Sex determination from adult human humerus by discriminant function analysis

Background: Sex determination of unidentified skeletal remains from crime scenes or excavation sites is an important component in the development of the biological profile in forensics, anthropology and bioarchaeology. The purpose of this research is to determine whether sexing of unknown adult human humerus bones can be done by applying values of morphometric parameters and formulae generated by present study on adult human humerus bones of known sex and to find out the best parameters for sex determination.Methods: Various metric measurements were recorded using osteo metric board, measuring tape, non-elastic thread, sliding calipers and Vernier calipers on adult human humerus bones.Results: Sex was correctly estimated by using stepwise discriminant function analysis, for the clavicle 100% of males and 95% of females, with a total accuracy of 98.1%. Direct discriminant function analysis, correct estimated sex for the clavicle was 100% in males and 95% in females with a total accuracy of 98.1%.Conclusions: Present study exhibited better classification accuracy for multiple variables than those of single variables, the most discriminating variables in stepwise analysis are the weight, total length, transverse diameter of head, circumference of midshaft, trochlear width, capitulum width. In direct analysis, the single most useful variable was the transverse diameter of head

Determination of sex of adult human clavicle by discriminant function analysis in Marathwada region of Maharashtra

Background: Determination of biological sex is one of the most important determinations to be made from human remains and is an essential first step in the development of the biological profile in forensics, anthropology and bioarchaeology. The aim of this study was to determine whether sexing of unknown adult human clavicles can be done by applying values of morphometric parameters and formulae generated by present study on adult human clavicles of known sex and to find out the best parameters for sex determination.Methods: Various metric measurements were recorded using osteo metric board, measuring tape, non-elastic thread, sliding calipers and vernier calipers on adult human clavicles.Results: Sex was correctly estimated by using stepwise discriminant function analysis, for the clavicle 93.3 % of males and 94.4% of females, with a total accuracy of 93.7 %. Direct discriminant function analysis, correct estimated sex for the clavicle was 93.9 % in males and 93.3% in females with a total accuracy of 93.7 %.Conclusions: Present study exhibited better classification accuracy for multiple variables than those of single variables. In the clavicle, the most discriminating variables in stepwise analysis are the mid clavicular circumference, posterior curved length, medial 2/3 and lateral 1/3 junction circumference and Weight. In direct analysis, the single most useful variable was the mid clavicular circumference

Prediction of Cardiovascular Diseases by Integrating Electrocardiogram (ECG) and Phonocardiogram (PCG) Multi-Modal Features using Hidden Semi Morkov Model

Because the health care field generates a large amount of data, we must employ modern ways to handle this data in order to give effective outcomes and make successful decisions based on data. Heart diseases are the major cause of mortality worldwide, accounting for 1/3th of all fatalities. Cardiovascular disease detection can be accomplished by the detection of disturbance in cardiac signals, one of which is known as phonocardiography. The aim of this project is for using machine learning to categorize cardiac illness based on electrocardiogram (ECG) and phonocardiogram (PCG) readings. The investigation began with signal preprocessing, which included cutting and normalizing the signal, and was accompanied by a continuous wavelet transformation utilizing a mother wavelet analytic morlet. The results of the decomposition are shown using a scalogram, and the outcomes are predicted using the Hidden semi morkov model (HSMM). In the first phase, we submit the dataset file and choose an algorithm to run on the chosen dataset. The accuracy of each selected method is then predicted, along with a graph, and a modal is built for the one with the max frequency by training the dataset to it. In the following step, input for each cardiac parameter is provided, and the sick stage of the heart is predicted based on the modal created. We then take measures based on the patient's condition. When compared to current approaches, the suggested HSMM has 0.952 sensitivity, 0.92 specificity, 0.94 F-score, 0.91 ACC, and 0.96 AUC

Clinical and functional outcomes of pre-contoured plate fixation in displaced intraarticular calcaneum fracture

