miRNA-Mediated Regulation of Adult Hippocampal Neurogenesis; Implications for Epilepsy

Hippocampal neural stem/progenitor cells (NSPCs) proliferate and differentiate to generate new neurons across the life span of most mammals, including humans. This process takes place within a characteristic local microenvironment where NSPCs interact with a variety of other cell types and encounter systemic regulatory factors. Within this microenvironment, cell intrinsic gene expression programs are modulated by cell extrinsic signals through complex interactions, in many cases involving short non-coding RNA molecules, such as miRNAs. Here we review the regulation of gene expression in NSPCs by miRNAs and its possible implications for epilepsy, which has been linked to alterations in adult hippocampal neurogenesis

A Standardized Protocol for Stereotaxic Intrahippocampal Administration of Kainic Acid Combined with Electroencephalographic Seizure Monitoring in Mice

Lack of scientific reproducibility is a growing concern and weak experimental practices may contribute to irreproducibility. Here, we describe an optimized and versatile protocol for stereotaxic intrahippocampal administration of Kainic Acid (KA) in mice with a C57Bl6 background. In this protocol, KA administration is combined with in vivo recording of neuronal activity with wired and wireless setups. Following our protocol, KA administration results in a robust dose-dependent induction of low-level epileptiform activity or Status Epilepticus (SE) and induces previously characterized hallmarks of seizure-associated pathology. The procedure consists of three main steps: Craniotomy, stereotaxic administration of KA, and placement of recording electrodes in intrahippocampal, and subdural locations. This protocol offers extended possibilities compared to the systemic administration of KA, as it allows the researcher to accurately regulate the local dose of KA and resulting seizure activity, and permits the use and study of convulsive and non-convulsive KA doses, resulting in higher reproducibility and lower inter-individual variability and mortality rates. Caution should be taken when translating this procedure to different strains of mice as inter-strain sensitivity to KA has been described before. The procedure can be performed in ~1 h by a trained researcher, while intrahippocampal administration of KA without placing recording electrodes can be done in 25 min, and can be easily adapted to the titrated intrahippocampal administration of other drugs

Quarkonium in Hot Medium

I review recent progress in studying quarkonium properties in hot medium as well as possible consequences for quarkonium production in heavy ion collisions.Comment: Invited talk at SQM 2009, Buzios, Brazil, Sep. 27 -Oct. 2 2009, LaTeX, 8 pages,3 figures; typos corrected, references adde

Adult Neural Stem Cell Regulation by Small Non-coding RNAs: Physiological Significance and Pathological Implications

The adult neurogenic niches are complex multicellular systems, receiving regulatory input from a multitude of intracellular, juxtacrine, and paracrine signals and biological pathways. Within the niches, adult neural stem cells (aNSCs) generate astrocytic and neuronal progeny, with the latter predominating in physiological conditions. The new neurons generated from this neurogenic process are functionally linked to memory, cognition, and mood regulation, while much less is known about the functional contribution of aNSC-derived newborn astrocytes and adult-born oligodendrocytes. Accumulating evidence suggests that the deregulation of aNSCs and their progeny can impact, or can be impacted by, aging and several brain pathologies, including neurodevelopmental and mood disorders, neurodegenerative diseases, and also by insults, such as epileptic seizures, stroke, or traumatic brain injury. Hence, understanding the regulatory underpinnings of aNSC activation, differentiation, and fate commitment could help identify novel therapeutic avenues for a series of pathological conditions. Over the last two decades, small non-coding RNAs (sncRNAs) have emerged as key regulators of NSC fate determination in the adult neurogenic niches. In this review, we synthesize prior knowledge on how sncRNAs, such as microRNAs (miRNAs) and piwi-interacting RNAs (piRNAs), may impact NSC fate determination in the adult brain and we critically assess the functional significance of these events. We discuss the concepts that emerge from these examples and how they could be used to provide a framework for considering aNSC (de)regulation in the pathogenesis and treatment of neurological diseases

Progress in finite temperature lattice QCD

I review recent progress in finite temperature lattice calculations, including the determination of the transition temperature, equation of state, screening of static quarks and meson spectral functions.Comment: 8 pages, LaTeX, uses iopart.cls, invited talk presented at Strangeness in Quark Matter 2007 (SQM 2007), Levoca, Slovakia, June 24-29, 200

Concerning the quark condensate

A continuum expression for the trace of the massive dressed-quark propagator is used to explicate a connection between the infrared limit of the QCD Dirac operator's spectrum and the quark condensate appearing in the operator product expansion, and the connection is verified via comparison with a lattice-QCD simulation. The pseudoscalar vacuum polarisation provides a good approximation to the condensate over a larger range of current-quark masses.Comment: 7 pages, LaTeX2e, revtex

Spectrum of the U(1) staggered Dirac operator in four dimensions

We compare the low-lying spectrum of the staggered Dirac operator in the confining phase of compact U(1) gauge theory on the lattice to predictions of chiral random matrix theory. The small eigenvalues contribute to the chiral condensate similar as for the SU(2) and SU(3) gauge groups. Agreement with the chiral unitary ensemble is observed below the Thouless energy, which is extracted from the data and found to scale with the lattice size according to theoretical predictions.Comment: 5 pages, 3 figure