In Vitro Preparation And Testing Of Anti-Salmonella Vaccine Against Abortion In Sheep

Abstract: In March 2016, the microbiology laboratory of the Faculty of Veterinary Medicine in Cluj-Napoca, 3 type of antisalmonella vaccine for sheep were prepared. For type 1, 24 hours salmonella on brain heart infusion (BHI) broth culture, heat inactivated for 1 hour at 60°C, then with formaldehyde in a concentration of 3 ‰. Variant 2 - the culture supernatant obtained on solid BHI medium, washed with PBS, frozen-thawed 6 times, centrifuged at 4000 rpm for 15 minutes, filtered through 0.2 μm Millipore filter Orange Scientific. Variant 3 - suspension of cell walls remaining after centrifugation suspended in PBS and inactivated with formaldehyde.After the bacterial and fungal sterility control, the three types of vaccine were administered in rabbits by the subcutaneous route at a dose of 1 ml/individual along with 1 ml of plant extract adjuvant (decoction of Ewernia prunastri) sterilized by filtration.There have been two booster inoculations of the initial administration, 7 and 14 days after. Before each dose of vaccine, blood was sampled from the marginal auricular vein in order to control the immunogenicity by anti-somatic ˮOˮ serum antibody (Ab) titration using slow microplate agglutination test (Widal reaction). After three inoculations with the vaccine variant 1, Ab serum titer reached 1/128, and in types 2 and 3, 1/512 after 2 inoculations, decreasing to 1/256 after the second booster administered with no immunomodulator

Advances in Additive Manufacturing and Their Applications

Additive manufacturing (AM) has evolved rapidly in the last few years [...

Silver Nanoparticles (AgNP) impregnated filters in drinking water disinfection

This paper describes how simple portable devices could eliminate water pathogens by using Silver Nanoparticles, based on their antimicrobial properties. Recent studies indicated that silver nanoparticles can achieve up to 100% antibacterial activity removal. Results are showing that Silver Nanoparticles retention in the filter structure, E. coli bacteria removal, water quality and water flow rate must be evaluated as main efficiency indicators of the designed filters, in order to obtain the optimal filter. To apply the antimicrobial property of Silver in drinking-water treatment, a filter is produced using Additive Manufacturing techniques and coated with different concentrations of silver solutions

Silver Nanoparticles (AgNP) impregnated filters in drinking water disinfection

This paper describes how simple portable devices could eliminate water pathogens by using Silver Nanoparticles, based on their antimicrobial properties. Recent studies indicated that silver nanoparticles can achieve up to 100% antibacterial activity removal. Results are showing that Silver Nanoparticles retention in the filter structure, E. coli bacteria removal, water quality and water flow rate must be evaluated as main efficiency indicators of the designed filters, in order to obtain the optimal filter. To apply the antimicrobial property of Silver in drinking-water treatment, a filter is produced using Additive Manufacturing techniques and coated with different concentrations of silver solutions

Bending and compression tests for PA 2200 parts obtained using Selective Laser Sintering method

Craniofacial bone defects that produce morphological and functional disabilities can bring alteration of the quality of patient’s life. For this reason, the collaboration between the field of medicine with engineering, lead to the manufacture of custom implants from biocompatible materials. In the last years it was shown that Additive Manufacturing methods are very helpful to achieve customized medical implants. All the customized implants made of biocompatible materials have to be tested for mechanical properties, in order to have the optimal characteristics for the area were will be used. This paper presents the results for bending and compression tests for a biocompatible material, PA 2200. The samples were achieved using Selective Laser Sintering method and were obtained with different laser power, starting with 4 W. Maximum load, maximum stress, specific deformation and Young's modulus were analysed. The study showed that different mechanical properties can be obtained, depending on the laser power used. At bending tests, increasing values were obtained for the investigated parameters along with laser power increasing, but at compression tests a different trend was observed

Research Regarding the Manufacturing through AM Technologies of an Implant for Cervical Disc Replacement

Worldwide, accidents are one of the main causes of illness in developed and undeveloped socio-economic countries. Additive Manufacturing (AM) technologies bring extremely useful and advantageous applications for the new neurosurgical procedures. Because the surgical insertion of devices for cervical disc replacement is very difficult, the development of new devices that can minimize these disadvantages, are needed. The aim of this paper was to improve the characteristics of an implant for cervical intervertebral disc replacement, a model based on an existing implant. A cervical cage designed for stabilization and arthrodesis between the cervical vertebrae was analyzed. A new design of a cage that have some improvements, useful for patient safety, as well as for facilitating the surgery was developed. The new proposed design was verified through Fine Element Analysis

Research Regarding the Manufacturing through AM Technologies of an Implant for Cervical Disc Replacement

Worldwide, accidents are one of the main causes of illness in developed and undeveloped socio-economic countries. Additive Manufacturing (AM) technologies bring extremely useful and advantageous applications for the new neurosurgical procedures. Because the surgical insertion of devices for cervical disc replacement is very difficult, the development of new devices that can minimize these disadvantages, are needed. The aim of this paper was to improve the characteristics of an implant for cervical intervertebral disc replacement, a model based on an existing implant. A cervical cage designed for stabilization and arthrodesis between the cervical vertebrae was analyzed. A new design of a cage that have some improvements, useful for patient safety, as well as for facilitating the surgery was developed. The new proposed design was verified through Fine Element Analysis