Investigation of effects of three candidate genes on leg action and fat deposition traits in pigs

Data from 188 sows were used in the current study to examine the effects of high mobility group AT-hook1 (HMGA1), transcription factor 7-like-2 (TCF7L2) and insulin-like growth factor binding protein 3 (IGFBP3) genes on leg action and fat deposition traits, and further to explore the possible relationships between these genes on both traits. The candidate genes used in the study are known for their roles in fat deposition and growth. Overall leg action was scored on a scale of 1 (good movement) to 9 (leg weakness). Fatness traits included 10th rib backfat (BF10), adjusted 10th rib backfat to 125 kg (adjBF10) and last rib backfat (last BF), measured by ultrasonic imaging approach. The association analyses between single nucleotide polymorphisms (SNPs) and traits were performed using PROC MIXED procedures of SAS. The results showed that the associations between HMGA1, TCF7L2 and IGFBP3 genotypes with fat deposition traits were mostly suggestive in this limited data set. Leg action was also suggestively associated with IGFBP3 gene effects but was not associated with HMGA1 and TCF7L2 genes. Thus, IGFBP3 AA homozygote individuals tended to have had better movements (5.40), and were fatter when compared to GG homozygotes (5.84). The results from this study suggest a possible association between the IGFBP3 gene effects on both leg action and fatness. Therefore, further studies must be carried out in several populations, and using larger data to demonstrate these results conclusively

Revised Approaches to Estimate Lean of Pork Carcasses of Known Age or Days on Test

Carcass measurements for 185 market hogs representing two sexes and four body types, slaughtered at 91 to 132 kg, were examined as predictors of carcass composition. Dependent variables included weight of fat-standardized lean (FSL), percentage FSL in the standardized side, weight of FSL gained/day on test, and weight of FSL produced/day of age. The greatest degree of predictive accuracy in each equation occurred when longissimus muscle area and fat depth at the three-fourths location at the 10th rib were included as independent variables. Other important variables were hot carcass weight in the three equations predicting weight for FSL, age in the equation for FSL produced/ day of age, and initial weight on test and days on test for the prediction of FSL gained/day on test. Less accuracy was found when other back-fat thickness measurements or subjective scores of muscling or fatness were used as independent variables

Active water in protein-protein communication within the membrane: the case of SRII-HtrII signal relay.

We detect internal water molecules in a membrane-embedded receptor-transducer complex and demonstrate water structure changes during formation of the signaling state. Time-resolved FTIR spectroscopy reveals stimulus-induced repositioning of one or more structurally active water molecules to a significantly more hydrophobic environment in the signaling state of the sensory rhodopsin II (SRII)-transducer (HtrII) complex. These waters, distinct from bound water molecules within the SRII receptor, appear to be in the middle of the transmembrane interface region near the Tyr199(SRII)-Asn74(HtrII) hydrogen bond. We conclude that water potentially plays an important role in the SRII --\u3e HtrII signal transfer mechanism in the membrane\u27s hydrophobic core

Beyond Unprecedented S3 Ep2 ESG: Losing Its Cool

The corporate environmental, social, and governance (ESG) movement has come under fire. After many large corporations began to adopt specific ESG measures in response to shareholder pressure, other shareholder groups are trying to undo them. Inclusive Capital Partners co-founders Lynn Forester de Rothschild ’79 and Jeff Ubben discuss the rise in “anti-woke” shareholder activism, the politicization of ESG, and what lies ahead.https://scholarship.law.columbia.edu/beyond_unprecedented_3/1002/thumbnail.jp

HST Observations of SGR 0526-66: New Constraints on Accretion and Magnetar Models

Soft Gamma-ray Repeaters (SGRs) are among the most enigmatic sources known today. Exhibiting huge X- and Gamma-ray bursts and flares, as well as soft quiescent X-ray emission, their energy source remains a mystery. Just as mysterious are the Anomalous X-ray pulsars (AXPs), which share many of the same characteristics. Thanks to recent Chandra observations, SGR 0526-66, the first SGR, now appears to be a transition object bridging the two classes, and therefore observations of it have implications for both SGRs and AXPs. The two most popular current models for their persistent emission are accretion of a fossil disk or decay of an enormous (~10^15 G) magnetic field in a magnetar. We show how deep optical observations of SGR 0526-66, the only SGR with small enough optical extinction for meaningful observations, show no evidence of an optical counterpart. These observation place strong new constraints on both accretion disk and magnetar models, and suggest that the spectral energy distribution may peak in the hard-UV. Almost all accretion disks are excluded by the optical data, and a magnetar would require a ~10^15-10^16 G field.Comment: 23 pages, 5 figures. Accepted by Ap

Staphylococcus aureus nasal colonization in HIV-seropositive and HIV-seronegative drug users

Nasal colonization plays an important role in the pathogenesis of Staphylococcus aureus infections. To identify characteristics associated with colonization, we studied a cross-section of a well-described cohort of HIV-seropositive and -seronegative active and former drug users considered at risk for staphylococcal infections. Sixty percent of the 217 subjects were Hispanic, 36% were women, 25% actively used injection drugs, 23% actively used inhalational drugs, 23% received antibiotics, and 35% were HIV-seropositive. Forty-one percent of subjects had positive nasal cultures for S. aureus. The antibiotic susceptibility patterns were similar to the local hospital's outpatient isolates and no dominant strain was identified by arbitrarily primed polymerase chain reaction (AB-PCR). Variables significantly and independently associated with colonization included antibiotic use (odds ratio [OR] = 0.37; confidence interval [CI] = 0.18-0.77), active inhalational drug use within the HIV-seropositive population (OR = 2.36; CI = 1.10-5.10) and female gender (OR = 1.97; CI = 1.09-3.57). Characteristics not independently associated included injection drug use, HIV status, and CD4 count. The association with active inhalational drug use, a novel finding, may reflect alterations in the integrity of the nasal mucosa. The lack of association between HIV infection and S. aureus colonization, which is contrary to most previous studies, could be explained by our rigorous control for confounding variables or by a limited statistical power due to the sample sizes

A whole genome scan for quantitative trait loci for leg weakness and its related traits in a large F2 intercross population between White Duroc and Erhualian

To detect QTL for leg weakness and its related traits in pigs, a total of 1,484 F2 pigs were recorded for leg (at 76 and 213 d) and gait scores (at 153 and 223 d) in a White Duroc × Erhualian intercross. The length and weight of the biceps brachii muscle were measured after slaughter at 240 d. A genome scan was performed with 183 microsatellite markers in the population. A total of 42 QTL were detected, including 16 at the 1% genome-wide significant level and 6 at the 5% genome-wide significant level. Thirty-eight of the 42 QTL showed significant additive effects, and 14 had significant dominance effects. At least 2 QTL were detected for each trait except for leg score at 76 d, for which no QTL was identified. Some of the QTL for leg and gait scores confirmed previous findings. Eighteen QTL were detected for weight and length of the biceps brachii muscle. To our knowledge, this was the first report about QTL for weight and length of the biceps brachii muscle in pigs. Two chromosome regions each on SSC4 and SSC7 showed significant and multiple associations with both leg weakness and growth of the biceps brachii muscle, which are worthwhile for further investigation