347 research outputs found
Microscopical methods for the localization of Na + , K + -ATPase
Na + , K + -ATPase plays a central role in the ionic and osmotic homeostasis of cells and in the movements of electrolytes and water across epithelial boundaries. Microscopic localization of the enzyme is, therefore, of crucial importance in establishing the subcellular routes of electrolyte flow across structurally complex and functionally polarized epithelia. Recently developed approaches to the localization of Na + , K + -ATPase are reviewed. These methods rely on different properties of the enzyme and encompass cytochemical localization of the K + -dependent nitrophenylphosphatase component of the enzyme, autoradiographic localization of tritiated ouabain binding sites, and immunocytochemical localization of the holoenzyme and of its catalytic subunit. The rationales for each of these techniques are outlined as are the critieria that have been established to validate each method. The observed localization of Na + , K + -ATPase in various tissues is discussed, particularly as it relates to putative and hypothetical mechanisms that are currently thought to mediate reabsorptive and secretory electrolyte transport.Peer Reviewedhttp://deepblue.lib.umich.edu/bitstream/2027.42/42850/1/10735_2005_Article_BF01005056.pd
Elektronenmikroskopische Darstellung von ATPase-Aktivit�t an den Cristae mitochondriales und anderen Membranstrukturen im Herzmuskel
Zur elektronenmikroskopischen Darstellung der ATP-spaltenden Fermentaktivität in Knochenmarkszellen
Application and Evaluation of the Lead-Adenosine Triphosphatase Method in Skeletal Tissue
TELETHONIN AS AN EARLY IMMUNOHISTOCHEMICAL MARKER IN LIGATION-INDUCED ISCHEMIC MYOCARDIAL INJURY
Variations in the distribution pattern of acid and alkaline phosphatase activity in the kidney of some Selachians
Histochemische untersuchungen �ber die lokalisation und aktivit�t der 5-nucleotidase, adenosintriphosphatase, sauren phosphatase, sauren desoxyribonuclease, alkalischen phosphatase und der unspezifischen esterasen in der leber bei experimenteller manganvergiftung
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