Background: We evaluated the clinical and functional outcome of intra-articular calcaneal fractures treated with pre-contoured plating. The functional outcome was measured by the American Orthopaedic Foot and Ankle Society (AOFAS) scoring system.Methods: Twenty-five adult patients with displaced intra-articular calcaneus fractures, treated with by open reduction and internal fixation using pre-contoured plates, and those who were available for follow up were evaluated for the outcomes such as operative procedure, anatomical reduction, postoperative hospital stays early mobilization and functional outcome postoperatively. Follow-up was at three, six and twelve months radiological and clinical union were also considered.Results: Twenty-five adult patients presented with calcaneal fractures were operated during the study period. The mean time for union of fracture was 12.5±1.8 weeks. When we assessed the functional outcome in those patients who had radiological and clinical union using AOFAS score, we found that 16 out of 23 (64%) had good results with mean AOFAS score of 83.5±2.0 and 7/25 (28%) had fair results with mean score of 73.3±1.3, and 2/25 (8%) had poor results with mean score of 54.0±8.5. In 2 out of 16 (12.5%) patients, with good AOFAS scores, implants were removed after one and a half year from the day of surgery after confirming complete union of the fracture.Conclusions: We believe that pre-contoured reconstruction plate fixation for displaced intraarticular calcaneus fracture is excellent in terms of the union of the fracture, attaining anatomical reduction. It gives better outcomes concerning the function and range of movements

Image Compression and Watermarking scheme using Scalar Quantization

This paper presents a new compression technique and image watermarking algorithm based on Contourlet Transform (CT). For image compression, an energy based quantization is used. Scalar quantization is explored for image watermarking. Double filter bank structure is used in CT. The Laplacian Pyramid (LP) is used to capture the point discontinuities, and then followed by a Directional Filter Bank (DFB) to link point discontinuities. The coefficients of down sampled low pass version of LP decomposed image are re-ordered in a pre-determined manner and prediction algorithm is used to reduce entropy (bits/pixel). In addition, the coefficients of CT are quantized based on the energy in the particular band. The superiority of proposed algorithm to JPEG is observed in terms of reduced blocking artifacts. The results are also compared with wavelet transform (WT). Superiority of CT to WT is observed when the image contains more contours. The watermark image is embedded in the low pass image of contourlet decomposition. The watermark can be extracted with minimum error. In terms of PSNR, the visual quality of the watermarked image is exceptional. The proposed algorithm is robust to many image attacks and suitable for copyright protection applications.Comment: 11 Pages, IJNGN Journal 201

Radiation and Mass Transfer Effects on Transient Free Convection Flow of a Dissipative Fluid past Semi-Infinite Vertical Plate with Uniform Heat and Mass Flux

Effect of radiation and mass transfer on the transient free convection flow of a dissipative past semi-infinite vertical plate with uniform heat and mass flux is analyzed, by taking into account the effect of viscous dissipation. This type of problems finds application in many technological and engineering fields such as plasma studies, petroleum industries, MHD energy generators, cooling of nuclear reactors, the boundary layer control in aerodynamics, crystal growth and furnace engineering. The Rosseland approximation is used to describe the radiative heat transfer in the limit of the optically thick fluid. The non-linear, coupled equations are solved using an implicit finite difference scheme of Crank-Nicolson type. Transient temperature, concentration and velocity profiles, local and average skinfriction coefficient, Nusselt number and Sherwood number are presented graphically and discussed. It is observed that, when the radiation parameter increases the velocity and temperature decrease accompanied by simultaneous reduction in both momentum and thermal boundary layers

Genetic affinities among the lower castes and tribal groups of India: inference from Y chromosome and mitochondrial DNA

