Gastroprotective effects of oral nucleotide administration

BACKGROUND AND AIMS: Nucleotides form the building blocks of DNA and are marketed as dietary supplements, alone or in combination with other ingredients, to promote general health. However, there has been only limited scientific study regarding the true biological activity of orally administered nucleotides. We therefore tested their efficacy in a variety of models of epithelial injury and repair. METHODS: Effects on proliferation ([(3)H] thymidine incorporation) and restitution (cell migration of wounded monolayers) were analysed using HT29 and IEC6 cells. The ability of a nucleotide mixture to influence gastric injury when administered orally and subcutaneously was analysed using a rat indomethacin (20 mg/kg) restraint model. RESULTS: In both cell lines, cell migration was increased by approximately twofold when added at 1 mg/ml (p<0.01); synergistic responses were seen when a mixture of nucleotides was used. Cell proliferation was stimulated by adenosine monophosphate (AMP) in HT29, but not in IEC6, cells. Gastric injury was reduced by approximately 60% when gavaged at 4–16 mg/ml (p<0.05), concentrations similar to those likely to be found in consumers taking nucleotide supplements. Systemic administration of nucleotides was unhelpful. CONCLUSIONS: Nucleotides possess biological activity when analysed in a variety of models of injury and repair and could provide a novel inexpensive approach for the prevention and treatment of the injurious effects of non steroidal anti‐inflammatory drugs and other ulcerative conditions of the bowel. Further studies on their potential benefits (and risks) appear justified

Dois Casos de Quérion por Trichophyton Mentagrophytes

Introdução: O Quérion é uma forma rara e infl amatória de tinea capitis causada por fungos dermatófi tos. O diagnóstico é difi cultado pela demora no exame cultural e possibilidade de várias etiologias no diagnóstico diferencial. O tratamento é prolongado e implica o recurso a antifúngicos sistémicos. Novos fármacos têm sido considerados após a descontinuação da griseofulvina em Portugal. Casos clínicos: Apresentamos duas crianças com lesões dolorosas do couro cabeludo com reação infl amatória exuberante, exsudativa e alopécia local acompanhadas de febre. O exame bacteriológico foi negativo e o micológico revelou Trichophyton mentagrophytes. Foram medicados com fl ucloxacilina endovenosa, antifúngicos orais (griseofulvina e itraconazol), corticoide e antifúngico tópicos. A resposta foi favorável com evolução lenta para a cura com área de alopécia residual. Conclusão: Estes casos destacam -se pela sua raridade e ilustram a exuberância das manifestações clínicas desta entidade. O diagnóstico precoce, a identificação e tratamento da fonte de contágio e as medidas de higiene e vigilância sanitária são primordiais para o controlo da infecção

CONTROLO NATURAL DO PARASITISMO GASTROINTESTINAL EM CAPRINOS E EFEITO NA CONTAMINAÇÃO AMBIENTAL

Na perspetiva de um controlo integrado, é importante o estudo de métodos alternativos ao uso exclusivo de anti‑helmínticos sintéticos, e privilegiar tratamentos seletivos

Doentes Oncológicos com Síndrome Coronária Aguda Não Têm Maior Risco Hemorrágico Quando Comparados com Doentes com Características Similares - Uma Análise de Emparelhamento de Score de Propensão do Registo ProACS

Introduction: The management of acute coronary syndrome (ACS) in malignancy is challenging due to higher bleeding risk. Methods: We analyzed patients with cancer (active or in the previous five years) prospectively included in the ProACS registry between 2010 and 2019. Our aim was to assess safety (major bleeding, primary endpoint) and secondary efficacy endpoints (in-hospital mortality and combined in-hospital mortality, reinfarction and ischemic stroke) of ACS treatment. Propensity score matching analysis (1:1) was further performed to better understand predictors of outcomes. Results: We found 934 (5%) cancer patients out of a total of 18 845 patients with ACS. Cancer patients had more events: major bleeding (2.9% vs. 1.5%), in-hospital mortality (5.8% vs. 3.4%) and the combined endpoint (7.4% vs. 4.9%). The primary endpoint was related to cancer diagnosis (OR 1.97), previous bleeding (OR 7.09), hemoglobin level (OR 4.94), atrial fibrillation (OR 3.50), oral anticoagulation (OR 3.67) and renal dysfunction. Mortality and the combined secondary endpoint were associated with lower use of invasive coronary angiography and antiplatelet and neurohormonal blocker therapy. After propensity score matching (350 patients), there were no statistically significant differences in endpoints between the populations. Conclusion: Bleeding risk was not significant higher in the cancer population compared to patients with similar characteristics, nor were mortality or ischemic risk. The presence of cancer should not preclude simultaneous ACS treatment.info:eu-repo/semantics/publishedVersio

A Comparative Proteomic Analysis of Praziquantel-Susceptible and Praziquantel-Resistant Schistosoma mansoni Reveals Distinct Response Between Male and Female Animals

publishersversionpublishe

Parasitologicalassessment and host-parasite relationship in farmed Cachara catfish fingerlings (Pseudoplatystoma reticulatum Eigenmann & Eigenmann 1889), Mato Grosso do Sul, Brazil.

