Influence of the Pentaphylloides fruticosa l. liniment on TBA-active products and antioxidant status in wound injuries associated with experimental diabetes mellitus

The aim of the work was to evaluate the influence of the linimentfrom Pentaphylloides fruticosa L. on lipid peroxidation in rats with experimental diabetes mellitus accompanied by wound process. The liniment was developed with the use of NanoDeBee30 device. Experiments were carried out on white male Wistar rats. Experimental diabetes mellitus was simulated by a single intraperitoneal introduction of alloxan in a dose of 100 mg/kg. The skin injury was simulated by inflicting planar wounds 2.0 x 2.0 cm in size. 5 mg of the 5%-liniment from Pentaphylloides fruticosa was put on the wounds of the first experimental group once a day for 14 days. The second experimental group was treated by the preparation of comparison - balsamic liniment. The wounds of the control group were treated with glycerin-lanolin ointment. On the 7th, 14th and 21st day the concentration of TBA-active products in the blood serum was determined to estimate the intensity of lipid peroxidation processes; the contents of reduced glutathione and catalase activity were determined to evaluate the antioxidant status. The results of the experiments verify the marked curative effect of the linimentfrom Pentaphylloides fruticosa L. on the healing of the wounds on the background of experimental diabetes mellitus in rat

PANCREATODUODENAL RESECTION IN THE TREATMENT OF CHRONIC PANCREATITIS AND PANCREATIC CANCER

A clinical analysis of 51 patient treated, in surgical ward of Republican. Clinical Hospital named, after N.A. Semashko during the period, of 1993—2011. All patients had. pancreatoduodenal resection for chronic pancreatitis (7) and pancreatic cancer (44). In the nearest postoperative period, mortality was 7,8 %. In the remote postoperative period, terms of survivability in 8 patients with cancer were 18—34 months. One patient lived 10 years

Influence of soft finished pharmaproducts based on Pentaphylloides fruticosa l. on the course of wound process

The aim of the work was to estimate a wound-healing effect of soft drug products from the Pentaphylloides fruticosa L. shoots on the model of planar skin-fascial wound. The liniment was developed with the use of the NanoDeBee 30 device and the ointment was developed with the use of pharmaceutical technology. Experiments were carried out on white male Wistar rats. The skin injury was simulated by inflicting planar wounds 2.0 x 2.0 cm in size. The 5%-liniment and 5% ointment from P. fruticosa were put on the wounds of the first and second experimental groups respectively. In the third group the wounds were treated with glycerin-vaseline ointment. Bioptate of the wound surface was taken on the 7th, 14th and 21st day. Histological preparations were studied using standard methods. Cell elements of the first group animals were displayed as fibroblasts and fibrocytes; it was noted high level of collagen organization that testified the effective staging of the wound process in this case. The amount of fibrocytes and fibroblasts and ratio between juvenile and mature forms of collagen-producing cells vary in the first and second groups. Biopsy material obtained from the animals of the third group only on the 21st day corresponded to the picture observed in animals of the 1st group on the 14th day. The findings of the study verify that the use of the product obtained on NanoDeBee device provides the earlier forming of the scar as compared with the ointment developed using pharmaceutical technology

Phthalates in Surface Waters of the Selenga River (Main Tributary of Lake Baikal) and Its Delta: Spatial-Temporal Distribution and Environmental Risk Assessment

The Selenga River provides about half of the water and chemical runoff into Lake Baikal and plays an important role in the sustainability of the ecosystem of this large natural freshwater lake. Phthalate esters (PAEs) are organic compounds that can disrupt reproductive and endocrine systems. This study focused on investigating the distribution of six priority phthalates in the Selenga River and its delta utilizing SPE-GC/MS. The study found that the highest levels of Σ6PAE were observed during the high-water years, 2021 and 2023, and were evenly distributed along the river from the sampling sites upstream of Ulan-Ude to the delta channels. In contrast, the mean annual Σ6PAE content was relatively low in the low water period of 2022. Dibutyl phthalate (DBP) and di-(2-ethylhexyl) phthalate (DEHP) are the two dominant phthalates found in the surface waters of the Selenga River and delta channels. In 2021, the average total concentration of six phthalates (Σ6PAE) ranged from 8.84 to 25.19 µg/L, while in 2022 it ranged from 0.45 to 4.01 µg/L, and in 2023 it ranged from 5.40 to 21.08 µg/L. The maximum level for the sum of phthalates was 61.64 µg/L in 2021, 13.57 µg/L in 2022, and 30.19 µg/L in 2023. The wastewater treatment facilities in Ulan-Ude were identified as a stable local source of phthalates. In some cases, PAE concentrations exceeded maximum allowable concentrations, particularly for DEHP. This could have adverse effects on aquatic organisms