BACKGROUND: India is a country with enormous social and cultural diversity due to its positioning on the crossroads of many historic and pre-historic human migrations. The hierarchical caste system in the Hindu society dominates the social structure of the Indian populations. The origin of the caste system in India is a matter of debate with many linguists and anthropologists suggesting that it began with the arrival of Indo-European speakers from Central Asia about 3500 years ago. Previous genetic studies based on Indian populations failed to achieve a consensus in this regard. We analysed the Y-chromosome and mitochondrial DNA of three tribal populations of southern India, compared the results with available data from the Indian subcontinent and tried to reconstruct the evolutionary history of Indian caste and tribal populations. RESULTS: No significant difference was observed in the mitochondrial DNA between Indian tribal and caste populations, except for the presence of a higher frequency of west Eurasian-specific haplogroups in the higher castes, mostly in the north western part of India. On the other hand, the study of the Indian Y lineages revealed distinct distribution patterns among caste and tribal populations. The paternal lineages of Indian lower castes showed significantly closer affinity to the tribal populations than to the upper castes. The frequencies of deep-rooted Y haplogroups such as M89, M52, and M95 were higher in the lower castes and tribes, compared to the upper castes. CONCLUSION: The present study suggests that the vast majority (>98%) of the Indian maternal gene pool, consisting of Indio-European and Dravidian speakers, is genetically more or less uniform. Invasions after the late Pleistocene settlement might have been mostly male-mediated. However, Y-SNP data provides compelling genetic evidence for a tribal origin of the lower caste populations in the subcontinent. Lower caste groups might have originated with the hierarchical divisions that arose within the tribal groups with the spread of Neolithic agriculturalists, much earlier than the arrival of Aryan speakers. The Indo-Europeans established themselves as upper castes among this already developed caste-like class structure within the tribes

Kloniranje, ekspresija i karakterizacija paraflagelarnog gena Rod 2 bičaša Trypanosoma evansi

Paraflagellar rod is the major structural component of the trypanosomatid flagellum and is identified as a complex lattice of filaments which runs parallel to the axoneme throughout most of the flagellar length. The present study was carried out to investigate the existence of the paraflagellar rod (PFR 2) gene in Trypanosoma evansi infecting Indian cattle. Local isolates of T. evansi collected from naturally infected cow were multiplied in Wistar rats. Complementary DNA (cDNA) was synthesized from the RNA of host cell free T. evansi parasites by reverse transcription. The gel purified PCR product (PFR 2 gene of T. evansi) was cloned into the pTZ57R/T vector system. The nucleotide sequence of the PFR 2 gene of the T. evansi S.V.V.U. isolate (Accession No. KT277497) obtained in the present study revealed 100% homology with T. evansi China isolate and 99% homology with T. evansi Izatnagar and Bikaner isolates. The recombinant protein was sub-cloned into pET 32a and expressed in the BL21 (DE3) pLysS expression system. The PFR 2 gene of T. evansi S.V.V.U. isolate was further characterized by determination of its protein profile with SDS-PAGE and western blotting. Indirect ELISA was optimized for detection of the specific antibody titre against the recombinant protein of the PFR 2 gene of T. evansi. In the kinetoplastid species the PFR 2 gene is highly conserved. Therefore the PFR 2 gene was suggested as a vaccine candidate, as well as a diagnostic antigen.Paraflagelarni štapić glavna je strukturna komponenta tripanosomskog biča i dio je kompleksa filamenaza koji teku paralelno s aksonemom duž biča. Istraživanje je provedeno kako bi se ispitalo postojanje paraflagelarnog gena Rod 2 (PFR2) u bičaša Trypanosoma evansi koji invadira goveda u Indiji. Lokalni izolat T. evansi prikupljen od prirodno invadiranih krava umnožen je u Wistar štakora. Komplementarna DNA (cDNA) sintetizirana je iz RNA obrnutom transkripcijom iz stanica neinvadiranih nositelja T. evansi parazita. Pročišćeni PCR produkt (gen PFR2 bičaša T. evansi) kloniran je u vektorski sustav pTZ57R/T. Nukleotidna sekvencija gena PFR2 bičaša T. evansi, izolat S.V.V.U. (pristupni broj KT277497) dobivena u ovom istraživanju pokazala je 100 %-tnu sličnost s izolatom T. evansi China i 99 %-tnu s izolatom T. evansi Izatnagar i Bikaner. Rekombinantni protein ponovno je kloniran u sustavu pET 32a i prikazan u sustavu BL21 (DE3) pLysS. Gen PFR2 bičaša T. evansi, izolat S.V.V.U. dalje je karakteriziran određivanjem proteinskog profila metodama SDS-PAGE i Western blotting. Indirektni test ELISA optimiziran je za dokaz titra specifičnih protutijela za rekombinantni protein gena PFR2 bičaša T. evansi. U kinetoplastida gen PFR2 izrazito je očuvan. Stoga bi se gen PFR2 mogao upotrijebiti za cjepivo te kao dijagnostički antigen