Farmed fingerlings of South American catfish are frequently exposed to several parasites, but little information is available for implementing health programs for control and prevention of parasitic diseases in the hatcheries. This study evaluated the parasitic fauna of cachara catfish fingerlings (Pseudoplatystoma reticulatum Eingenmann & Eigenmann, 1889) and the hostparasite relationship during the early stages of rearing. A total of 302 cachara fingerlings were used for parasitic diagnosis and histopathological analysis. Ichthyophthirius multifiliis and Trichodina heterodentata were diagnosed as the most prevalent parasites, followed by Cryptobia sp., Henneguya sp., Monogenea and Nematoda. There was a positive correlation between the size of the fish and the mean intensity of parasitism by I. multifiliis. Proliferation of mucus cells, club cells, multifocal area of degeneration, epithelial necrosis on the body surface, skin ulceration, fusion of secondary gill lamellae and inflammatory infiltration were observed in I. multifiliisinfected fish. Ciliated protozoans were the main etiological agents diagnosed, and the cachara (P. reticulatum) is a new host for T. heterodentata. In addition, ichthyophthiriasis induced severe tissue damage thus making the fingerlings susceptible to opportunistic infections.Los alevines de bagres del Sur de América en cautiverio están frecuentemente expuestos a muchos parásitos, pero poca información es evaluada para implementar programa de salud para control y prevención de enfermedades en criaderos. Este estudio evaluó la fauna de parásitos de alevines de cachara (Pseudoplatystoma reticulatum Eingenmann & Eingenmann, 1889) y la relación huésped-parásito durante las fases iníciales de cultivo. Un total de 302 alevines fueran sometidos a diagnóstico y análisis histopatológicos. Ichthyophthirius multifiliis y Trichodina heterodentata fueran diagnosticados como los parásitos mas prevalentes, seguidos de Cryptobia sp., Henneguya sp., Monogenea y Nematoda. Ha sido observada una correlación positiva entre la longitud de los peces y la intensidad promedio de I. multifiliis. Proliferación de células de moco, células club, áreas multifocales de degeneración, necrosis epitelial en la superficie del cuerpo, ulceración de piel, fusión de laminillas branquiales secundarias e infiltración inflamatoria fueron observados en peces infectados por I. multifiliis. Los protozoarios ciliados fueran los agentes etiológicos más importantes y el cachara (P. reticulatum) es un nuevo huésped para T. heterodentata. En adición, la ictioftiriasis indujo un daño severo favoreciendo las infecciones oportunistas en los alevines

Yarrowia lipolytica growth under increased air pressure: influence on enzymes production

Improvement of microbial cell cultures oxygenation can be achieved by the increase of total air pressure, which increases oxygen solubility in the medium. In this work, a pressurized bioreactor was used for Yarrowia lipolytica batch cultivation under increased air pressure from 1 to 6 bar. Cell growth was strongly enhanced by the pressure rise. Fivefold and 3.4-fold increases in the biomass production and in specific growth rate, respectively, were observed under 6 bar. The increase of oxygen availability caused the induction of the antioxidant enzyme superoxide dismutase, which indicates that the defensive mechanisms of the cells against oxidative stress were effective and cells could cope with increased pressure. The pregrowth of Y. lipolytica under increased pressure conditions did not affect the lipase production ability of the cells. Moreover, the extracellular lipase activity increased 96% using a 5-bar air pressure instead of air at 1- bar pressure during the enzyme production phase. Thus, air pressure increase in bioreactors is an effective mean of cell mass and enzyme productivity enhancement in bioprocess based in Y. lipolytica cultures

Observation of confined current ribbon in JET plasmas

we report the identification of a localised current structure inside the JET plasma. It is a field aligned closed helical ribbon, carrying current in the same direction as the background current profile (co-current), rotating toroidally with the ion velocity (co-rotating). It appears to be located at a flat spot in the plasma pressure profile, at the top of the pedestal. The structure appears spontaneously in low density, high rotation plasmas, and can last up to 1.4 s, a time comparable to a local resistive time. It considerably delays the appearance of the first ELM.Comment: 10 pages, 6 figure