Rickettsial pathogens and their arthropod vectors.

Rickettsial diseases, important causes of illness and death worldwide, exist primarily in endemic and enzootic foci that occasionally give rise to sporadic or seasonal outbreaks. Rickettsial pathogens are highly specialized for obligate intracellular survival in both the vertebrate host and the invertebrate vector. While studies often focus primarily on the vertebrate host, the arthropod vector is often more important in the natural maintenance of the pathogen. Consequently, coevolution of rickettsiae with arthropods is responsible for many features of the host-pathogen relationship that are unique among arthropod-borne diseases, including efficient pathogen replication, long-term maintenance of infection, and transstadial and transovarial transmission. This article examines the common features of the host-pathogen relationship and of the arthropod vectors of the typhus and spotted fever group rickettsiae

Muon spin rotation studies of niobium for superconducting RF applications

In this work we investigate superconducting properties of niobium samples via application of the muon spin rotation/relaxation (muSR) technique. We employ for the first time the muSR technique to study samples that are cutout from large and small grain 1.5 GHz radio frequency (RF) single cell niobium cavities. The RF test of these cavities was accompanied by full temperature mapping to characterize the RF losses in each of the samples. Results of the muSR measurements show that standard cavity surface treatments like mild baking and buffered chemical polishing (BCP) performed on the studied samples affect their surface pinning strength. We find an interesting correlation between high field RF losses and field dependence of the sample magnetic volume fraction measured via muSR. The muSR line width observed in ZF-muSR measurements matches the behavior of Nb samples doped with minute amounts of Ta or N impurities. An upper bound for the upper critical field Hc2 of these cutouts is found.Comment: 20 pages, 14 figure

Application of an Ultralight Aircraft to Aerial Surveys of Kangaroos on Grazing Properties

A Drifter ultralight aircraft was used as a platform for line-transect aerial surveys of three species of kangaroo in the sheep rangelands south-east of Blackall and north of Longreach in central-western Queensland in winter 1993 and 1994. Favourable comparisons between the results of ultralight surveys and those made from a helicopter flying the same transects and foot surveys along another set of transects, all within a few days of the ultralight survey, confirmed the expectation we had that an ultralight would be a satisfactory and much cheaper vehicle for conducting aerial surveys of kangaroos. The comparisons are even more favourable when data for the three species surveyed are combined, pointing to a problem in species identification and underlining the importance of using only experienced observers for aerial survey of kangaroos, whatever the platform. The use of an ultralight aircraft could have particular value where a comparatively small area, such as an individual sheep or cattle property, is under consideration. In this paper, we present the numerical comparisons, along with an evaluation of the practicability of using this type of aircraft. We also describe a possible future scenario in which an accreditation process could see approved kangaroo surveyors undertaking property assessments by ultralight, under contract to graziers or other interested parties

Shelter selection and home range of echidnas, tachyglossus aculeatus, in the highlands of South-East Queensland

We recorded physical attributes of sites used for daily shelter or as hibernacula by nine echidnas, Tachyglossus aculeatus, living on a sheep grazing property in southeastern Queensland. Home ranges were also determined. For daily shelter sites, echidnas most often used hollow logs and depressions under the roots of fallen trees. Daily shelters were found more often on north-facing slopes and usually provided >90% cover. Hibernation sites always provided 100% cover but, in contrast to daily sites, were distributed randomly in relation to the slope of the terrain. Rabbit burrows, which offer much better thermal buffering than hollow logs, were used most commonly as hibernacula. There were differences between individuals in the type of shelter they used and, moreover, echidnas did not use shelter sites in proportion to what was available, indicating that choice was being exercised. Re-use of sites occurred non-randomly, implying that echidnas can recognise previously used shelters and probably have a learned familiarity with their living area. Home ranges varied between 20.6 ha and 93.3 ha (mean = 49.8, s.d. = 25.4), overlapped with those of other echidnas and correlated positively with body weight

Crocodiles as dinosaurs: Behavioural thermoregulation in very large ectotherms leads to high and stable body temperatures

Empirical field data describing daily and seasonal cycles in body temperature (Tb) of free-ranging Crocodylus porosus (32-1010 kg) can be predicted by a mathematical analysis. The analysis provides a mechanistic explanation for the decreased amplitude of daily cycles in Tb and the increase in 'average' Tb with increasing mass. Assessments of 'average' daily Tb were made by dividing the integral of the difference between measured values of Tb and minimum operative temperature by the period of integration, to yield a thermal index expressing relative 'warmth' of crocodiles. The average daily Tb of a 1010 kg crocodile was 3.7 degrees C warmer than that of a 42 kg individual in summer and 1.9 degreesC warmer than that of a 32 kg individual in winter. The success of this mathematical approach confirms that crocodiles are simple ectotherms and that there is unlikely to be a significant contribution to their thermal biology from physiological mechanisms. Behaviour, however, is very important even in large individuals. Crocodiles in the field typically move daily between land and water in cycles that vary seasonally. We predicted Tb for the reverse of these behavioural cycles, which more than doubled seasonal fluctuations in Tb compared with the observed fluctuations. We were also able to predict the Tb of very large, dinosaur-sized crocodiles in a similar climate to that at our study site. A 10 000 kg 'crocodile', for example, would be expected to have a Tb of 31 degreesC in winter, varying by less than 0.1 degrees C during a day when operative temperatures varied by nearly 20 degrees C, from 20 to 38 degrees C. The study confirms that, in low latitudes at least, large dinosaurs must have had an essentially high and stable value of Tb, without any need for endothermy. Also, access to shade or water must have been crucial for the survival of large dinosaurs at low latitudes. Furthermore, the finding of increasing 'average' Tb as ectotherms grow larger may have implications for the metabolic rates of very large reptiles, because the Q10 effect could counteract the downscaling of metabolic rate with mass, an effect that seems not to have been recognised previously