Role of Alvarado score in the diagnosis of acute appendicitis

Acute appendicitis is a surgical emergency, which is associated with morbidity and mortality occasionally. If failed to diagnose early the situation may become more complicated. In acute appendicitis it is not possible to have definitive diagnosis by histopathology preoperatively, we would like a simple test like Alvarado scoring system which depends on the presence and absence of certain variable, which provides an accurate guide to whether or not the patient has the condition. Hence, accurate diagnosis and timely intervention is needed. Therefore, the present study was conducted to evaluate the Alvarado scoring system in diagnosing acute appendicitis and its co relation with histopathology. This prospective study was conducted in 100 consecutive patients admitted in the department of general surgery of Narayana Medical College, Nellore with the clinical diagnosis of acute appendicitis. The findings based on the Alvarado score indicate that 68% of the subjects had a score of more than 7 and 22% of the subjects had a score between 5 and 7. Only 10% of the subjects had a score between 1 and 4. Out of 83 eligible subjects 75 were found to have acute appendicitis by histopathological examination. Negative appendicectomy was very low representing up to 9.6% whereas the percentage of positive predictive value was high representing up to 90.4%. Although the diagnosis of acute appendicitis remains mainly clinical evaluation, the scoring system is easy, simple and cheap complementary aid for supporting the diagnosis of acute appendicitis

PHARMACOKINETIC DRUG INTERACTION BETWEEN CLOPIDOGREL AND ESOMEPRAZOLE IN ADULT HEALTHY MALE VOLUNTEERS

Objective: Proton pump inhibitors (PPIs) are known to impair cytochrome P2C19 mediated activation of clopidogrel, the antiplatelet agent used for cardiovascular risk prevention. Esomeprazole is an optical isomer of omeprazole with better efficacy and tolerability than conventional PPIs. Esomeprazole is often co-administered with clopidogrel considering the risk of associated gastrointestinal bleeding. This study was designed to determine the effect of esomeprazole on the mean pharmacokinetic profile clopidogrel.Methods: A total of 14 adult healthy male participants who volunteered participation were enrolled, randomized equally into two cross-over sequences, dosed with clopidogrel and clopidogrel + esomeprazole in respective periods. Blood samples were collected through antecubital or forearm vein indwelling catheter. Concentration of clopidogrel parent prodrug in isolated plasma was determined using validated sensitive liquid chromatography – mass spectrometry. Pharmacokinetic modeling was carried out using PKSOLVER add-in for Microsoft Excel.Results: The pharmacokinetic profile of clopidogrel was non-significantly altered by esomeprazole. Statistically significant difference in peak plasma concentration, apparent volume of distribution, and clearance of clopidogrel was observed only during period II in participants co-dosed with esomeprazole (p=0.0483, 0.0011, and 0.0015, respectively). All other primary and secondary pharmacokinetic parameters displayed minor alterations during either period (p>0.05).Conclusion: The non-significant alteration of clopidogrel pharmacokinetics by esomeprazole can be potentiated by underlying predisposing factors such as the presence of CYP2C19 allelic variants and increasing the risk of cardiovascular events. Hence, co-administration of clopidogrel and esomeprazole should be under clinical monitoring and is not recommended in poor responders of antiplatelet therapy with clopidogrel