Rab protein participation in such signal transduction networks, or how these networks are integrated at the physiological level. Reversible protein phosphorylation is widely used by cells as a signaling mechanism. Several phospho-Rabs have been identified, however the functional consequences of the modification appear to be diverse and need to be evaluated on an individual basis. In this study we demonstrate a role for phosphorylation as a negative regulatory event for the action of the yeast Rab GTPase Sec4p in regulating polarized growth. Our data suggest that the phosphorylation of the Rab Sec4p prevents interactions with its effector, the exocyst component Sec15p, and that the inhibition may be relieved by a PP2A phosphatase complex containing the regulatory subunit Cdc55p

Sec1p and Mso1p C-terminal tails cooperate with the SNAREs and Sec4p in polarized exocytosis

It is shown that Sec1p C-terminal tail is needed for proper Sec1p-SNARE complex interaction. Furthermore, evidence is provided that the Mso1p C terminus collaborates with the GTP-bound form of Sec4p in the bud. These results reveal a role for the Sec1p C-terminal tail in SNARE complex binding and suggest Mso1p as an effector for Sec4p

The social origins of Japanese nuclear power: a Gramscian analysis

In this paper I seek to contribute to the post-Fukushima literature on Japan, much of which accepts uncritically the orthodox narrative locating Japan’s nuclear origins in the politics of the Cold War and in the passivity of Japanese civil society vis-à-vis a ‘strong’ state. In contrast, I draw upon Gramsci’s work in order to locate these origins within wider processes of global structural transformation associated with the shift from feudalism to capitalism, and the attendant imperialism of the nineteenth century. I treat Japan’s Meiji Restoration as an instance of passive revolution within this context, one outcome of which was the adoption of a specific form of state (the ‘developmental state’) and a specific form of nationalism (techno-nationalism). I further argue that the US Occupation of Japan (1945-52) can be viewed as another instance of passive revolution. In both cases I examine the economic, political and social channels through which state goals were communicated to the Japanese populace and either embraced or resisted in turn. I suggest that Japan’s techno-nationalism survived into the post-war era, but was stripped of its overt military trappings and portrayed instead as a unique combination of ‘pacifism’ and ‘economic developmentalism’. In this way, despite being victims of nuclear weapons, ordinary Japanese people were persuaded to embrace nuclear power