Critical Realist Information Systems Research in Action

Part 7: Section 6: The Future of Critical Realism in IS ResearchInternational audienceThere is a growing interest in critical realism (CR) in Information Systems (IS) research. This paper presents and discusses how critical realism can be an alternative philosophical underpinning for IS research. It briefly presents critical realism and how it can be used in IS research. Contemporary examples of how CR have been used in IS research are presented and discussed. The future use of CR in IS research is also discussed

Assignment of 22 loci in the rat by somatic hybrid and linkage analysis.

Twenty structural genes and two unique anonymous DNA fragments have been mapped in the rat (Rattus norvegicus) with a panel of mouse x rat hybrids and linkage analysis. Ten of the 20 autosomes are represented by at least one of these markers. A new syntenic relationship among rat Chromosome (Chr) 16, mouse Chr 14, and human Chr 10q was established. Results of this study further support the extensive conservation of synteny between the rat and mouse and, to a lesser degree, between rat and human.Journal ArticleResearch Support, Non-U.S. Gov'tResearch Support, U.S. Gov't, P.H.S.info:eu-repo/semantics/publishe

Histone Deacetylase Inhibitors Trigger a G2 Checkpoint in Normal Cells That Is Defective in Tumor Cells

Important aspects of cell cycle regulation are the checkpoints, which respond to a variety of cellular stresses to inhibit cell cycle progression and act as protective mechanisms to ensure genomic integrity. An increasing number of tumor suppressors are being demonstrated to have roles in checkpoint mechanisms, implying that checkpoint dysfunction is likely to be a common feature of cancers. Here we report that histone deacetylase inhibitors, in particular azelaic bishydroxamic acid, triggers a G2 phase cell cycle checkpoint response in normal human cells, and this checkpoint is defective in a range of tumor cell lines. Loss of this G2 checkpoint results in the tumor cells undergoing an aberrant mitosis resulting in fractured multinuclei and micronuclei and eventually cell death. This histone deacetylase inhibitor-sensitive checkpoint appears to be distinct from G2/M checkpoints activated by genotoxins and microtubule poisons and may be the human homologue of a yeast G2 checkpoint, which responds to aberrant histone acetylation states. Azelaic bishydroxamic acid may represent a new class of anticancer drugs with selective toxicity based on its ability to target a dysfunctional checkpoint mechanism in tumor cells