The Neolithic greenstone industry of Chiomonte (northwestern Italy): mineralogy, petrography and archaeometric implications

Abstract. The polished stone industry of Chiomonte (Piedmont region, northwestern Italy), dating back to the middle to late Neolithic, has been studied with a multi-analytical approach, including mineralogical, petrographic and morpho-typological issues, with the aim of providing information about the sources of the raw materials and determining the function of this particular settlement in the prehistoric Western Alps. Most of the lithic tools are made of sensu stricto greenstones (i.e. "Na pyroxene rocks" and "Na pyroxene and garnet rocks"), though a large number of serpentinite tools (25 %) also exist. The combined application of X-ray powder diffraction (XRPD), polarising microscopy and Scanning Electron Microscopy coupled with Energy Dispersive Spectrometry (SEM-EDS) led to the detection of specific mineral and chemical "markers", pointing to the Chiomonte tools likely having come from the Monviso area. However, other closer supply sources, e.g. small meta-ophiolite units in the Orsiera–Rocciavré mountain range or in the lower Susa valley, cannot be ruled out. The presence, on the many retrieved roughouts and broken tools, of raw, yet unpolished surfaces that are ascribable to pebbles and cobbles from alluvial or glacial deposits, suggests that these rocks had been picked up from local "secondary" sources. The abundance of roughouts and broken tools identifies Chiomonte as a second-order manufacturing site, although it is still unclear whether such an activity was restricted to serving local needs or if it contributed to the circulation of greenstone implements on a wider scale

The association of micronucleus frequency with obesity, diabetes and cardiovascular disease

Obesity and metabolic syndrome (MetS) are serious and growing health care problems worldwide, leading an increased risk for type 2 diabetes (T2D) and cardiovascular disease (CVD). Over the past decade, emerging evidence has shown that an increased chromosomal 20 damage, as determined by the cytokinesis-block micronucleus (CBMN) assay, is correlated to the pathogenesis of metabolic and CVD. An increased micronuclei (MN) frequency has been demonstrated in peripheral blood lymphocytes of patients with polycystic ovary syndrome, 25 a common condition in reproductive-aged women associated with impaired glucose tolerance, T2D mellitus and the MetS. High levels of MN have been detected to be significantly correlated with T2D as well as with the occurrence and the severity of coronary artery disease 30 (CAD). Long-term follow-up studies have shown that an increased MN frequency is a predictive biomarker of cardiovascular mortality within a population of healthy subjects as well as of major adverse cardiovascular events in patients with known CAD. Overall, these findings 35 support the hypothesis that CBMN assay may provide an useful tool for screening of the MetS and its progression to diabetes and CVD in adults as well in children. Large population-based cohorts are needed in order to compare the MN frequencies as well as to better define whether MN 40 is a biomarker or a mediator of cardiometabolic diseases

Elevated urine levels of bufotenine in patients with autistic spectrum disorders and schizophrenia

Previous studies have suggested that the endogeneous psychotomimetic molecule bufotenine (N-N notdimethyl-5-idroxytryptamine) may play a role in the pathogenesis of severe mental disorders. The potential association of bufotenine with the clinical features of autism and schizophrenia is not entirely understood. In this study, we measured urinary levels of bufotenine in subjects with autistic spectrum disorder (ASD), schizophrenia and healthy comparison subjects free of psychiatric symptoms. We also sought to assess whether urine concentrations of this molecule may be associated with the clinical characteristics of psychiatric patients. DESIGN: Urine bufotenine levels were measured using a high-performance liquid chromatography-mass spectrometry (HPLC-MS) assay in young adults with severe ASD (n = 15), patients with schizophrenia (n = 15), and healthy control subjects (n = 18). The Vineland Adaptive Behavior Scale was used to measure adaptive behaviors in ASD individuals. The Brief Psychiatric Rating Scale (BPRS) was used for patients with schizophrenia. RESULTS: Urine bufotenine levels were significantly higher in ASD subjects (3.30 +/- 0.49 mug/L, P < 0.05) and patients with schizophrenia (4.39 +/- 0.43 mug/L, P < 0.001) compared with controls (1.53 +/- 0.30 mug/L). Among patients with ASD, there was a significant positive correlation between urine bufotenine and hyperactivity scores on the Vineland Adaptive Behavior Scale (r = 0.479, P < 0.05). No other associations were detected. CONCLUSIONS: our results indicate that elevated urine levels of the endogeneous psychotomimetic molecule bufotenine may play a role in ASD and schizophrenia, and can be correlated with hyperactivity scores in autism

Bitter taste receptor polymorphisms and human aging.

Several studies have shown that genetic factors account for 25% of the variation in human life span. On the basis of published molecular, genetic and epidemiological data, we hypothesized that genetic polymorphisms of taste receptors, which modulate food preferences but are also expressed in a number of organs and regulate food absorption processing and metabolism, could modulate the aging process. Using a tagging approach, we investigated the possible associations between longevity and the common genetic variation at the three bitter taste receptor gene clusters on chromosomes 5, 7 and 12 in a population of 941 individuals ranging in age from 20 to 106 years from the South of Italy. We found that one polymorphism, rs978739, situated 212 bp upstream of the TAS2R16 gene, shows a statistically significant association (p = 0.001) with longevity. In particular, the frequency of A/A homozygotes increases gradually from 35% in subjects aged 20 to 70 up to 55% in centenarians. These data provide suggestive evidence on the possible correlation between human longevity and taste genetics