The Evolution of Endothermy and Its Diversity in Mammals and Birds

Many elements of mammalian and avian thermoregulatory mechanisms are present in reptiles and the changes involved in the transition to endothermy are more quantitative than qualitative. Drawing on our experience with reptiles and echidnas, we comment on that transition and on current theories about how it occurred. The theories divide into two categories, depending upon whether selection pressures operated directly or indirectly on mechanisms producing heat. Both categories of theories focus on explaining the evolution of homeothermic endothermy but ignore heterothermy. However, noting that hibernation and torpor are almost certainly plesiomorphic (= ancestral, primitive), and that heterothermy is very common among endotherms, we propose that homeothermic endothermy evolved via heterothermy, with the earliest protoendotherms being facultatively endothermic and retaining their ectothermic capacity for "constitutional eurythermy". Thus, unlike current models for the evolution of endothermy which assume that hibernation and torpor are specialisations arising from homeothermic ancestry, and therefore irrelevant, we consider that they are central. We note the sophistication of thermoregulatory behavior and control in reptiles, including precise control over conductance, and argue that brooding endothermy seen in some otherwise ectothermic Boidae suggests an incipient capacity for facultative endothermy in reptiles. We suggest that the earliest insulation in protoendotherms may have been internal, arising from of re-distribution of the fat bodies which are typical of reptiles. We note that short-beaked echidnas provide a useful living model of what an (advanced) protoendotherm may have been like. Echdinas have the advantages of endothermy, including the capacity for homeothermic endothermy during incubation, but are very relaxed in their thermoregulatory precision and minimise energetic costs by using ectothermy facultatively when entering short or long term torpor. They also have a substantial layer of internal dorsal insulation. We favor theories about the evolution of endothermy which invoke direct selection for the benefits conferred by warmth, such as expanding daily activity into the night, higher capacities for sustained activity, higher digestion rates, climatic range expansion and, not unrelated, control over incubation temperature and the benefits for parental care. We present an indicative, stepwise schema in which observed patterns of body temperature are a consequence of selection pressures, the underlying mechanisms and energy optimization, and in which homeothermy results when it is energetically desirable, rather than as the logical endpoint

Ecological niche modeling and differentiation of populations of *Triatoma brasiliensis* Neiva, 1911, the most important Chagas disease vector in northeastern Brazil (Hemiptera, Reduviidae, Triatominae)

Am. J. Trop. Med. Hyg., 67(5), 2002, pp. 516–520 Copyright © 2002 by The American Society of TropicalMedicine and Hygiene 516 http://www.ajtmh.org/cgi/reprint/67/5/516Ecologic niche modeling has allowed numerous advances in understanding the geographic ecology of species, including distributionalpredictions, distributionalchange and invasion, and assessment of ecologic differences. We used this toolto characterize ecologic differentiation of Triatoma brasiliensis populations, the most important Chagas’ disease vector in northeastern Brazil. The species’ ecologic niche was modeled based on data from the Fundação Nacionalde Saúde of Brazil(1997–1999) with the Genetic Algorithm for Rule-Set Prediction (GARP). This method involves a machine-learning approach to detecting associations between occurrence points and ecologic characteristics of regions. Four independent “ecologic niche models” were developed and used to test for ecologic differences among T. brasiliensis populations. These models confirmed four ecologically distinct and differentiated populations, and allowed characterization of dimensions of niche differentiation. Patterns of ecologic similarity matched patterns of molecular differentiation, suggesting that T. brasiliensis is a complex of distinct populations at various points in the process of speciation

Molecular Identification of Eimeria Species in Broiler Chickens in Trinidad, West Indies

Coccidiosis is an intestinal disease of chickens of major economic importance to broiler industries worldwide. Species of coccidia found in chickens include Eimeria acervulina, Eimeria brunetti, Eimeria maxima, Eimeria mitis, Eimeria necatrix, Eimeria praecox, and Eimeria tenella. In recent years, polymerase chain reaction (PCR) has been developed to provide accurate and rapid identification of the seven known Eimeria species of chickens. The aim of this study was to use species-specific real-time PCR (qPCR) to identify which of the seven Eimeria species are present in Trinidad poultry. Seventeen pooled fecal samples were collected from 6 broiler farms (2–5 pens per farm) across Trinidad. Feces were also collected from birds showing clinical signs of coccidiosis in two live bird markets (pluck shops). qPCR revealed the presence of five species of Eimeria (E. acervulina, E. maxima, E. mitis, E. necatrix, and E. tenella), but not E. brunetti or E. praecox. Mixed infections were detected on all broiler farms, and DNA of two highly pathogenic Eimeria species (E. tenella and E. necatrix) was detected in feces taken from clinically sick birds sampled from the two pluck shops