Atención Farmacéutica, transversalidad en la Facultad de Farmacia de la Universidad de Costa Rica

After a long evolving process, Pharmaceutical Care has become the transversal axis of the curriculum of the Schoolof Pharmacy of the University of Costa Rica, who has redefi ned its focus of study, as well as the pedagogic approachof the curricular work. With this transversality, the patient becomes the center in which all the knowledge, attitudes,aptitudes and skills promoted in the students, convey. With this, it is pretended to develop a better pharmacist who canmore properly fi nd his place in modern society.Después de un largo proceso evolutivo, la Atención Farmacéutica se ha convertido en el eje transversal del plan deestudios de la Facultad de Farmacia de la Universidad de Costa Rica, quien ha redefi nido su objeto de estudio asícomo el abordaje pedagógico del quehacer curricular. Con esta transversalidad, el paciente se convierte en el centro enel que convergen todos los conocimientos, actitudes, aptitudes y destrezas que se promueven en el estudiante. Con estose pretende producir un mejor farmacéutico que encuentre más adecuadamente su sitio en la sociedad actual

Flower consumption, ambient temperature and rainfall modulate drinking behavior in a folivorous-frugivorous arboreal mammal

Water is vital for the survival of any species because of its key role in most physiological processes. However, little is known about the non-food-related water sources exploited by arboreal mammals, the seasonality of their drinking behavior and its potential drivers, including diet composition, temperature, and rainfall. We investigated this subject in 14 wild groups of brown howler monkeys (Alouatta guariba clamitans) inhabiting small, medium, and large Atlantic Forest fragments in southern Brazil. We found a wide variation in the mean rate of drinking among groups (range = 0–16 records/day). Streams (44% of 1,258 records) and treeholes (26%) were the major types of water sources, followed by bromeliads in the canopy (16%), pools (11%), and rivers (3%). The type of source influenced whether howlers used a hand to access the water or not. Drinking tended to be evenly distributed throughout the year, except for a slightly lower number of records in the spring than in the other seasons, but it was unevenly distributed during the day. It increased in the afternoon in all groups, particularly during temperature peaks around 15:00 and 17:00. We found via generalized linear mixed modelling that the daily frequency of drinking was mainly influenced negatively by flower consumption and positively by weekly rainfall and ambient temperature, whereas fragment size and the consumption of fruit and leaves played negligible roles. Overall, we confirm the importance of preformed water in flowers to satisfy the howler’s water needs, whereas the influence of the climatic variables is compatible with the ‘thermoregulation/dehydration-avoiding hypothesis’. In sum, we found that irrespective of habitat characteristics, brown howlers seem to seek a positive water balance by complementing the water present in the diet with drinking water, even when it is associated with a high predation risk in terrestrial sources.Coordinación de la formación del personal de nivel superior/[2755/2010]/CAPES/BrasilNational Council for scientific and Technological Development/[303306/2013-0]/CNPq/BrasilNational Council for scientific and Technological Development/[304475/2018-1]/CNPq/BrasilNational Council for scientific and Technological Development/[140641/2016-5]/CNPq/BrasilUCR::Vicerrectoría de Docencia::Ciencias Básicas::Facultad de Ciencias::Escuela de Biologí

Altered thiol chemistry in human amyotrophic lateral sclerosis-linked mutants of superoxide dismutase 1

Neurodegenerative diseases share a common characteristic, the presence of intracellular or extracellular deposits of protein aggregates in nervous tissues. Amyotrophic Lateral Sclerosis (ALS) is a severe and fatal neurodegenerative disorder, which affects preferentially motoneurons. Changes in the redox state of superoxide dismutase 1 (SOD1) are associated with the onset and development of familial forms of ALS. In human SOD1 (hSOD1), a conserved disulfide bond and two free cysteine residues can engage in anomalous thiol/disulfide exchange resulting in non-native disulfides, a hallmark of ALS that is related to protein misfolding and aggregation. Because of the many competing reaction pathways, traditional bulk techniques fall short at quantifying individual thiol/disulfide exchange reactions. Here, we adapt recently developed single-bond chemistry techniques to study individual disulfide isomerization reactions in hSOD1. Mechanical unfolding of hSOD1 leads to the formation of a polypeptide loop held by the disulfide. This loop behaves as a molecular jump rope that brings reactive Cys-111 close to the disulfide. Using force-clamp spectroscopy, we monitor nucleophilic attack of Cys-111 at either sulfur of the disulfide and determine the selectivity of the reaction. Disease-causing mutations G93A and A4V show greatly altered reactivity patterns, which may contribute to the progression of familial ALS

Initiating activation in diabetes self-management from patients’ perspective

Patients’ active role is crucial in managing chronic illnesses like type 2 diabetes. Current evidence demonstrated patients who were activated had better clinical outcomes. Some factors influencing patient activation have been identified but there is little knowledge on how patients successfully start to take their role in managing diabetes. This study aimed to explore how patients begin to activate themselves in self-management. The narrative inquiry was taken as the qualitative methodological approach. Ten participants with type 2 diabetes were purposively sampled from a public primary care clinic in Malaysia. In-depth interviews were conducted and recorded. The data were analysed with thematic analysis. Three themes and four subthemes were generated. The first theme, “Learning what to do” describes the learning process from social networks and healthcare professionals that helped to define their role. The second theme highlighted that patients wished for a gradual approach in initiating activation, i.e. “Taking one step at a time”. They started with a more straightforward self-management task and needed more time to take up their role. The third theme pointed out that when patients could not perform certain self-management tasks, they would engage with someone else to help: “Engaging with someone for challenging tasks.” In conclusion, diabetes self-management education is substantial in developing ‘patient activation,’ i.e. patient’s ability to self-manage. The self-management plan should also be realistic and suit patients’ circumstances. Having a health partner or buddy among family members or peers is helpful when trying to adopt good dietary habits and perform regular exercise

