ASSESSMENT OF IN VITRO ANTIOXIDANT POTENTIAL AND QUANTIFICATION OF TOTAL PHENOLS AND FLAVONOIDS OF AQUEOUS EXTRACT OF PHYLLANTHUS AMARUS

Objective: The modern research is directed towards finding naturally occurring antioxidants of plant origin. To search for new sources of safe and inexpensive antioxidants, present study was undertaken to quantify total phenols and flavonoids and, evaluation and correlation of antioxidant potential of aqueous extract of Phyllanthus amarus Schum & Thonn, (PAAEt) belongs to Euphorbiace family under in vitro condition.Methods: Determination of total phenolic and flavonoid contents was performed by UV-visible spectrophotometer. The antioxidant potential of P. amarus was tested with different antioxidant test systems; inhibition of lipid peroxidation (LPO), scavenging of 1,1-Diphenyl-2-picrylhydrazyl (DPPH), hydroxyl, superoxide and nitric oxide radicals with reducing power. Results: The plant extract has appreciable amounts of total phenolic compounds (120±2.0 mg GA eq. g-1) and flavonoids (3.02±0.13 mg Q eq. g-1) of the extract. PAAEt exhibited effective and strong antioxidant activity in dose-dependent manners in inhibiting LPO (IC50 623.4 μg/ml) and scavenging of free radicals such as DPPH (IC50 200.2 μg/ml), hydroxyl (IC50 280.6 μg/ml), superoxide (IC50 201.5 μg/ml), nitric oxide (IC50 408.5 μg/ml) with reducing power (IC50 500.0 μg/ml). PAAEt showed effective and strong positive correlation with reducing power (r2= 0.972) and LPO inhibition (r2= 0.964) and free-radicals (P<0.05).Conclusion: Presence of high amount of phenolic and flavonoid compounds and appreciable amounts of IC50 for various free radicals and inhibition of LPO and reducing power with significant strong positive correlation for scavenging of free radicals, LPO inhibition and reducing power indicated that PAAEt has strong antioxidant potential.Â

Effect of Commiphora mukul gum resin on hepatic and renal marker enzymes, lipid peroxidation and antioxidants status in pancreas and heart in fructose fed insulin resistant rats

This work aims to study the antioxidant efficacy of Commiphora mukul (C. mukul) gum resin ethanolic extract in high fructose diet (HFD) insulin resistant rats. The male Wistar albino rats were randomly divided into four groups of eight animals each; two of these groups (Control group [C] and Control treated with C. mukul [C + CM]) were fed with standard pellet diet and the other two groups (Fructose fed rats [F-group] and fructose fed with C. mukul treated group [F + CM]) were fed with high fructose diet (HFD) (66%). C. mukul gum resin ethanolic extract (200 mg/kg body weight/day) was administered orally to group C + CM and group F + CM. At the end of 60-day experimental period biochemical parameters related to antioxidant, oxidative stress marker enzymes and hepatic and renal marker enzymes of tissues were performed. The fructose fed rats showed increased level of enzymatic activities aspartate aminotransminases (AST), alanine aminotransminases (ALT) in liver and kidney and oxidative markers like lipid peroxidation (LPO) and protein oxidation (PO) in pancreas and heart. Antioxidant enzyme activities were significantly decreased in the pancreas and heart compared to control groups. Administration of C. mukul (200 mg/kg bwt) to fructose fed insulin resistant rats for 60 days significantly reversed the above parameters toward normal. In conclusion, our data indicate the preventive role of C. mukul against fructose-induced insulin resistance and oxidative stress; hence this plant could be used as an adjuvant therapy for the prevention and/or management of chronic diseases characterized by hyperinsulinemia, insulin resistance and aggravated antioxidant status