Sugarcane stem borers of the Colombian Cauca River Valley: current pest status, biology, and control

Citation: Vargas, G., Gomez, L. A., & Michaud, J. P. (2015). Sugarcane stem borers of the Colombian Cauca River Valley: current pest status, biology, and control. Florida Entomologist, 98(2), 728-735. Retrieved from ://WOS:000356451400049Sugarcane stem borers of the genus of Diatraea (Lepidoptera: Crambidae) form a species complex that causes serious economic losses to sugarcane production in the Cauca River Valley and other regions of Colombia. Two primary species, Diatraea saccharalis (F.) and D. indigenella Dyar and Heinrich, have been effectively managed for more than 4 decades through augmentative releases of the tachinid flies Lydella minense (Townsend) and Billaea claripalpis (Wulp) (Diptera: Tachinidae) and the egg parasitoid Trichogramma exiguum Pinto & Platner (Hymenoptera: Trichogrammatidae). Here we review the current pest status of Diatraea species, damage assessment protocols, management tactics, and the environmental factors and cultural practices that can affect biological control outcomes. Recent changes in the cultivars grown have the potential to increase pest populations and diminish biological control efficacy. Additionally, recent outbreaks of new Diatraea species may further increase overall pest pressure. Thus, there is a need to develop supplementary tactics for the management of these pests that will be compatible with biological control, as well as more reliable protocols for assessing host plant resistance against the increase in infestation intensity

Clinical Presentation and Outcomes of Kawasaki Disease in Children from Latin America: A Multicenter Observational Study from the REKAMLATINA Network

Objetivos: Describir la presentación clínica, el manejo y los resultados de la enfermedad de Kawasaki (EK) en Latinoamérica y evaluar los indicadores pronósticos tempranos de aneurisma de la arteria coronaria (AAC). Diseño del estudio: Se realizó un estudio observacional basado en el registro de la EK en 64 centros pediátricos participantes de 19 países latinoamericanos de forma retrospectiva entre el 1 de enero de 2009 y el 31 de diciembre de 2013, y de forma prospectiva desde el 1 de junio de 2014 hasta el 31 de mayo de 2017. Se recopilaron datos demográficos, clínicos y de laboratorio iniciales. Se utilizó una regresión logística que incorporaba factores clínicos y la puntuación z máxima de la arteria coronaria en la presentación inicial (entre 10 días antes y 5 días después de la inmunoglobulina intravenosa [IGIV]) para desarrollar un modelo pronóstico de AAC durante el seguimiento (>5 días después de la IGIV). Resultados: De 1853 pacientes con EK, el ingreso tardío (>10 días tras el inicio de la fiebre) se produjo en el 16%, el 25% tuvo EK incompleta y el 11% fue resistente a la IGIV. Entre los 671 sujetos con puntuación z de la arteria coronaria notificada durante el seguimiento (mediana: 79 días; IQR: 36, 186), el 21% presentaba AAC, incluido un 4% con aneurismas gigantes. Un modelo pronóstico simple que utilizaba sólo una puntuación z de la arteria coronaria máxima ≥2,5 en la presentación inicial fue óptimo para predecir la AAC durante el seguimiento (área bajo la curva: 0,84; IC del 95%: 0,80, 0,88). Conclusiones: De nuestra población latinoamericana, la puntuación z de la arteria coronaria ≥2,5 en la presentación inicial fue el factor pronóstico más importante que precedió a la AAC durante el seguimiento. Estos resultados resaltan la importancia de la ecocardiografía temprana durante la presentación inicial de la EK. © 2023 Los autoresObjectives: To describe the clinical presentation, management, and outcomes of Kawasaki disease (KD) in Latin America and to evaluate early prognostic indicators of coronary artery aneurysm (CAA). Study design: An observational KD registry-based study was conducted in 64 participating pediatric centers across 19 Latin American countries retrospectively between January 1, 2009, and December 31, 2013, and prospectively from June 1, 2014, to May 31, 2017. Demographic and initial clinical and laboratory data were collected. Logistic regression incorporating clinical factors and maximum coronary artery z-score at initial presentation (between 10 days before and 5 days after intravenous immunoglobulin [IVIG]) was used to develop a prognostic model for CAA during follow-up (>5 days after IVIG). Results: Of 1853 patients with KD, delayed admission (>10 days after fever onset) occurred in 16%, 25% had incomplete KD, and 11% were resistant to IVIG. Among 671 subjects with reported coronary artery z-score during follow-up (median: 79 days; IQR: 36, 186), 21% had CAA, including 4% with giant aneurysms. A simple prognostic model utilizing only a maximum coronary artery z-score ≥2.5 at initial presentation was optimal to predict CAA during follow-up (area under the curve: 0.84; 95% CI: 0.80, 0.88). Conclusion: From our Latin American population, coronary artery z-score ≥2.5 at initial presentation was the most important prognostic factor preceding CAA during follow-up. These results highlight the importance of early echocardiography during the initial presentation of KD. © 2023 The Author(s